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- EMDB-15651: Structure of the giant inhibitor of apoptosis, BIRC6 (multibody map 3) -

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Basic information

Entry
Database: EMDB / ID: EMD-15651
TitleStructure of the giant inhibitor of apoptosis, BIRC6 (multibody map 3)
Map dataMultibody map 3
Sample
  • Complex: Anti-parallel homodimer
    • Protein or peptide: BIRC6
KeywordsE2/E3 ubiquitin ligase / APOPTOSIS
Function / homology
Function and homology information


spongiotrophoblast layer development / labyrinthine layer development / ALK mutants bind TKIs / Flemming body / microtubule organizing center / cysteine-type endopeptidase inhibitor activity / ubiquitin conjugating enzyme activity / Signaling by ALK fusions and activated point mutants / regulation of cytokinesis / negative regulation of extrinsic apoptotic signaling pathway ...spongiotrophoblast layer development / labyrinthine layer development / ALK mutants bind TKIs / Flemming body / microtubule organizing center / cysteine-type endopeptidase inhibitor activity / ubiquitin conjugating enzyme activity / Signaling by ALK fusions and activated point mutants / regulation of cytokinesis / negative regulation of extrinsic apoptotic signaling pathway / RING-type E3 ubiquitin transferase / trans-Golgi network / spindle pole / ubiquitin-protein transferase activity / regulation of cell population proliferation / midbody / cell population proliferation / protein ubiquitination / endosome / cell cycle / cell division / protein phosphorylation / centrosome / apoptotic process / positive regulation of cell population proliferation / negative regulation of apoptotic process / membrane / nucleus / cytosol
Similarity search - Function
Baculoviral IAP repeat-containing protein 6 / Baculoviral IAP repeat-containing protein 6 / BIR repeat / Inhibitor of Apoptosis domain / BIR repeat profile. / Baculoviral inhibition of apoptosis protein repeat / Ubiquitin-conjugating enzyme E2, catalytic domain homologues / Ubiquitin-conjugating enzyme E2 / Ubiquitin-conjugating enzyme / Ubiquitin-conjugating (UBC) core domain profile. / Ubiquitin-conjugating enzyme/RWD-like
Similarity search - Domain/homology
Baculoviral IAP repeat-containing protein 6
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsDietz L / Elliott PR
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MR/R008582/1 United Kingdom
CitationJournal: Science / Year: 2023
Title: Structural basis for SMAC-mediated antagonism of caspase inhibition by the giant ubiquitin ligase BIRC6.
Authors: Larissa Dietz / Cara J Ellison / Carlos Riechmann / C Keith Cassidy / F Daniel Felfoldi / Adán Pinto-Fernández / Benedikt M Kessler / Paul R Elliott /
Abstract: Certain inhibitor of apoptosis (IAP) family members are sentinel proteins that prevent untimely cell death by inhibiting caspases. Antagonists, including second mitochondria-derived activator of ...Certain inhibitor of apoptosis (IAP) family members are sentinel proteins that prevent untimely cell death by inhibiting caspases. Antagonists, including second mitochondria-derived activator of caspases (SMAC), regulate IAPs and drive cell death. Baculoviral IAP repeat-containing protein 6 (BIRC6), a giant IAP with dual E2 and E3 ubiquitin ligase activity, regulates programmed cell death through unknown mechanisms. We show that BIRC6 directly restricts executioner caspase-3 and -7 and ubiquitinates caspase-3, -7, and -9, working exclusively with noncanonical E1, UBA6. Notably, we show that SMAC suppresses both mechanisms. Cryo-electron microscopy structures of BIRC6 alone and in complex with SMAC reveal that BIRC6 is an antiparallel dimer juxtaposing the substrate-binding module against the catalytic domain. Furthermore, we discover that SMAC multisite binding to BIRC6 results in a subnanomolar affinity interaction, enabling SMAC to competitively displace caspases, thus antagonizing BIRC6 anticaspase function.
History
DepositionAug 23, 2022-
Header (metadata) releaseMar 29, 2023-
Map releaseMar 29, 2023-
UpdateDec 13, 2023-
Current statusDec 13, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_15651.png

