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Structure paper

タイトル	The structure of the yeast mitochondrial ribosome.
ジャーナル・号・ページ	Science, Vol. 355, Issue 6324, Page 528-531, Year 2017
掲載日	2017年2月3日
著者	Nirupa Desai / Alan Brown / Alexey Amunts / V Ramakrishnan /
PubMed 要旨	Mitochondria have specialized ribosomes (mitoribosomes) dedicated to the expression of the genetic information encoded by their genomes. Here, using electron cryomicroscopy, we have determined the ...Mitochondria have specialized ribosomes (mitoribosomes) dedicated to the expression of the genetic information encoded by their genomes. Here, using electron cryomicroscopy, we have determined the structure of the 75-component yeast mitoribosome to an overall resolution of 3.3 angstroms. The mitoribosomal small subunit has been built de novo and includes 15S ribosomal RNA (rRNA) and 34 proteins, including 14 without homologs in the evolutionarily related bacterial ribosome. Yeast-specific rRNA and protein elements, including the acquisition of a putatively active enzyme, give the mitoribosome a distinct architecture compared to the mammalian mitoribosome. At an expanded messenger RNA channel exit, there is a binding platform for translational activators that regulate translation in yeast but not mammalian mitochondria. The structure provides insights into the evolution and species-specific specialization of mitochondrial translation.
リンク	Science / PubMed:28154081 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 4.97 Å
構造データ	3551 5mrc EMDB-3551, PDB-5mrc: Structure of the yeast mitochondrial ribosome - Class A 手法: EM (単粒子) / 解像度: 3.25 Å 3552 5mre EMDB-3552, PDB-5mre: Structure of the yeast mitochondrial ribosome - Class B 手法: EM (単粒子) / 解像度: 3.75 Å 3553 5mrf EMDB-3553, PDB-5mrf: Structure of the yeast mitochondrial ribosome - Class C 手法: EM (単粒子) / 解像度: 4.97 Å EMDB-3554: Yeast mitochondrial ribosome - small subunit head (fitted to EMD 3551) 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-3555: Yeast mitochondrial ribosome - small subunit body (fitted to EMD 3551) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-3556: Yeast mitochondrial ribosome - large subunit (fitted to EMD 3551) 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-ZN: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子*YM / グアノシン二リン酸
由来	saccharomyces cerevisiae (パン酵母) Baker's yeast (パン酵母)
キーワード	RIBOSOME (リボソーム) / yeast (酵母) / mitochondrial (ミトコンドリア) / saccharomyces cerevisiae (出芽酵母)