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TitleInsights into product binding in sucrose isomerases from crystal structures of MutB from Rhizobium sp.
Journal, issue, pagesTo be Published
Publish dateSep 20, 2012 (structure data deposition date)
AuthorsLipski, A. / Watzlawick, H. / Ravaud, S. / Robert, X. / Haser, R. / Mattes, R. / Aghajari, N.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.8 - 2.2 Å
Structure data

PDB-4h7v:
MUTB inactive double mutant D200A-D415N in complex with GLUCOSE
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-4h8h:
MUTB inactive double mutant E254Q-D415N
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-4h8u:
MUTB inactive double mutant D200A-D415N soaked with sucrose and having as bound ligands sucrose in molecule A and the reaction product trehalulose in molecule B
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-4h8v:
Crystal structure of the trehalulose synthase MUTB in complex with trehalulose
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-4ha1:
MutB inactive double mutant D200A-D415N in complex with isomaltulose
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-GLC:
alpha-D-glucopyranose / Glucose

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-HOH:
WATER / Water

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-TEU:
1-O-alpha-D-glucopyranosyl-D-fructose

ChemComp-ISL:
6-O-alpha-D-glucopyranosyl-D-fructose / Isomaltulose

Source
  • rhizobium (bacteria)
KeywordsISOMERASE / ISOMALTULOSE SYNTHASE LIKE / TIM-BARREL / (BETA/ALPHA)8 / SUCROSE ISOMERASE / TREHALULOSE SYNTHASE / GH13 FAMILY (CAZY DATABASE) / CALCIUM BINDING / GLYCOSIDE HYDROLASE / ISOMALTULOSE SYNTHASE LIKE INACTIVE MUTANT / NATIVE ENZYME

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