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TitleGlycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy.
Journal, issue, pagesNat Struct Mol Biol, Vol. 23, Issue 10, Page 899-905, Year 2016
Publish dateSep 12, 2016
AuthorsAlexandra C Walls / M Alejandra Tortorici / Brandon Frenz / Joost Snijder / Wentao Li / Félix A Rey / Frank DiMaio / Berend-Jan Bosch / David Veesler /
PubMed AbstractThe threat of a major coronavirus pandemic urges the development of strategies to combat these pathogens. Human coronavirus NL63 (HCoV-NL63) is an α-coronavirus that can cause severe lower- ...The threat of a major coronavirus pandemic urges the development of strategies to combat these pathogens. Human coronavirus NL63 (HCoV-NL63) is an α-coronavirus that can cause severe lower-respiratory-tract infections requiring hospitalization. We report here the 3.4-Å-resolution cryo-EM reconstruction of the HCoV-NL63 coronavirus spike glycoprotein trimer, which mediates entry into host cells and is the main target of neutralizing antibodies during infection. The map resolves the extensive glycan shield obstructing the protein surface and, in combination with mass spectrometry, provides a structural framework to understand the accessibility to antibodies. The structure reveals the complete architecture of the fusion machinery including the triggering loop and the C-terminal domains, which contribute to anchoring the trimer to the viral membrane. Our data further suggest that HCoV-NL63 and other coronaviruses use molecular trickery, based on epitope masking with glycans and activating conformational changes, to evade the immune system of infected hosts.
External linksNat Struct Mol Biol / PubMed:27617430 / PubMed Central
MethodsEM (single particle)
Resolution3.4 Å
Structure data

8331

5szs

EMDB-8331, PDB-5szs:
Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • human coronavirus nl63
KeywordsVIRAL PROTEIN / coronavirus / viral fusion protein / vaccine / NL63

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