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Title | Translational termination without a stop codon. |
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Journal, issue, pages | Science, Vol. 354, Issue 6318, Page 1437-1440, Year 2016 |
Publish date | Dec 16, 2016 |
![]() | Nathan R James / Alan Brown / Yuliya Gordiyenko / V Ramakrishnan / ![]() |
PubMed Abstract | Ribosomes stall when they encounter the end of messenger RNA (mRNA) without an in-frame stop codon. In bacteria, these "nonstop" complexes can be rescued by alternative ribosome-rescue factor A (ArfA) ...Ribosomes stall when they encounter the end of messenger RNA (mRNA) without an in-frame stop codon. In bacteria, these "nonstop" complexes can be rescued by alternative ribosome-rescue factor A (ArfA). We used electron cryomicroscopy to determine structures of ArfA bound to the ribosome with 3'-truncated mRNA, at resolutions ranging from 3.0 to 3.4 angstroms. ArfA binds within the ribosomal mRNA channel and substitutes for the absent stop codon in the A site by specifically recruiting release factor 2 (RF2), initially in a compact preaccommodated state. A similar conformation of RF2 may occur on stop codons, suggesting a general mechanism for release-factor-mediated translational termination in which a conformational switch leads to peptide release only when the appropriate signal is present in the A site. |
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Methods | EM (single particle) |
Resolution | 2.97 - 3.35 Å |
Structure data | EMDB-3489, PDB-5mdv: EMDB-3490, PDB-5mdw: |
Chemicals | ![]() ChemComp-MG: ![]() ChemComp-FME: ![]() ChemComp-ZN: ![]() ChemComp-HOH: |
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