EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM reveals the complex architecture of dynactin's shoulder region and pointed end.
ジャーナル・号・ページ	EMBO J, Vol. 40, Issue 8, Page e106164, Year 2021
掲載日	2021年4月15日
著者	Clinton K Lau / Francis J O'Reilly / Balaji Santhanam / Samuel E Lacey / Juri Rappsilber / Andrew P Carter /
PubMed 要旨	Dynactin is a 1.1 MDa complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In order to do this, it forms a tripartite complex with dynein and a coiled- ...Dynactin is a 1.1 MDa complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In order to do this, it forms a tripartite complex with dynein and a coiled-coil adaptor. Dynactin consists of an actin-related filament whose length is defined by its flexible shoulder domain. Despite previous cryo-EM structures, the molecular architecture of the shoulder and pointed end of the filament is still poorly understood due to the lack of high-resolution information in these regions. Here we combine multiple cryo-EM datasets and define precise masking strategies for particle signal subtraction and 3D classification. This overcomes domain flexibility and results in high-resolution maps into which we can build the shoulder and pointed end. The unique architecture of the shoulder securely houses the p150 subunit and positions the four identical p50 subunits in different conformations to bind dynactin's filament. The pointed end map allows us to build the first structure of p62 and reveals the molecular basis for cargo adaptor binding to different sites at the pointed end.
リンク	EMBO J / PubMed:33734450 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 8.2 Å
構造データ	11313 6znl EMDB-11313: Cryo-EM structure of the dynactin complex at 3.8 Angstrom resolution PDB-6znl: Cryo-EM structure of the dynactin complex 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-11314: The shoulder domain of dynactin with underlying Arp1 filament subnits 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-11315: The pointed end complex of dynactin, with accompanying Arp1/actin filament subunits 手法: EM (単粒子) / 解像度: 4.05 Å EMDB-11316: Map of the shoulder domain from dynactin to resolve the upper paddle and hook regions 手法: EM (単粒子) / 解像度: 4.63 Å 11317 6znm EMDB-11317, PDB-6znm: The pointed end complex of dynactin bound to BICDR1 手法: EM (単粒子) / 解像度: 4.1 Å 11318 6znn EMDB-11318, PDB-6znn: The pointed end complex of dynactin bound to Hook3 手法: EM (単粒子) / 解像度: 4.5 Å 11319 6zno EMDB-11319, PDB-6zno: The pointed end complex of dynactin with the p150 projection docked 手法: EM (単粒子) / 解像度: 6.8 Å PDB-6zo4: The pointed end complex of dynactin bound to BICD2 手法: ELECTRON MICROSCOPY / 解像度: 8.2 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-ZN: Unknown entry
由来	sus scrofa (ブタ) mus musculus (ハツカネズミ) homo sapiens (ヒト)
キーワード	STRUCTURAL PROTEIN (タンパク質) / Dynactin (ダイナクチン) / Complex / Scaffold (足場) / Cytoskeleton (細胞骨格)