EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryomicroscopy reveals the structural basis for a flexible hinge motion in the immunoglobulin M pentamer.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 6314, Year 2022
掲載日	2022年10月23日
著者	Qu Chen / Rajesh Menon / Lesley J Calder / Pavel Tolar / Peter B Rosenthal /
PubMed 要旨	Immunoglobulin M (IgM) is the most ancient of the five isotypes of immunoglobulin (Ig) molecules and serves as the first line of defence against pathogens. Here, we use cryo-EM to image the structure ...Immunoglobulin M (IgM) is the most ancient of the five isotypes of immunoglobulin (Ig) molecules and serves as the first line of defence against pathogens. Here, we use cryo-EM to image the structure of the human full-length IgM pentamer, revealing antigen binding domains flexibly attached to the asymmetric and rigid core formed by the Cμ4 and Cμ3 constant regions and the J-chain. A hinge is located at the Cμ3/Cμ2 domain interface, allowing Fabs and Cμ2 to pivot as a unit both in-plane and out-of-plane. This motion is different from that observed in IgG and IgA, where the two Fab arms are able to swing independently. A biased orientation of one pair of Fab arms results from asymmetry in the constant domain (Cμ3) at the IgM subunit interacting most extensively with the J-chain. This may influence the multi-valent binding to surface-associated antigens and complement pathway activation. By comparison, the structure of the Fc fragment in the IgM monomer is similar to that of the pentamer, but is more dynamic in the Cμ4 domain.
リンク	Nat Commun / PubMed:36274064 / PubMed Central
手法	EM (単粒子)
解像度	3.6 - 8.5 Å
構造データ	EMDB-13921: Cryo-EM structure of full-length human immunoglobulin M 手法: EM (単粒子) / 解像度: 4.4 Å 13922 7qdo EMDB-13922, PDB-7qdo: Cryo-EM structure of human monomeric IgM-Fc 手法: EM (単粒子) / 解像度: 3.6 Å 15375 8ady EMDB-15375, PDB-8ady: Cryo-EM structure of full-length human immunoglobulin M - F(ab')2 conformation 1 手法: EM (単粒子) / 解像度: 5.2 Å 15376 8adz EMDB-15376, PDB-8adz: Cryo-EM structure of full-length human immunoglobulin M - F(ab')2 conformation 2 手法: EM (単粒子) / 解像度: 6.7 Å 15377 8ae0 EMDB-15377, PDB-8ae0: Cryo-EM structure of full-length human immunoglobulin M - F(ab')2 conformation 3 手法: EM (単粒子) / 解像度: 7.1 Å 15379 8ae2 EMDB-15379, PDB-8ae2: Cryo-EM structure of full-length human immunoglobulin M - F(ab')2 conformation 5 手法: EM (単粒子) / 解像度: 8.5 Å 15380 8ae3 EMDB-15380, PDB-8ae3: Cryo-EM structure of full-length human immunoglobulin M - F(ab')2 conformation 4 手法: EM (単粒子) / 解像度: 6.8 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	homo sapiens (ヒト) human (ヒト)
キーワード	IMMUNE SYSTEM (免疫系) / human monomeric IgM-Fc / full-length human immunoglobulin M