EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular basis of eIF5A-dependent CAT tailing in eukaryotic ribosome-associated quality control.
ジャーナル・号・ページ	Mol Cell, Vol. 83, Issue 4, Page 607-621.e4, Year 2023
掲載日	2023年2月16日
著者	Petr Tesina / Shuhei Ebine / Robert Buschauer / Matthias Thoms / Yoshitaka Matsuo / Toshifumi Inada / Roland Beckmann /
PubMed 要旨	Ribosome-associated quality control (RQC) is a conserved process degrading potentially toxic truncated nascent peptides whose malfunction underlies neurodegeneration and proteostasis decline in aging. ...Ribosome-associated quality control (RQC) is a conserved process degrading potentially toxic truncated nascent peptides whose malfunction underlies neurodegeneration and proteostasis decline in aging. During RQC, dissociation of stalled ribosomes is followed by elongation of the nascent peptide with alanine and threonine residues, driven by Rqc2 independently of mRNA, the small ribosomal subunit and guanosine triphosphate (GTP)-hydrolyzing factors. The resulting CAT tails (carboxy-terminal tails) and ubiquitination by Ltn1 mark nascent peptides for proteasomal degradation. Here we present ten cryogenic electron microscopy (cryo-EM) structures, revealing the mechanistic basis of individual steps of the CAT tailing cycle covering initiation, decoding, peptidyl transfer, and tRNA translocation. We discovered eIF5A as a crucial eukaryotic RQC factor enabling peptidyl transfer. Moreover, we observed dynamic behavior of RQC factors and tRNAs allowing for processivity of the CAT tailing cycle without additional energy input. Together, these results elucidate key differences as well as common principles between CAT tailing and canonical translation.
リンク	Mol Cell / PubMed:36804914
手法	EM (単粒子)
解像度	2.4 - 2.9 Å
構造データ	15296 8aaf EMDB-15296, PDB-8aaf: Yeast RQC complex in state G 手法: EM (単粒子) / 解像度: 2.5 Å 15423 8agt EMDB-15423, PDB-8agt: Yeast RQC complex in state F 手法: EM (単粒子) / 解像度: 2.6 Å 15424 8agu EMDB-15424, PDB-8agu: Yeast RQC complex in state E 手法: EM (単粒子) / 解像度: 2.7 Å 15425 8agv EMDB-15425, PDB-8agv: Yeast RQC complex in state H 手法: EM (単粒子) / 解像度: 2.6 Å 15426 8agw EMDB-15426, PDB-8agw: Yeast RQC complex in state D 手法: EM (単粒子) / 解像度: 2.6 Å 15427 8agx EMDB-15427, PDB-8agx: Yeast RQC complex in state with the RING domain of Ltn1 in the IN position 手法: EM (単粒子) / 解像度: 2.4 Å 15428 8agz EMDB-15428, PDB-8agz: Yeast RQC complex in state with the RING domain of Ltn1 in the OUT position 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-15430: Yeast RQC complex in state A (pre-initiation) 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-15431: Yeast RQC complex in state B (A-site tRNA only) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-15432: Yeast RQC complex in state C 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-15433: Yeast RQC complex in state I (translocation with A-site and E-site tRNAs) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-15434: Yeast RQC complex in state J 手法: EM (単粒子) / 解像度: 2.8 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-SPD: SPERMIDINE / スペルミジン / スペルミジン
由来	saccharomyces cerevisiae (パン酵母) baker's yeast (パン酵母)
キーワード	TRANSLATION (翻訳 (生物学)) / ribosome-associated quality control / NEMF / Listerin / CAT tailing