EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for Y2 receptor-mediated neuropeptide Y and peptide YY signaling.
ジャーナル・号・ページ	Structure, Vol. 31, Issue 1, Page 44-57.e6, Year 2023
掲載日	2023年1月5日
著者	Hyunook Kang / Chaehee Park / Yeol Kyo Choi / Jungnam Bae / Sohee Kwon / Jinuk Kim / Chulwon Choi / Chaok Seok / Wonpil Im / Hee-Jung Choi /
PubMed 要旨	Neuropeptide Y (NPY) and its receptors are expressed in various human tissues including the brain where they regulate appetite and emotion. Upon NPY stimulation, the neuropeptide Y1 and Y2 receptors ...Neuropeptide Y (NPY) and its receptors are expressed in various human tissues including the brain where they regulate appetite and emotion. Upon NPY stimulation, the neuropeptide Y1 and Y2 receptors (YR and YR, respectively) activate G signaling, but their physiological responses to food intake are different. In addition, deletion of the two N-terminal amino acids of peptide YY (PYY(3-36)), the endogenous form found in circulation, can stimulate YR but not YR, suggesting that YR and YR may have distinct ligand-binding modes. Here, we report the cryo-electron microscopy structures of the PYY(3-36)‒YR‒G and NPY‒YR‒G complexes. Using cell-based assays, molecular dynamics simulations, and structural analysis, we revealed the molecular basis of the exclusive binding of PYY(3-36) to YR. Furthermore, we demonstrated that YR favors G protein signaling over β-arrestin signaling upon activation, whereas YR does not show a preference between these two pathways.
リンク	Structure / PubMed:36525977
手法	EM (単粒子)
解像度	2.89 - 3.39 Å
構造データ	33984 7yon EMDB-33984, PDB-7yon: Complex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and Gi 手法: EM (単粒子) / 解像度: 2.95 Å 33985 7yoo EMDB-33985, PDB-7yoo: Complex structure of Neuropeptide Y Y2 receptor in complex with NPY and Gi 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-35494: Complex structure of Neuropeptide Y Y2 receptor in complex with NPY and Gi (Consensus map) 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-35495: Complex structure of Neuropeptide Y Y2 receptor in complex with NPY and Gi (Focused map on NPY-Y2R) 手法: EM (単粒子) / 解像度: 3.39 Å EMDB-35496: Complex structure of Neuropeptide Y Y2 receptor in complex with NPY and Gi (Focused map on Gi-scFv16) 手法: EM (単粒子) / 解像度: 3.04 Å EMDB-35497: Complex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and Gi (Consensus map) 手法: EM (単粒子) / 解像度: 2.95 Å EMDB-35498: Complex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and Gi (Focused map on PYY(3-36)-Y2R) 手法: EM (単粒子) / 解像度: 3.15 Å EMDB-35499: Complex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and Gi (Focused map on Gi-scFv16) 手法: EM (単粒子) / 解像度: 2.89 Å
化合物	ChemComp-HOH: WATER / 水
由来	homo sapiens (ヒト) mus musculus (ハツカネズミ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / NPY / GPCR (Gタンパク質共役受容体) / G-protein (Gタンパク質) / Complex / G-protein coupled receptor (Gタンパク質共役受容体) / neuropeptide y (神経ペプチドY)