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-Structure paper
タイトル | Molecular rearrangements involved in the capsid shell maturation of bacteriophage T7. |
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ジャーナル・号・ページ | J Biol Chem, Vol. 286, Issue 1, Page 234-242, Year 2011 |
掲載日 | 2011年1月7日 |
著者 | Alina Ionel / Javier A Velázquez-Muriel / Daniel Luque / Ana Cuervo / José R Castón / José M Valpuesta / Jaime Martín-Benito / José L Carrascosa / |
PubMed 要旨 | Maturation of dsDNA bacteriophages involves assembling the virus prohead from a limited set of structural components followed by rearrangements required for the stability that is necessary for ...Maturation of dsDNA bacteriophages involves assembling the virus prohead from a limited set of structural components followed by rearrangements required for the stability that is necessary for infecting a host under challenging environmental conditions. Here, we determine the mature capsid structure of T7 at 1 nm resolution by cryo-electron microscopy and compare it with the prohead to reveal the molecular basis of T7 shell maturation. The mature capsid presents an expanded and thinner shell, with a drastic rearrangement of the major protein monomers that increases in their interacting surfaces, in turn resulting in a new bonding lattice. The rearrangements include tilting, in-plane rotation, and radial expansion of the subunits, as well as a relative bending of the A- and P-domains of each subunit. The unique features of this shell transformation, which does not employ the accessory proteins, inserted domains, or molecular interactions observed in other phages, suggest a simple capsid assembling strategy that may have appeared early in the evolution of these viruses. |
リンク | J Biol Chem / PubMed:20962334 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 10.8 - 10.9 Å |
構造データ | PDB-3izg: |
由来 |
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キーワード | VIRUS (ウイルス) / CAPSID MATURATION / MORPHOGENETIC INTERMEDIATE / bacteriophage (ファージ) / cryoelectron microscopy (低温電子顕微鏡法) / icosahedral (二十面体) |