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TitleStructure and function of outer dynein arm intermediate and light chain complex.
Journal, issue, pagesMol Biol Cell, Vol. 27, Issue 7, Page 1051-1059, Year 2016
Publish dateApr 1, 2016
AuthorsToshiyuki Oda / Tatsuki Abe / Haruaki Yanagisawa / Masahide Kikkawa /
PubMed AbstractThe outer dynein arm (ODA) is a molecular complex that drives the beating motion of cilia/flagella. Chlamydomonas ODA is composed of three heavy chains (HCs), two ICs, and 11 light chains (LCs). ...The outer dynein arm (ODA) is a molecular complex that drives the beating motion of cilia/flagella. Chlamydomonas ODA is composed of three heavy chains (HCs), two ICs, and 11 light chains (LCs). Although the three-dimensional (3D) structure of the whole ODA complex has been investigated, the 3D configurations of the ICs and LCs are largely unknown. Here we identified the 3D positions of the two ICs and three LCs using cryo-electron tomography and structural labeling. We found that these ICs and LCs were all localized at the root of the outer-inner dynein (OID) linker, designated the ODA-Beak complex. Of interest, the coiled-coil domain of IC2 extended from the ODA-Beak to the outer surface of ODA. Furthermore, we investigated the molecular mechanisms of how the OID linker transmits signals to the ODA-Beak, by manipulating the interaction within the OID linker using a chemically induced dimerization system. We showed that the cross-linking of the OID linker strongly suppresses flagellar motility in vivo. These results suggest that the ICs and LCs of the ODA form the ODA-Beak, which may be involved in mechanosignaling from the OID linker to the HCs.
External linksMol Biol Cell / PubMed:26864626 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution46.0 - 48.0 Å
Structure data

EMDB-6515:
Structure and function of outer dynein intermediate and light chain complex
Method: EM (subtomogram averaging) / Resolution: 47.0 Å

EMDB-6516:
Structure and function of outer dynein intermediate and light chain complex
Method: EM (subtomogram averaging) / Resolution: 46.0 Å

EMDB-6517:
Structure and function of outer dynein intermediate and light chain complex
Method: EM (subtomogram averaging) / Resolution: 48.0 Å

EMDB-6518:
Structure and function of outer dynein intermediate and light chain complex
Method: EM (subtomogram averaging) / Resolution: 48.0 Å

EMDB-6519:
Structure and function of outer dynein intermediate and light chain complex
Method: EM (subtomogram averaging) / Resolution: 47.0 Å

EMDB-6520:
Structure and function of outer dynein intermediate and light chain complex
Method: EM (subtomogram averaging) / Resolution: 48.0 Å

EMDB-6521:
Structure and function of outer dynein intermediate and light chain complex
Method: EM (subtomogram averaging) / Resolution: 46.0 Å

EMDB-6522:
Structure and function of outer dynein intermediate and light chain complex
Method: EM (subtomogram averaging) / Resolution: 46.0 Å

EMDB-6523:
Structure and function of outer dynein intermediate and light chain complex
Method: EM (subtomogram averaging) / Resolution: 47.0 Å

EMDB-6524:
Structure and function of outer dynein intermediate and light chain complex
Method: EM (subtomogram averaging) / Resolution: 47.0 Å

Source
  • Chlamydomonas reinhardtii (plant)

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