Downloads & links

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Map

FileDownload / File: emd_15651.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMultibody map 3
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.86 Å/pix.
x 300 pix.
= 258. Å		0.86 Å/pix.
x 300 pix.
= 258. Å		0.86 Å/pix.
x 300 pix.
= 258. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.86 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.019
Minimum - Maximum-0.076198146 - 0.1387589
Average (Standard dev.)-0.00016249 (±0.0038874797)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 258.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_15651_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: 2nd half map multibody map 3

Fileemd_15651_half_map_1.map
Annotation2nd half map multibody map 3
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: 1st half map multibody map 3

Fileemd_15651_half_map_2.map
Annotation1st half map multibody map 3
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Anti-parallel homodimer

EntireName: Anti-parallel homodimer
Components
  • Complex: Anti-parallel homodimer
    • Protein or peptide: BIRC6

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Supramolecule #1: Anti-parallel homodimer

SupramoleculeName: Anti-parallel homodimer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 1 MDa

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Macromolecule #1: BIRC6

MacromoleculeName: BIRC6 / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
Source (natural)Organism: Homo sapiens (human)
SequenceString: GPMVTGGGAA PPGTVTEPLP SVIVLSAGRK MAAAAAAASG PGCSSAAGAG AAGVSEWLVL RDGCMHCDAD GLHSLSYHPA LNAILAVTSR GTIKVIDGTS GATLQASALS AKPGGQVKCQ YISAVDKVIF VDDYAVGCRK DLNGILLLDT ALQTPVSKQD DVVQLELPVT ...String:
GPMVTGGGAA PPGTVTEPLP SVIVLSAGRK MAAAAAAASG PGCSSAAGAG AAGVSEWLVL RDGCMHCDAD GLHSLSYHPA LNAILAVTSR GTIKVIDGTS GATLQASALS AKPGGQVKCQ YISAVDKVIF VDDYAVGCRK DLNGILLLDT ALQTPVSKQD DVVQLELPVT EAQQLLSACL EKVDISSTEG YDLFITQLKD GLKNTSHETA ANHKVAKWAT VTFHLPHHVL KSIASAIVNE LKKINQNVAA LPVASSVMDR LSYLLPSARP ELGVGPGRSV DRSLMYSEAN RRETFTSWPH VGYRWAQPDP MAQAGFYHQP ASSGDDRAMC FTCSVCLVCW EPTDEPWSEH ERHSPNCPFV KGEHTQNVPL SVTLATSPAQ FPCTDGTDRI SCFGSGSCPH FLAAATKRGK ICIWDVSKLM KVHLKFEINA YDPAIVQQLI LSGDPSSGVD SRRPTLAWLE DSSSCSDIPK LEGDSDDLLE DSDSEEHSRS DSVTGHTSQK