EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Coordinated conformational changes in the V complex during V-ATPase reversible dissociation.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 29, Issue 5, Page 430-439, Year 2022
掲載日	2022年4月25日
著者	Thamiya Vasanthakumar / Kristine A Keon / Stephanie A Bueler / Michael C Jaskolka / John L Rubinstein /
PubMed 要旨	Vacuolar-type ATPases (V-ATPases) are rotary enzymes that acidify intracellular compartments in eukaryotic cells. These multi-subunit complexes consist of a cytoplasmic V region that hydrolyzes ATP ...Vacuolar-type ATPases (V-ATPases) are rotary enzymes that acidify intracellular compartments in eukaryotic cells. These multi-subunit complexes consist of a cytoplasmic V region that hydrolyzes ATP and a membrane-embedded V region that transports protons. V-ATPase activity is regulated by reversible dissociation of the two regions, with the isolated V and V complexes becoming autoinhibited on disassembly and subunit C subsequently detaching from V. In yeast, assembly of the V and V regions is mediated by the regulator of the ATPase of vacuoles and endosomes (RAVE) complex through an unknown mechanism. We used cryogenic-electron microscopy of yeast V-ATPase to determine structures of the intact enzyme, the dissociated but complete V complex and the V complex lacking subunit C. On separation, V undergoes a dramatic conformational rearrangement, with its rotational state becoming incompatible for reassembly with V. Loss of subunit C allows V to match the rotational state of V, suggesting how RAVE could reassemble V and V by recruiting subunit C.
リンク	Nat Struct Mol Biol / PubMed:35469063
手法	EM (単粒子)
解像度	3.3 - 3.8 Å
構造データ	25996 7tmm EMDB-25996, PDB-7tmm: Complete V1 Complex from Saccharomyces cerevisiae 手法: EM (単粒子) / 解像度: 3.5 Å 25997 7tmo EMDB-25997, PDB-7tmo: V1 complex lacking subunit C from Saccharomyces cerevisiae, State 1 手法: EM (単粒子) / 解像度: 3.3 Å 25998 7tmp EMDB-25998, PDB-7tmp: V1 complex lacking subunit C from Saccharomyces cerevisiae, State 2 手法: EM (単粒子) / 解像度: 3.3 Å 25999 7tmq EMDB-25999, PDB-7tmq: V1 complex lacking subunit C from Saccharomyces cerevisiae, State 3 手法: EM (単粒子) / 解像度: 3.3 Å 26000 7tmr EMDB-26000, PDB-7tmr: V-ATPase from Saccharomyces cerevisiae, State 1 手法: EM (単粒子) / 解像度: 3.5 Å 26001 7tms EMDB-26001, PDB-7tms: V-ATPase from Saccharomyces cerevisiae, State 2 手法: EM (単粒子) / 解像度: 3.8 Å 26002 7tmt EMDB-26002, PDB-7tmt: V-ATPase from Saccharomyces cerevisiae, State 3 手法: EM (単粒子) / 解像度: 3.8 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae (パン酵母)
キーワード	HYDROLASE (加水分解酵素) / V-ATPase / MEMBRANE PROTEIN (膜タンパク質)