Journal: Proc Natl Acad Sci U S A / Year: 2012 Title: Cryo-EM structure of a 3D DNA-origami object. Authors: Xiao-Chen Bai / Thomas G Martin / Sjors H W Scheres / Hendrik Dietz / Abstract: A key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has ...A key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has shown potential for creating user-defined 3D scaffolds, but the level of attainable positional accuracy has been unclear. Here we report the cryo-EM structure and a full pseudoatomic model of a discrete DNA object that is almost twice the size of a prokaryotic ribosome. The structure provides a variety of stable, previously undescribed DNA topologies for future use in nanotechnology and experimental evidence that discrete 3D DNA scaffolds allow the positioning of user-defined structural motifs with an accuracy that is similar to that observed in natural macromolecules. Thereby, our results indicate an attractive route to fabricate nanoscale devices that achieve complex functionalities by DNA-templated design steered by structural feedback.
DNA chain , 162 types, 162 molecules AAA0A1A2A3A4A5A6A7A8ABACADAEAFAGAHAIAJAKALAMANAOAPAQARASATAU...
#1: DNA chain
SCAFFOLDSTRAND,SCAFFOLDSTRAND
Mass: 2236529.500 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: ISOLATED M13 PHAGE GENOME (SINGLE-STRANDED),ISOLATED M13 PHAGE GENOME (SINGLE-STRANDED) Source: (gene. exp.) ENTEROBACTERIA PHAGE M13 (virus) Description: M13 SINGLE-STRANDED PHAGE GENOME WAS PRODUCED IN E.COLI CELLS Production host: ESCHERICHIA COLI (E. coli)
#2: DNA chain
STAPLESTRAND
Mass: 17040.047 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#3: DNA chain
STAPLESTRAND
Mass: 13578.773 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#4: DNA chain
STAPLESTRAND
Mass: 15511.108 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#5: DNA chain
STAPLESTRAND
Mass: 12230.890 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#6: DNA chain
STAPLESTRAND
Mass: 14807.590 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#7: DNA chain
STAPLESTRAND
Mass: 14780.572 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#8: DNA chain
STAPLESTRAND
Mass: 15464.999 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#9: DNA chain
STAPLESTRAND
Mass: 14646.427 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#10: DNA chain
STAPLESTRAND
Mass: 14915.617 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#11: DNA chain
STAPLESTRAND
Mass: 12142.799 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#12: DNA chain
STAPLESTRAND
Mass: 15050.683 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#13: DNA chain
STAPLESTRAND
Mass: 15408.909 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#14: DNA chain
STAPLESTRAND
Mass: 14141.102 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#15: DNA chain
STAPLESTRAND
Mass: 14669.425 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#16: DNA chain
STAPLESTRAND
Mass: 14206.161 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#17: DNA chain
STAPLESTRAND
Mass: 14627.406 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#18: DNA chain
STAPLESTRAND
Mass: 14811.582 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#19: DNA chain
STAPLESTRAND
Mass: 16067.389 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#20: DNA chain
STAPLESTRAND
Mass: 18479.871 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#21: DNA chain
STAPLESTRAND
Mass: 14739.470 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#22: DNA chain
STAPLESTRAND
Mass: 15255.813 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#23: DNA chain
STAPLESTRAND
Mass: 14723.457 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#24: DNA chain
STAPLESTRAND
Mass: 14647.427 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#25: DNA chain
STAPLESTRAND
Mass: 12225.881 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#26: DNA chain
STAPLESTRAND
Mass: 17719.348 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#27: DNA chain
STAPLESTRAND
Mass: 19372.441 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#28: DNA chain
STAPLESTRAND
Mass: 19698.684 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#29: DNA chain
STAPLESTRAND
Mass: 14866.562 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#30: DNA chain
STAPLESTRAND
Mass: 14811.582 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#31: DNA chain
STAPLESTRAND
Mass: 16044.354 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#32: DNA chain
STAPLESTRAND
Mass: 15186.788 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#33: DNA chain
STAPLESTRAND
Mass: 14668.473 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#34: DNA chain
STAPLESTRAND
Mass: 12847.249 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#35: DNA chain
STAPLESTRAND
Mass: 16569.660 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#36: DNA chain
STAPLESTRAND
