EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of a mitochondrial ATP synthase with bound native cardiolipin.
ジャーナル・号・ページ	Elife, Vol. 8, Year 2019
掲載日	2019年11月18日
著者	Alexander Mühleip / Sarah E McComas / Alexey Amunts /
PubMed 要旨	The mitochondrial ATP synthase fuels eukaryotic cells with chemical energy. Here we report the cryo-EM structure of a divergent ATP synthase dimer from mitochondria of , a member of the phylum ...The mitochondrial ATP synthase fuels eukaryotic cells with chemical energy. Here we report the cryo-EM structure of a divergent ATP synthase dimer from mitochondria of , a member of the phylum Euglenozoa that also includes human parasites. It features 29 different subunits, 8 of which are newly identified. The membrane region was determined to 2.8 Å resolution, enabling the identification of 37 associated lipids, including 25 cardiolipins, which provides insight into protein-lipid interactions and their functional roles. The rotor-stator interface comprises four membrane-embedded horizontal helices, including a distinct subunit . The dimer interface is formed entirely by phylum-specific components, and a peripherally associated subcomplex contributes to the membrane curvature. The central and peripheral stalks directly interact with each other. Last, the ATPase inhibitory factor 1 (IF) binds in a mode that is different from human, but conserved in Trypanosomatids.
リンク	Elife / PubMed:31738165 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 4.32 Å
構造データ	10467 6tdu EMDB-10467, PDB-6tdu: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, full dimer, rotational states 1 手法: EM (単粒子) / 解像度: 4.32 Å 10468 6tdv EMDB-10468, PDB-6tdv: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, membrane region 手法: EM (単粒子) / 解像度: 2.8 Å 10469 6tdw EMDB-10469, PDB-6tdw: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, peripheral stalk, rotational state 1 手法: EM (単粒子) / 解像度: 3.8 Å 10470 6tdx EMDB-10470, PDB-6tdx: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, rotor, rotational state 1 手法: EM (単粒子) / 解像度: 3.3 Å 10471 6tdy EMDB-10471: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/cring in rotational state 1 PDB-6tdy: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring in rotational state 1 手法: EM (単粒子) / 解像度: 3.04 Å 10472 6tdz EMDB-10472: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/cring rotational state 2 PDB-6tdz: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 2 手法: EM (単粒子) / 解像度: 3.14 Å 10473 6te0 EMDB-10473, PDB-6te0: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3 手法: EM (単粒子) / 解像度: 3.92 Å EMDB-10474: Cryo-EM structure of the Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3 with bound IF1 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-10475: Cryo-EM structure of the Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3 without IF1 手法: EM (単粒子) / 解像度: 4.2 Å
化合物	ChemComp-CDL: CARDIOLIPIN / リン脂質YM / Cardiolipin ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-LPP: 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE / ジパルミトイルホスファチジン酸 / リン脂質YM ChemComp-TRT: FRAGMENT OF TRITON X-100 / 可溶化剤YM / トリトンX-100 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸
由来	euglena gracilis (ミドリムシ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / mitochondria (ミトコンドリア) / ATP synthase (ATP合成酵素)