EMDB/PDB/SASBDBから引用されている文献のデータベース

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構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
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IF	>30 >20 >10 >5 all

タイトル	Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 3226, Year 2021
掲載日	2021年5月28日
著者	Martin Obr / Clifton L Ricana / Nadia Nikulin / Jon-Philip R Feathers / Marco Klanschnig / Andreas Thader / Marc C Johnson / Volker M Vogt / Florian K M Schur / Robert A Dick /
PubMed 要旨	Inositol hexakisphosphate (IP6) is an assembly cofactor for HIV-1. We report here that IP6 is also used for assembly of Rous sarcoma virus (RSV), a retrovirus from a different genus. IP6 is ~100-fold ...Inositol hexakisphosphate (IP6) is an assembly cofactor for HIV-1. We report here that IP6 is also used for assembly of Rous sarcoma virus (RSV), a retrovirus from a different genus. IP6 is ~100-fold more potent at promoting RSV mature capsid protein (CA) assembly than observed for HIV-1 and removal of IP6 in cells reduces infectivity by 100-fold. Here, visualized by cryo-electron tomography and subtomogram averaging, mature capsid-like particles show an IP6-like density in the CA hexamer, coordinated by rings of six lysines and six arginines. Phosphate and IP6 have opposing effects on CA in vitro assembly, inducing formation of T = 1 icosahedrons and tubes, respectively, implying that phosphate promotes pentamer and IP6 hexamer formation. Subtomogram averaging and classification optimized for analysis of pleomorphic retrovirus particles reveal that the heterogeneity of mature RSV CA polyhedrons results from an unexpected, intrinsic CA hexamer flexibility. In contrast, the CA pentamer forms rigid units organizing the local architecture. These different features of hexamers and pentamers determine the structural mechanism to form CA polyhedrons of variable shape in mature RSV particles.
リンク	Nat Commun / PubMed:34050170 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均) / EM (トモグラフィー)
解像度	3.1 - 9.1 Å
構造データ	12485 7no0 EMDB-12485, PDB-7no0: Structure of the mature RSV CA lattice: T=1 CA icosahedron 手法: EM (単粒子) / 解像度: 3.1 Å 12486 7no1 EMDB-12486, PDB-7no1: Structure of the mature RSV CA lattice: T=3 CA icosahedron 手法: EM (単粒子) / 解像度: 7.6 Å 12487 7no2 EMDB-12487, PDB-7no2: Structure of the mature RSV CA lattice: hexamer derived from tubes (C2-symmetric) 手法: EM (サブトモグラム平均) / 解像度: 4.3 Å 12488 7no3 EMDB-12488, PDB-7no3: Structure of the mature RSV CA lattice: pentamer derived from polyhedral VLPs 手法: EM (サブトモグラム平均) / 解像度: 5.8 Å 12489 7no4 EMDB-12489, PDB-7no4: Structure of the mature RSV CA lattice: hexamer with 3 adjacent pentamers (C3 symmetric) 手法: EM (サブトモグラム平均) / 解像度: 7.5 Å 12490 7no5 EMDB-12490, PDB-7no5: Structure of the mature RSV CA lattice: hexamer with 2 adjacent pentamers (C2 symmetric) 手法: EM (サブトモグラム平均) / 解像度: 7.4 Å 12491 7no6 EMDB-12491, PDB-7no6: Structure of the mature RSV CA lattice: Group I, pentamer-hexamer interface, class 1 手法: EM (サブトモグラム平均) / 解像度: 6.0 Å 12492 7no7 EMDB-12492, PDB-7no7: Structure of the mature RSV CA lattice: Group I, pentamer-hexamer interface, class 1"2 手法: EM (サブトモグラム平均) / 解像度: 7.5 Å 12493 7no8 EMDB-12493, PDB-7no8: Structure of the mature RSV CA lattice: Group I, pentamer-hexamer interface, class 1"6 手法: EM (サブトモグラム平均) / 解像度: 7.9 Å 12494 7no9 EMDB-12494, PDB-7no9: Structure of the mature RSV CA lattice: Group I, pentamer-pentamer interface, class 1'1 手法: EM (サブトモグラム平均) / 解像度: 7.6 Å 12495 7noa EMDB-12495, PDB-7noa: Structure of the mature RSV CA lattice: Group II, hexamer-hexamer interface, class 6 手法: EM (サブトモグラム平均) / 解像度: 6.4 Å 12496 7nob EMDB-12496, PDB-7nob: Structure of the mature RSV CA lattice: Group II, hexamer-hexamer interface, class 2'6 手法: EM (サブトモグラム平均) / 解像度: 6.7 Å 12497 7noc EMDB-12497, PDB-7noc: Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 3'3 手法: EM (サブトモグラム平均) / 解像度: 7.8 Å 12498 7nod EMDB-12498, PDB-7nod: Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 3'4 手法: EM (サブトモグラム平均) / 解像度: 7.8 Å 12499 7noe EMDB-12499, PDB-7noe: Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 3'5 手法: EM (サブトモグラム平均) / 解像度: 7.5 Å 12500 7nof EMDB-12500, PDB-7nof: Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 4'4 手法: EM (サブトモグラム平均) / 解像度: 7.6 Å 12501 7nog EMDB-12501, PDB-7nog: Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 4'5 手法: EM (サブトモグラム平均) / 解像度: 7.8 Å 12502 7noh EMDB-12502, PDB-7noh: Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 5'5 手法: EM (サブトモグラム平均) / 解像度: 7.1 Å 12503 7noi EMDB-12503, PDB-7noi: Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 3'Alpha 手法: EM (サブトモグラム平均) / 解像度: 7.2 Å 12504 7noj EMDB-12504, PDB-7noj: Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 3'Beta 手法: EM (サブトモグラム平均) / 解像度: 6.7 Å 12505 7nok EMDB-12505, PDB-7nok: Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 3'Gamma 手法: EM (サブトモグラム平均) / 解像度: 9.1 Å 12506 7nol EMDB-12506, PDB-7nol: Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 4'Alpha 手法: EM (サブトモグラム平均) / 解像度: 8.2 Å 12507 7nom EMDB-12507, PDB-7nom: Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 4'Beta 手法: EM (サブトモグラム平均) / 解像度: 6.7 Å 12508 7non EMDB-12508, PDB-7non: Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 4'Gamma 手法: EM (サブトモグラム平均) / 解像度: 6.8 Å 12509 7noo EMDB-12509, PDB-7noo: Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 5'Alpha 手法: EM (サブトモグラム平均) / 解像度: 7.9 Å 12510 7nop EMDB-12510, PDB-7nop: Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 5'Beta 手法: EM (サブトモグラム平均) / 解像度: 7.8 Å 12511 7noq EMDB-12511, PDB-7noq: Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 5'Gamma 手法: EM (サブトモグラム平均) / 解像度: 6.5 Å EMDB-12774: Cryo-electron tomogram of mature RSV CANC CLPs 手法: EM (トモグラフィー)
由来	rous sarcoma virus (strain prague c) (ラウス肉腫ウイルス) Rous sarcoma virus - Prague C (ラウス肉腫ウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / Retrovirus (レトロウイルス科) / Rous sarcoma virus (ラウス肉腫ウイルス) / capsid protein (カプシド) / IP6