Mass: 13844.921 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#37: DNA chain
STAPLESTRAND
Mass: 21594.908 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#38: DNA chain
STAPLESTRAND
Mass: 14677.449 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#39: DNA chain
STAPLESTRAND
Mass: 14724.548 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#40: DNA chain
STAPLESTRAND
Mass: 14873.547 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#41: DNA chain
STAPLESTRAND
Mass: 13352.602 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#42: DNA chain
STAPLESTRAND
Mass: 14051.038 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#43: DNA chain
STAPLESTRAND
Mass: 19167.404 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#44: DNA chain
STAPLESTRAND
Mass: 14427.287 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#45: DNA chain
STAPLESTRAND
Mass: 14603.398 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#46: DNA chain
STAPLESTRAND
Mass: 14738.465 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#47: DNA chain
STAPLESTRAND
Mass: 14783.497 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#48: DNA chain
STAPLESTRAND
Mass: 11088.116 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#49: DNA chain
STAPLESTRAND
Mass: 14856.577 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#50: DNA chain
STAPLESTRAND
Mass: 14668.435 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#51: DNA chain
STAPLESTRAND
Mass: 14776.514 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#52: DNA chain
STAPLESTRAND
Mass: 14700.488 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#53: DNA chain
STAPLESTRAND
Mass: 16015.279 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#54: DNA chain
STAPLESTRAND
Mass: 11672.545 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#55: DNA chain
STAPLESTRAND
Mass: 15777.127 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#56: DNA chain
STAPLESTRAND
Mass: 14858.482 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#57: DNA chain
STAPLESTRAND
Mass: 18217.744 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#58: DNA chain
STAPLESTRAND
Mass: 11130.170 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#59: DNA chain
STAPLESTRAND
Mass: 14792.514 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#60: DNA chain
STAPLESTRAND
Mass: 14648.434 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#61: DNA chain
STAPLESTRAND
Mass: 12362.992 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#62: DNA chain
STAPLESTRAND
Mass: 15522.011 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#63: DNA chain
STAPLESTRAND
Mass: 13550.692 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#64: DNA chain
STAPLESTRAND
Mass: 12030.770 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#65: DNA chain
STAPLESTRAND
Mass: 16897.852 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#66: DNA chain
STAPLESTRAND
Mass: 14819.556 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#67: DNA chain
STAPLESTRAND
Mass: 13567.786 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#68: DNA chain
STAPLESTRAND
Mass: 10961.058 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#69: DNA chain
STAPLESTRAND
Mass: 20973.498 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#70: DNA chain
STAPLESTRAND
Mass: 12298.959 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#71: DNA chain
STAPLESTRAND
Mass: 15221.823 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#72: DNA chain
STAPLESTRAND
Mass: 12828.285 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#73: DNA chain
STAPLESTRAND
Mass: 12823.292 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#74: DNA chain
STAPLESTRAND
Mass: 17870.438 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#75: DNA chain
STAPLESTRAND
Mass: 13573.818 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#76: DNA chain
STAPLESTRAND
Mass: 14710.436 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#77: DNA chain
STAPLESTRAND
Mass: 16003.303 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#78: DNA chain
STAPLESTRAND
Mass: 19274.367 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#79: DNA chain
STAPLESTRAND
Mass: 14948.607 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#80: DNA chain
STAPLESTRAND
Mass: 20124.887 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#81: DNA chain
STAPLESTRAND
Mass: 14687.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#82: DNA chain
STAPLESTRAND
Mass: 19689.631 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#83: DNA chain
STAPLESTRAND
Mass: 14721.426 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#84: DNA chain
STAPLESTRAND
Mass: 15960.209 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#85: DNA chain
STAPLESTRAND
Mass: 16950.857 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#86: DNA chain
STAPLESTRAND
Mass: 13490.700 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#87: DNA chain
