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Structure paper

TitleDe novo design of modular peptide-binding proteins by superhelical matching.
Journal, issue, pagesNature, Vol. 616, Page 581-589, Year 2023
Publish dateMar 20, 2022 (structure data deposition date)
AuthorsWu, K. / Bai, H. / Chang, Y.T. / Redler, R. / McNally, K.E. / Sheffler, W. / Brunette, T.J. / Hicks, D.R. / Morgan, T.E. / Stevens, T.J. ...Wu, K. / Bai, H. / Chang, Y.T. / Redler, R. / McNally, K.E. / Sheffler, W. / Brunette, T.J. / Hicks, D.R. / Morgan, T.E. / Stevens, T.J. / Broerman, A. / Goreshnik, I. / DeWitt, M. / Chow, C.M. / Shen, Y. / Stewart, L. / Derivery, E. / Silva, D.A. / Bhabha, G. / Ekiert, D.C. / Baker, D.
External linksNature / PubMed:37020023
MethodsX-ray diffraction
Resolution2.15 - 3.18 Å
Structure data

PDB-7udj:
Crystal structure of designed helical repeat protein RPB_PEW3_R4 bound to PAWx4 peptide
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-7udk:
Crystal structure of designed helical repeat protein RPB_LRP2_R4 bound to LRPx4 peptide
Method: X-RAY DIFFRACTION / Resolution: 3.18 Å

PDB-7udl:
Crystal structure of designed helical repeat protein RPB_PLP1_R6 bound to PLPx6 peptide
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-7udm:
Crystal structure of designed helical repeat protein RPB_PLP1_R6 in alternative conformation 1 (with peptide)
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-7udn:
Crystal structure of designed helical repeat protein RPB_PLP1_R6 in alternative conformation 2
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-7udo:
Crystal structure of designed helical repeat protein RPB_LRP2_R4 (proteolysis fragment?), forming pseudopolymeric filaments
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-7ue2:
Crystal structure of designed helical repeat protein RPB_PLP3_R6 bound to PLPx6 peptide
Method: X-RAY DIFFRACTION / Resolution: 2.68 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-HOH:
WATER / Water

ChemComp-PO4:
PHOSPHATE ION / Phosphate

Source
  • synthetic construct (others)
KeywordsDE NOVO PROTEIN / Synthetic protein / designed helical repeat protein / peptide binder / peptide binding / designed helical repeat (DHR)

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