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TitleMolecular architecture of the ribosome-bound Hepatitis C Virus internal ribosomal entry site RNA.
Journal, issue, pagesEMBO J, Vol. 34, Issue 24, Page 3042-3058, Year 2015
Publish dateDec 14, 2015
AuthorsHiroshi Yamamoto / Marianne Collier / Justus Loerke / Jochen Ismer / Andrea Schmidt / Tarek Hilal / Thiemo Sprink / Kaori Yamamoto / Thorsten Mielke / Jörg Bürger / Tanvir R Shaikh / Marylena Dabrowski / Peter W Hildebrand / Patrick Scheerer / Christian M T Spahn /
PubMed AbstractInternal ribosomal entry sites (IRESs) are structured cis-acting RNAs that drive an alternative, cap-independent translation initiation pathway. They are used by many viruses to hijack the ...Internal ribosomal entry sites (IRESs) are structured cis-acting RNAs that drive an alternative, cap-independent translation initiation pathway. They are used by many viruses to hijack the translational machinery of the host cell. IRESs facilitate translation initiation by recruiting and actively manipulating the eukaryotic ribosome using only a subset of canonical initiation factor and IRES transacting factors. Here we present cryo-EM reconstructions of the ribosome 80S- and 40S-bound Hepatitis C Virus (HCV) IRES. The presence of four subpopulations for the 80S•HCV IRES complex reveals dynamic conformational modes of the complex. At a global resolution of 3.9 Å for the most stable complex, a derived atomic model reveals a complex fold of the IRES RNA and molecular details of its interaction with the ribosome. The comparison of obtained structures explains how a modular architecture facilitates mRNA loading and tRNA binding to the P-site. This information provides the structural foundation for understanding the mechanism of HCV IRES RNA-driven translation initiation.
External linksEMBO J / PubMed:26604301 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 6.7 Å
Structure data

3221

5flx

EMDB-3221: Mammalian 80S HCV-IRES complex, Classical
PDB-5flx: Mammalian 40S HCV-IRES complex
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-3223:
Mammalian 80S HCV-IRES complex, Classical / no head tilt
Method: EM (single particle) / Resolution: 5.8 Å

EMDB-3224:
Mammalian 40S HCV-IRES complex
Method: EM (single particle) / Resolution: 6.7 Å

EMDB-3225:
Mammalian 80S HCV-IRES complex, Rolled
Method: EM (single particle) / Resolution: 5.6 Å

EMDB-3226:
Mammalian 80S HCV-IRES complex, Rotated
Method: EM (single particle) / Resolution: 5.8 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • oryctolagus cuniculus (rabbit)
  • hepatitis c virus
KeywordsRIBOSOME / TRANSLATION INITIATION / HEPATITIS C VIRUS INTERNAL RIBOSOME ENTRY SITE

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