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TitlePathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism.
Journal, issue, pagesSci Adv, Vol. 8, Issue 36, Page eabq5108, Year 2022
Publish dateSep 9, 2022
AuthorsYan-Bo Zhao / Meng-Xi Liu / Tao-Tao Chen / Xiaomin Ma / Ze-Kai Li / Zichao Zheng / Si-Ru Zheng / Lifei Chen / You-Zhi Li / Li-Rui Tang / Qi Chen / Peiyi Wang / Songying Ouyang /
PubMed AbstractNucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. The direct recognition mechanism of pathogen effectors by coiled-coil NLRs (CNLs) ...Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. The direct recognition mechanism of pathogen effectors by coiled-coil NLRs (CNLs) remains unclear. We demonstrate that the CNL Sr35 directly recognizes the pathogen effector AvrSr35 from f. sp and report a cryo-electron microscopy structure of Sr35 resistosome and a crystal structure of AvrSr35. We show that AvrSr35 forms homodimers that are disassociated into monomers upon direct recognition by the leucine-rich repeat domain of Sr35, which induces Sr35 resistosome assembly and the subsequent immune response. The first 20 amino-terminal residues of Sr35 are indispensable for immune signaling but not for plasma membrane association. Our findings reveal the direct recognition and activation mechanism of a plant CNL and provide insights into biochemical function of Sr35 resistosome.
External linksSci Adv / PubMed:36083908 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.06 - 3.6 Å
Structure data

33153

7xe0

EMDB-33153, PDB-7xe0:
Cryo-EM structure of plant NLR Sr35 resistosome
Method: EM (single particle) / Resolution: 3.33 Å

33486

7xvg

EMDB-33486, PDB-7xvg:
Cryo-EM structure of binary complex of plant NLR Sr35 and effector AvrSr35
Method: EM (single particle) / Resolution: 3.6 Å

33498

7xx2

EMDB-33498, PDB-7xx2:
Cryo-EM structure of Sr35 resistosome induced by AvrSr35 R381A
Method: EM (single particle) / Resolution: 3.6 Å

PDB-7xds:
Crystal structure of wheat stem rust effector AvrSr35
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

Chemicals

ChemComp-HOH:
WATER / Water

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Source
  • triticum monococcum (einkorn wheat)
  • puccinia graminis f. sp. tritici (fungus)
  • Puccinia graminis f.sp.tritici (fungus)
KeywordsPROTEIN TRANSPORT / Fungus / Effector / Wheat stem rust / PLANT PROTEIN / Plant immunity / NLR / resistosome

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