STAPLESTRAND
Mass: 16259.448 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#88: DNA chain
STAPLESTRAND
Mass: 15029.691 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#89: DNA chain
STAPLESTRAND
Mass: 14731.495 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#90: DNA chain
STAPLESTRAND
Mass: 20185.957 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#91: DNA chain
STAPLESTRAND
Mass: 14797.471 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#92: DNA chain
STAPLESTRAND
Mass: 20881.359 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#93: DNA chain
STAPLESTRAND
Mass: 15441.856 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#94: DNA chain
STAPLESTRAND
Mass: 17205.010 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#95: DNA chain
STAPLESTRAND
Mass: 14825.497 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#96: DNA chain
STAPLESTRAND
Mass: 14900.536 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#97: DNA chain
STAPLESTRAND
Mass: 14388.232 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#98: DNA chain
STAPLESTRAND
Mass: 14887.535 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#99: DNA chain
STAPLESTRAND
Mass: 20567.201 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#100: DNA chain
STAPLESTRAND
Mass: 13759.860 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#101: DNA chain
STAPLESTRAND
Mass: 20663.260 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#102: DNA chain
STAPLESTRAND
Mass: 14911.580 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#103: DNA chain
STAPLESTRAND
Mass: 14672.440 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#104: DNA chain
STAPLESTRAND
Mass: 20798.240 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#105: DNA chain
STAPLESTRAND
Mass: 20573.143 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#106: DNA chain
STAPLESTRAND
Mass: 14902.566 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#107: DNA chain
STAPLESTRAND
Mass: 18002.525 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#108: DNA chain
STAPLESTRAND
Mass: 15694.010 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#109: DNA chain
STAPLESTRAND
Mass: 16673.688 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#110: DNA chain
STAPLESTRAND
Mass: 12603.098 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#111: DNA chain
STAPLESTRAND
Mass: 15650.125 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#112: DNA chain
STAPLESTRAND
Mass: 17173.100 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#113: DNA chain
STAPLESTRAND
Mass: 14633.459 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#114: DNA chain
STAPLESTRAND
Mass: 21768.979 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#115: DNA chain
STAPLESTRAND
Mass: 19270.379 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#116: DNA chain
STAPLESTRAND
Mass: 14873.601 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#117: DNA chain
STAPLESTRAND
Mass: 16051.352 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#118: DNA chain
STAPLESTRAND
Mass: 13617.758 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#119: DNA chain
STAPLESTRAND
Mass: 16552.635 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#120: DNA chain
STAPLESTRAND
Mass: 14576.394 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#121: DNA chain
STAPLESTRAND
Mass: 14929.622 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#122: DNA chain
STAPLESTRAND
Mass: 12401.041 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#123: DNA chain
STAPLESTRAND
Mass: 12350.960 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#124: DNA chain
STAPLESTRAND
Mass: 13706.768 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#125: DNA chain
STAPLESTRAND
Mass: 14949.592 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#126: DNA chain
STAPLESTRAND
Mass: 13565.741 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#127: DNA chain
STAPLESTRAND
Mass: 18067.650 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#128: DNA chain
STAPLESTRAND
Mass: 14922.639 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#129: DNA chain
STAPLESTRAND
Mass: 14789.549 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#130: DNA chain
STAPLESTRAND
Mass: 16638.734 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#131: DNA chain
STAPLESTRAND
Mass: 12745.254 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#132: DNA chain
STAPLESTRAND
Mass: 14860.582 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#133: DNA chain
STAPLESTRAND
Mass: 17217.082 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#134: DNA chain
STAPLESTRAND
Mass: 11567.484 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#135: DNA chain
STAPLESTRAND
Mass: 14660.464 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#136: DNA chain
STAPLESTRAND
Mass: 14767.515 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#137: DNA chain
STAPLESTRAND
