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-Structure paper
タイトル | Structures of the human mitochondrial ribosome bound to EF-G1 reveal distinct features of mitochondrial translation elongation. |
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ジャーナル・号・ページ | Nat Commun, Vol. 11, Issue 1, Page 3830, Year 2020 |
掲載日 | 2020年7月31日 |
著者 | Ravi Kiran Koripella / Manjuli R Sharma / Kalpana Bhargava / Partha P Datta / Prem S Kaushal / Pooja Keshavan / Linda L Spremulli / Nilesh K Banavali / Rajendra K Agrawal / |
PubMed 要旨 | The mammalian mitochondrial ribosome (mitoribosome) and its associated translational factors have evolved to accommodate greater participation of proteins in mitochondrial translation. Here we ...The mammalian mitochondrial ribosome (mitoribosome) and its associated translational factors have evolved to accommodate greater participation of proteins in mitochondrial translation. Here we present the 2.68-3.96 Å cryo-EM structures of the human 55S mitoribosome in complex with the human mitochondrial elongation factor G1 (EF-G1) in three distinct conformational states, including an intermediate state and a post-translocational state. These structures reveal the role of several mitochondria-specific (mito-specific) mitoribosomal proteins (MRPs) and a mito-specific segment of EF-G1 in mitochondrial tRNA (tRNA) translocation. In particular, the mito-specific C-terminal extension in EF-G1 is directly involved in translocation of the acceptor arm of the A-site tRNA. In addition to the ratchet-like and independent head-swiveling motions exhibited by the small mitoribosomal subunit, we discover significant conformational changes in MRP mL45 at the nascent polypeptide-exit site within the large mitoribosomal subunit that could be critical for tethering of the elongating mitoribosome onto the inner-mitochondrial membrane. |
リンク | Nat Commun / PubMed:32737313 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.96 - 6.8 Å |
構造データ | EMDB-21233, PDB-6vlz: EMDB-21242, PDB-6vmi: EMDB-22209: EMDB-22212: |
化合物 | ChemComp-MG: ChemComp-ZN: ChemComp-GDP: ChemComp-GCP: |
由来 |
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キーワード | RIBOSOME (リボソーム) / mitochondrial elongation factor-G1 / 55S ribosome |