Gira Bhabha / Hui-Chun Cheng / Nan Zhang / Arne Moeller / Maofu Liao / Jeffrey A Speir / Yifan Cheng / Ronald D Vale /
PubMed Abstract
Dyneins power microtubule motility using ring-shaped, AAA-containing motor domains. Here, we report X-ray and electron microscopy (EM) structures of yeast dynein bound to different ATP analogs, which ...Dyneins power microtubule motility using ring-shaped, AAA-containing motor domains. Here, we report X-ray and electron microscopy (EM) structures of yeast dynein bound to different ATP analogs, which collectively provide insight into the roles of dynein's two major ATPase sites, AAA1 and AAA3, in the conformational change mechanism. ATP binding to AAA1 triggers a cascade of conformational changes that propagate to all six AAA domains and cause a large movement of the "linker," dynein's mechanical element. In contrast to the role of AAA1 in driving motility, nucleotide transitions in AAA3 gate the transmission of conformational changes between AAA1 and the linker, suggesting that AAA3 acts as a regulatory switch. Further structural and mutational studies also uncover a role for the linker in regulating the catalytic cycle of AAA1. Together, these results reveal how dynein's two major ATP-binding sites initiate and modulate conformational changes in the motor domain during motility.
EMDB-6047: Cryo-EM structure of the dynein motor domain in the presence of ADP-AlF3 Method: EM (single particle) / Resolution: 10.5 Å
EMDB-6048: Cryo-EM structure of the dynein motor domain in the presence of ADP-AlF3 Method: EM (single particle) / Resolution: 15.0 Å
EMDB-6049: Cryo-EM structure of the yeast dynein motor domain in the presence of ADP-vanadate Method: EM (single particle) / Resolution: 9.0 Å
EMDB-6050: Cryo-EM structure of the yeast dynein motor domain in the presence of ADP-vanadate Method: EM (single particle) / Resolution: 15.0 Å
EMDB-6051: Cryo-EM structure of the yeast dynein motor domain in the presence of ADP-vanadate Method: EM (single particle) / Resolution: 14.0 Å
EMDB-6052: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ADP-vanadate Method: EM (single particle) / Resolution: 24.0 Å
EMDB-6053: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ADP-vanadate Method: EM (single particle) / Resolution: 27.0 Å
EMDB-6054: Negative stain EM structure of the yeast dynein motor domain in the presence of ATP Method: EM (single particle) / Resolution: 17.0 Å
EMDB-6055: Negative stain EM structure of the yeast dynein motor domain in the presence of ATP Method: EM (single particle) / Resolution: 18.0 Å
EMDB-6056: Negative stain EM structure of the yeast dynein motor domain in the presence of ATP Method: EM (single particle) / Resolution: 15.0 Å
EMDB-6058: Negative stain EM structure of the yeast dynein motor domain in the presence of ATP Method: EM (single particle) / Resolution: 19.0 Å
EMDB-6059: Negative stain EM structure of the yeast dynein motor domain in the presence of ATP Method: EM (single particle) / Resolution: 16.0 Å
EMDB-6060: Negative stain EM structure of the apo yeast dynein motor domain Method: EM (single particle) / Resolution: 16.0 Å
EMDB-6061: Negative stain EM structure of the apo yeast dynein motor domain Method: EM (single particle) / Resolution: 25.0 Å
EMDB-6062: Negative stain EM structure of the yeast dynein motor domain in the presence of AMPPNP Method: EM (single particle) / Resolution: 15.0 Å
EMDB-6063: Negative stain EM structure of the yeast dynein motor domain in the presence of AMPPNP Method: EM (single particle) / Resolution: 15.0 Å
EMDB-6064: Negative stain EM structure of the yeast dynein motor domain in the presence of AMPPNP Method: EM (single particle) / Resolution: 15.0 Å
EMDB-6065: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ATP Method: EM (single particle) / Resolution: 23.0 Å
EMDB-6066: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ATP Method: EM (single particle) / Resolution: 21.0 Å
EMDB-6067: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ATP Method: EM (single particle) / Resolution: 23.0 Å
EMDB-6068: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ATP Method: EM (single particle) / Resolution: 23.0 Å
EMDB-6069: Negative stain EM structure of E2488Q mutant of the yeast dynein motor domain in the presence of ATP Method: EM (single particle) / Resolution: 20.0 Å
EMDB-6070: Negative stain EM structure of E1849Q/E2488Q mutant of the yeast dynein motor domain in the presence of ATP Method: EM (single particle) / Resolution: 22.0 Å
EMDB-6071: Negative stain EM structure of E1849Q/R2209A mutant of the yeast dynein motor domain in the presence of ATP Method: EM (single particle) / Resolution: 18.0 Å
EMDB-6072: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ADP-AlF3 Method: EM (single particle) / Resolution: 23.0 Å
EMDB-6073: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ADP-AlF3 Method: EM (single particle) / Resolution: 24.0 Å
EMDB-6074: Negative stain EM structure of the yeast dynein motor domain in the presence of ADP Method: EM (single particle) / Resolution: 15.0 Å
PDB-4w8f: Crystal structure of the dynein motor domain in the AMPPNP-bound state Method: X-RAY DIFFRACTION / Resolution: 3.541 Å
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MOTOR PROTEIN / 
