EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural Analysis of the Bacterial Proteasome Activator Bpa in Complex with the 20S Proteasome.
ジャーナル・号・ページ	Structure, Vol. 24, Issue 12, Page 2138-2151, Year 2016
掲載日	2016年12月6日
著者	Marcel Bolten / Cyrille L Delley / Marc Leibundgut / Daniel Boehringer / Nenad Ban / Eilika Weber-Ban /
PubMed 要旨	Mycobacterium tuberculosis harbors proteasomes that recruit substrates for degradation through an ubiquitin-like modification pathway. Recently, a non-ATPase activator termed Bpa (bacterial ...Mycobacterium tuberculosis harbors proteasomes that recruit substrates for degradation through an ubiquitin-like modification pathway. Recently, a non-ATPase activator termed Bpa (bacterial proteasome activator) was shown to support an alternate proteasomal degradation pathway. Here, we present the cryo-electron microscopy (cryo-EM) structure of Bpa in complex with the 20S core particle (CP). For docking into the cryo-EM density, we solved the X-ray structure of Bpa, showing that it forms tight four-helix bundles arranged into a 12-membered ring with a 40 Å wide central pore and the C-terminal helix of each protomer protruding from the ring. The Bpa model was fitted into the cryo-EM map of the Bpa-CP complex, revealing its architecture and striking symmetry mismatch. The Bpa-CP interface was resolved to 3.5 Å, showing the interactions between the C-terminal GQYL motif of Bpa and the proteasome α-rings. This docking mode is related to the one observed for eukaryotic activators with features specific to the bacterial complex.
リンク	Structure / PubMed:27839949
手法	EM (単粒子) / X線回折
解像度	2.6 - 4.6 Å
構造データ	EMDB-4127: Cryo-EM reconstruction of the Bacterial proteasome interactor (Bpa) bound to the 20S proteasome of M. tuberculosis. 手法: EM (単粒子) / 解像度: 4.6 Å 4128 5lzp EMDB-4128, PDB-5lzp: Binding of the C-terminal GQYL motif of the bacterial proteasome activator Bpa to the 20S proteasome 手法: EM (単粒子) / 解像度: 3.5 Å PDB-5lfj: Crystal Structure of the Bacterial Proteasome Activator Bpa of Mycobacterium tuberculosis 手法: X-RAY DIFFRACTION / 解像度: 2.6 Å PDB-5lfp: Crystal Structure of the Bacterial Proteasome Activator Bpa of Mycobacterium tuberculosis (space group P6322, SeMet) 手法: X-RAY DIFFRACTION / 解像度: 3.303 Å PDB-5lfq: Crystal Structure of the Bacterial Proteasome Activator Bpa of Mycobacterium tuberculosis (space group P3) 手法: X-RAY DIFFRACTION / 解像度: 3.503 Å
化合物	ChemComp-HOH: WATER / 水
由来	mycobacterium tuberculosis h37rv (結核菌) mycobacterium tuberculosis (strain atcc 25618 / h37rv) (結核菌)
キーワード	CHAPERONE (シャペロン) / Dodecamer / four-helix bundle (ヘリックスバンドル) / SIGNALING PROTEIN / HYDROLASE (加水分解酵素) / Proteasome (プロテアソーム) / Proteasome activator / protein degradation (タンパク質分解) / complex