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TitleVisualizing the molecular sociology at the HeLa cell nuclear periphery.
Journal, issue, pagesScience, Vol. 351, Issue 6276, Page 969-972, Year 2016
Publish dateFeb 26, 2016
AuthorsJulia Mahamid / Stefan Pfeffer / Miroslava Schaffer / Elizabeth Villa / Radostin Danev / Luis Kuhn Cuellar / Friedrich Förster / Anthony A Hyman / Jürgen M Plitzko / Wolfgang Baumeister /
PubMed AbstractThe molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo-electron tomography (cryo-ET) to produce three-dimensional snapshots ...The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo-electron tomography (cryo-ET) to produce three-dimensional snapshots of the HeLa cell nuclear periphery. Subtomogram averaging and classification of ribosomes revealed the native structure and organization of the cytoplasmic translation machinery. Analysis of a large dynamic structure-the nuclear pore complex-revealed variations detectable at the level of individual complexes. Cryo-ET was used to visualize previously elusive structures, such as nucleosome chains and the filaments of the nuclear lamina, in situ. Elucidation of the lamina structure provides insight into its contribution to metazoan nuclear stiffness.
External linksScience / PubMed:26917770
MethodsEM (tomography) / EM (subtomogram averaging)
Resolution28.0 - 92.0 Å
Structure data

EMDB-11992:
the molecular sociology at the HeLa cell nuclear periphery
Method: EM (tomography)

EMDB-8054:
In situ sub-tomogram average of HeLa nuclear pore complex from a single cell
Method: EM (subtomogram averaging) / Resolution: 77.0 Å

EMDB-8055:
In situ sub-tomogram average of HeLa nuclear pore complex
Method: EM (subtomogram averaging) / Resolution: 92.0 Å

EMDB-8056:
In situ sub-tomogram average of HeLa ER-associated ribosomes
Method: EM (subtomogram averaging) / Resolution: 35.0 Å

EMDB-8057:
In situ sub-tomogram average of HeLa cytosolic ribosomes
Method: EM (subtomogram averaging) / Resolution: 28.0 Å

Source
  • Homo sapiens (human)

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