Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 113, Issue 40, Page 11232-11236, Year 2016
Publish date	Oct 4, 2016
Authors	Michael R Sawaya / Jose Rodriguez / Duilio Cascio / Michael J Collazo / Dan Shi / Francis E Reyes / Johan Hattne / Tamir Gonen / David S Eisenberg /
PubMed Abstract	Electrons, because of their strong interaction with matter, produce high-resolution diffraction patterns from tiny 3D crystals only a few hundred nanometers thick in a frozen-hydrated state. This ...Electrons, because of their strong interaction with matter, produce high-resolution diffraction patterns from tiny 3D crystals only a few hundred nanometers thick in a frozen-hydrated state. This discovery offers the prospect of facile structure determination of complex biological macromolecules, which cannot be coaxed to form crystals large enough for conventional crystallography or cannot easily be produced in sufficient quantities. Two potential obstacles stand in the way. The first is a phenomenon known as dynamical scattering, in which multiple scattering events scramble the recorded electron diffraction intensities so that they are no longer informative of the crystallized molecule. The second obstacle is the lack of a proven means of de novo phase determination, as is required if the molecule crystallized is insufficiently similar to one that has been previously determined. We show with four structures of the amyloid core of the Sup35 prion protein that, if the diffraction resolution is high enough, sufficiently accurate phases can be obtained by direct methods with the cryo-EM method microelectron diffraction (MicroED), just as in X-ray diffraction. The success of these four experiments dispels the concern that dynamical scattering is an obstacle to ab initio phasing by MicroED and suggests that structures of novel macromolecules can also be determined by direct methods.
External links	Proc Natl Acad Sci U S A / PubMed:27647903 / PubMed Central
Methods	EM (electron crystallography)
Resolution	1 - 1.1 Å
Structure data	8196 5k2e EMDB-8196, PDB-5k2e: Structure of NNQQNY from yeast prion Sup35 with zinc acetate determined by MicroED Method: EM (electron crystallography) 8197 5k2f EMDB-8197, PDB-5k2f: Structure of NNQQNY from yeast prion Sup35 with cadmium acetate determined by MicroED Method: EM (electron crystallography) 8198 5k2g EMDB-8198, PDB-5k2g: Structure of GNNQQNY from yeast prion Sup35 in space group P21 determined by MicroED Method: EM (electron crystallography) 8199 5k2h EMDB-8199, PDB-5k2h: Structure of GNNQQNY from yeast prion Sup35 in space group P212121 determined by MicroED Method: EM (electron crystallography)
Chemicals	ChemComp-ZN: Unknown entry ChemComp-ACY: ACETIC ACID / Acetic acid ChemComp-HOH: WATER / Water ChemComp-CD: Unknown entry ChemComp-ACT: ACETATE ION / Acetate
Source	saccharomyces cerevisiae (brewer's yeast)
Keywords	PROTEIN FIBRIL / amyloid / yeast prion