Mass: 14951.619 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#138: DNA chain
STAPLESTRAND
Mass: 9754.358 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#139: DNA chain
STAPLESTRAND
Mass: 16271.479 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#140: DNA chain
STAPLESTRAND
Mass: 11708.513 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#141: DNA chain
STAPLESTRAND
Mass: 14417.234 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#142: DNA chain
STAPLESTRAND
Mass: 13206.617 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#143: DNA chain
STAPLESTRAND
Mass: 14907.681 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#144: DNA chain
STAPLESTRAND
Mass: 13626.824 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#145: DNA chain
STAPLESTRAND
Mass: 19047.244 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#146: DNA chain
STAPLESTRAND
Mass: 12966.436 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#147: DNA chain
STAPLESTRAND
Mass: 16020.326 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#148: DNA chain
STAPLESTRAND
Mass: 14770.532 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#149: DNA chain
STAPLESTRAND
Mass: 14218.170 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#150: DNA chain
STAPLESTRAND
Mass: 14330.200 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#151: DNA chain
STAPLESTRAND
Mass: 8874.730 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#152: DNA chain
STAPLESTRAND
Mass: 14865.575 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#153: DNA chain
STAPLESTRAND
Mass: 12440.024 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#154: DNA chain
STAPLESTRAND
Mass: 14115.087 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#155: DNA chain
STAPLESTRAND
Mass: 15330.904 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#156: DNA chain
STAPLESTRAND
Mass: 13488.727 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#157: DNA chain
STAPLESTRAND
Mass: 18509.984 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#158: DNA chain
STAPLESTRAND
Mass: 14077.056 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#159: DNA chain
STAPLESTRAND
Mass: 16704.740 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#160: DNA chain
STAPLESTRAND
Mass: 14153.132 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#161: DNA chain
STAPLESTRAND
Mass: 20481.178 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#162: DNA chain
STAPLESTRAND
Mass: 14727.513 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
-
Experimental details
-
Experiment
Experiment
Method: ELECTRON MICROSCOPY
EM experiment
Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction
-
Sample preparation
Component
Name: POINTER-SHAPED 3D DNA- ORIGAMI OBJECT / Type: COMPLEX
Buffer solution
Name: 10 MM TRIS-HCL, 20 MM MGCL2, 5 MM NACL, 1 MM EDTA / pH: 7.6 / Details: 10 MM TRIS-HCL, 20 MM MGCL2, 5 MM NACL, 1 MM EDTA
Specimen
Conc.: 48 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen support
Details: CARBON
Vitrification
Instrument: FEI VITROBOT MARK III / Cryogen name: ETHANE / Details: LIQUID ETHANE
-
Electron microscopy imaging
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company
Microscopy
Model: FEI POLARA 300 / Date: Apr 1, 2012
Electron gun
Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lens
Mode: BRIGHT FIELD / Nominal magnification: 31000 X / Calibrated magnification: 39436 X / Nominal defocus max: 4460 nm / Nominal defocus min: 890 nm / Cs: 2 mm
Specimen holder
Temperature: 80 K
Image recording
Electron dose: 10 e/Å2 / Film or detector model: FEI FALCON I (4k x 4k)
Image scans
Num. digital images: 283
Radiation wavelength
Relative weight: 1
-
Processing
EM software
ID
Name
Version
Category
1
CTFFIND
3
CTFcorrection
2
RELION
3Dreconstruction
CTF correction
Details: WIENER FILTER (RELION)
Symmetry
Point symmetry: C1 (asymmetric)
3D reconstruction
Method: MAXIMUM A POSTERIORI ESTIMATION / Resolution: 11.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 28502 / Nominal pixel size: 3.55 Å / Actual pixel size: 3.55 Å / Magnification calibration: EXPERIMENTAL CROSS-GRATING Details: TWO MODELS WERE REFINED INDEPENDENTLY TO PREVENT OVERFITTING THROUGH GOLD-STANDARD FOURIER-SHELL CORRELATIONS. THERE IS A MISSING COVALENT BOND BETWEEN THE LAST BASE OF THIS PDB FILE AND THE ...Details: TWO MODELS WERE REFINED INDEPENDENTLY TO PREVENT OVERFITTING THROUGH GOLD-STANDARD FOURIER-SHELL CORRELATIONS. THERE IS A MISSING COVALENT BOND BETWEEN THE LAST BASE OF THIS PDB FILE AND THE FIRST ONE IN PDB ENTRY 2YMG. IN ADDITION THERE IS A COVALENT BOND BETWEEN THE FIRST BASE IN THIS FILE AND THE LAST IN 2YMG GIVING A CIRCULAR MOLECULE. IN TOTAL THERE ARE FIVE PDB FILES. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2210.(DEPOSITION ID: 11146). Refinement type: HALF-MAPS REFINED INDEPENDENTLY / Symmetry type: POINT
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