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TitleIncorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy.
Journal, issue, pagesNat Struct Biol, Vol. 10, Issue 11, Page 899-906, Year 2003
Publish dateOct 19, 2003
AuthorsMikel Valle / Andrey Zavialov / Wen Li / Scott M Stagg / Jayati Sengupta / Rikke C Nielsen / Poul Nissen / Stephen C Harvey / Måns Ehrenberg / Joachim Frank /
PubMed AbstractAminoacyl-tRNAs (aa-tRNAs) are delivered to the ribosome as part of the ternary complex of aa-tRNA, elongation factor Tu (EF-Tu) and GTP. Here, we present a cryo-electron microscopy (cryo-EM) study, ...Aminoacyl-tRNAs (aa-tRNAs) are delivered to the ribosome as part of the ternary complex of aa-tRNA, elongation factor Tu (EF-Tu) and GTP. Here, we present a cryo-electron microscopy (cryo-EM) study, at a resolution of approximately 9 A, showing that during the incorporation of the aa-tRNA into the 70S ribosome of Escherichia coli, the flexibility of aa-tRNA allows the initial codon recognition and its accommodation into the ribosomal A site. In addition, a conformational change observed in the GTPase-associated center (GAC) of the ribosomal 50S subunit may provide the mechanism by which the ribosome promotes a relative movement of the aa-tRNA with respect to EF-Tu. This relative rearrangement seems to facilitate codon recognition by the incoming aa-tRNA, and to provide the codon-anticodon recognition-dependent signal for the GTPase activity of EF-Tu. From these new findings we propose a mechanism that can explain the sequence of events during the decoding of mRNA on the ribosome.
External linksNat Struct Biol / PubMed:14566331
MethodsEM (single particle)
Resolution9.0 - 12.8 Å
Structure data

1055

1qza

1qzd

1r2x

EMDB-1055: Locking and unlocking of ribosomal motions.
PDB-1qza: Coordinates of the A/T site tRNA model fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin) bound 70S ribosome
PDB-1qzd: EF-Tu.kirromycin coordinates fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin) bound 70S ribosome
PDB-1r2x: Coordinates of L11 with 58nts of 23S rRNA fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin) bound 70S ribosome
Method: EM (single particle) / Resolution: 9.0 Å

1056

1qzb

1qzc

1r2w

EMDB-1056: Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy.
PDB-1qzb: Coordinates of the A-site tRNA model fitted into the cryo-EM map of 70S ribosome in the pre-translocational state
PDB-1qzc: Coordinates of S12, SH44, LH69 and SRL separately fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin) bound 70S ribosome
PDB-1r2w: Coordinates of L11 with 58nts of 23S rRNA fitted into the cryo-EM map of the 70S ribosome
Method: EM (single particle) / Resolution: 9.0 Å

EMDB-1395:
Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy.
Method: EM (single particle) / Resolution: 12.8 Å

Source
  • escherichia coli (E. coli)
  • thermus thermophilus (bacteria)
  • thermotoga maritima (bacteria)
KeywordsRNA / tRNA model / decoding / A/T-site tRNA. / A-site tRNA / RNA Binding Protein/RNA / ribosomal protein / rRNA / RNA Binding Protein-RNA COMPLEX / BIOSYNTHETIC PROTEIN / Elongation factor

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