EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	PAXX binding to the NHEJ machinery explains functional redundancy with XLF.
ジャーナル・号・ページ	Sci Adv, Vol. 9, Issue 22, Page eadg2834, Year 2023
掲載日	2023年6月2日
著者	Murielle Seif-El-Dahan / Antonia Kefala-Stavridi / Philippe Frit / Steven W Hardwick / Dima Y Chirgadze / Taiana Maia De Oliviera / Jessica Andreani / Sébastien Britton / Nadia Barboule / Madeleine Bossaert / Arun Prasad Pandurangan / Katheryn Meek / Tom L Blundell / Virginie Ropars / Patrick Calsou / Jean-Baptiste Charbonnier / Amanda K Chaplin /
PubMed 要旨	Nonhomologous end joining is a critical mechanism that repairs DNA double-strand breaks in human cells. In this work, we address the structural and functional role of the accessory protein PAXX ...Nonhomologous end joining is a critical mechanism that repairs DNA double-strand breaks in human cells. In this work, we address the structural and functional role of the accessory protein PAXX [paralog of x-ray repair cross-complementing protein 4 (XRCC4) and XRCC4-like factor (XLF)] in this mechanism. Here, we report high-resolution cryo-electron microscopy (cryo-EM) and x-ray crystallography structures of the PAXX C-terminal Ku-binding motif bound to Ku70/80 and cryo-EM structures of PAXX bound to two alternate DNA-dependent protein kinase (DNA-PK) end-bridging dimers, mediated by either Ku80 or XLF. We identify residues critical for the Ku70/PAXX interaction in vitro and in cells. We demonstrate that PAXX and XLF can bind simultaneously to the Ku heterodimer and act as structural bridges in alternate forms of DNA-PK dimers. Last, we show that engagement of both proteins provides a complementary advantage for DNA end synapsis and end joining in cells.
リンク	Sci Adv / PubMed:37256950 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.68 - 5.33 Å
構造データ	14995 7zwa EMDB-14995, PDB-7zwa: CryoEM structure of Ku heterodimer bound to DNA and PAXX 手法: EM (単粒子) / 解像度: 2.8 Å 15022 7zyg EMDB-15022, PDB-7zyg: CryoEM structure of Ku heterodimer bound to DNA, PAXX and XLF 手法: EM (単粒子) / 解像度: 2.68 Å 16044 8bh3 EMDB-16044, PDB-8bh3: DNA-PK Ku80 mediated dimer bound to PAXX 手法: EM (単粒子) / 解像度: 4.55 Å 16070 8bhv EMDB-16070, PDB-8bhv: DNA-PK XLF mediated dimer bound to PAXX 手法: EM (単粒子) / 解像度: 4.51 Å 16074 8bhy EMDB-16074, PDB-8bhy: DNA-PK Ku80 mediated dimer bound to PAXX and XLF 手法: EM (単粒子) / 解像度: 5.33 Å PDB-8asc: Ku70/80 binds to the Ku-binding motif of PAXX 手法: X-RAY DIFFRACTION / 解像度: 2.95 Å
化合物	ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩 ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-HOH: WATER / 水
由来	homo sapiens (ヒト) human (ヒト) dna molecule (デオキシリボ核酸)
キーワード	DNA BINDING PROTEIN (DNA結合タンパク質) / NHEJ (非相同末端結合) / Ku70 (Ku70) / Ku80 (Ku80) / DNA damage (DNA修復) / PAXX / XLF / DNA repair (DNA修復) / DNA-PK / DNA-PKcs (DNA依存性プロテインキナーゼ触媒サブユニット)