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TitleStructure of AP205 Coat Protein Reveals Circular Permutation in ssRNA Bacteriophages.
Journal, issue, pagesJ Mol Biol, Vol. 428, Issue 21, Page 4267-4279, Year 2016
Publish dateOct 23, 2016
AuthorsMihails Shishovs / Janis Rumnieks / Christoph Diebolder / Kristaps Jaudzems / Loren B Andreas / Jan Stanek / Andris Kazaks / Svetlana Kotelovica / Inara Akopjana / Guido Pintacuda / Roman I Koning / Kaspars Tars /
PubMed AbstractAP205 is a single-stranded RNA bacteriophage that has a coat protein sequence not similar to any other known single-stranded RNA phage. Here, we report an atomic-resolution model of the AP205 virus- ...AP205 is a single-stranded RNA bacteriophage that has a coat protein sequence not similar to any other known single-stranded RNA phage. Here, we report an atomic-resolution model of the AP205 virus-like particle based on a crystal structure of an unassembled coat protein dimer and a cryo-electron microscopy reconstruction of the assembled particle, together with secondary structure information from site-specific solid-state NMR data. The AP205 coat protein dimer adopts the conserved Leviviridae coat protein fold except for the N-terminal region, which forms a beta-hairpin in the other known single-stranded RNA phages. AP205 has a similar structure at the same location formed by N- and C-terminal beta-strands, making it a circular permutant compared to the other coat proteins. The permutation moves the coat protein termini to the most surface-exposed part of the assembled particle, which explains its increased tolerance to long N- and C-terminal fusions.
External linksJ Mol Biol / PubMed:27591890
MethodsEM (single particle) / X-ray diffraction
Resolution1.73 - 6.0 Å
Structure data

4098

5lqp

EMDB-4098, PDB-5lqp:
Cryo-EM reconstruction of bacteriophage AP205 virus-like particles.
Method: EM (single particle) / Resolution: 6.0 Å

PDB-5fs4:
Bacteriophage AP205 coat protein
Method: X-RAY DIFFRACTION / Resolution: 1.73 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • acinetobacter phage ap205 (virus)
KeywordsVIRAL PROTEIN / SMALL RNA PHAGE / COAT PROTEIN / AP205 / VIRUS LIKE PARTICLE / RNA bacteriophage / Leviviridae / virus-like particle

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