EAMEVSLDIT ALSILQQPEK LQWEIVANVL EDTVKDLEEL GANPCLTNSK SEKTKEKHQE QHNIPFPCLL AGGLLTYKSP ATSPISSNSH RSLDGLSRTQ GESISEQGST DNESCTNSEL NSPLVRRTLP VLLLYSIKES DEKAGKIFSQ MNNIMSKSLH DDGFTVPQII EMELDSQEQL LLQDPPVTYI QQFADAAANL TSPDSEKWNS VFPKPGTLVQ CLRLPKFAEE ENLCIDSITP CADGIHLLVG LRTCPVESLS AINQVEALNN LNKLNSALCN RRKGELESNL AVVNGANISV IQHESPADVQ TPLIIQPEQR NVSGGYLVLY KMNYATRIVT LEEEPIKIQH IKDPQDTITS LILLPPDILD NREDDCEEPI EDMQLTSKNG FEREKTSDIS TLGHLVITTQ GGYVKILDLS NFEILAKVEP PKKEGTEEQD TFVSVIYCSG TDRLCACTKG GELHFLQIGG TCDDIDEADI LVDGSLSKGI EPSSEGSKPL SNPSSPGISG VDLLVDQPFT LEILTSLVEL TRFETLTPRF SATVPPCWVE VQQEQQQRRH PQHLHQQHHG DAAQHTRTWK LQTDSNSWDE HVFELVLPKA CMVGHVDFKF VLNSNITNIP QIQVTLLKNK APGLGKVNAL NIEVEQNGKP SLVDLNEEMQ HMDVEESQCL RLCPFLEDHK EDILCGPVWL ASGLDLSGHA GMLTLTSPKL VKGMAGGKYR SFLIHVKAVN ERGTEEICNG GMRPVVRLPS LKHQSNKGYS LASLLAKVAA GKEKSSNVKN ENTSGTRKSE NLRGCDLLQE VSVTIRRFKK TSISKERVQR CAMLQFSEFH EKLLNTLCRK TDDGQITEHA QSLVLDTLCW LAGVHSNGPG SSKEGNENLL SKTRKFLSDI VRVCFFEAGR SIAHKCARFL ALCISNGKCD PCQPAFGPVL LKALLDNMSF LPAATTGGSV YWYFVLLNYV KDEDLAGCST ACASLLTAVS RQLQDRLTPM EALLQTRYGL YSSPFDPVLF DLEMSGSSCK NVYNSSIGVQ SDEIDLSDVL SGNGKVSSCT AAEGSFTSLT GLLEVEPLHF TCVSTSDGTR IERDDAMSSF GVTPAVGGLS SGTVGEASTA LSSAAQVALQ SLSHAMASAE QQLQVLQEKQ QQLLKLQQQK AKLEAKLHQT TAAAAAAASA VGPVHNSVPS NPVAAPGFFI HPSDVIPPTP KTTPLFMTPP LTPPNEAVSV VINAELAQLF PGSVIDPPAV NLAAHNKNSN KSRMNPLGSG LALAISHASH FLQPPPHQSI IIERMHSGAR RFVTLDFGRP ILLTDVLIPT CGDLASLSID IWTLGEEVDG RRLVVATDIS THSLILHDLI PPPVCRFMKI TVIGRYGSTN ARAKIPLGFY YGHTYILPWE SELKLMHDPL KGEGESANQP EIDQHLAMMV ALQEDIQCRY NLACHRLETL LQSIDLPPLN SANNAQYFLR KPDKAVEEDS RVFSAYQDCI QLQLQLNLAH NAVQRLKVAL GASRKMLSET SNPEDLIQTS STEQLRTIIR YLLDTLLSLL HASNGHSVPA VLQSTFHAQA CEELFKHLCI SGTPKIRLHT GLLLVQLCGG ERWWGQFLSN VLQELYNSEQ LLIFPQDRVF MLLSCIGQRS LSNSGVLESL LNLLDNLLSP LQPQLPMHRR TEGVLDIPMI SWVVMLVSRL LDYVATVEDE AAAAKKPLNG NQWSFINNNL HTQSLNRSSK GSSSLDRLYS RKIRKQLVHH KQQLNLLKAK QKALVEQMEK EKIQSNKGSS YKLLVEQAKL KQATSKHFKD LIRLRRTAEW SRSNLDTEVT TAKESPEIEP LPFTLAHERC ISVVQKLVLF LLSMDFTCHA DLLLFVCKVL ARIANATRPT IHLCEIVNEP QLERLLLLLV GTDFNRGDIS WGGAWAQYSL TCMLQDILAG ELLAPVAAEA MEEGTVGDDV GATAGDSDDS LQQSSVQLLE TIDEPLTHDI TGAPPLSSLE KDKEIDLELL QDLMEVDIDP LDIDLEKDPL AAKVFKPISS TWYDYWGADY GTYNYNPYIG GLGIPVAKPP ANTEKNGSQT VSVSVSQALD ARLEVGLEQQ AELMLKMMST LEADSILQAL TNTSPTLSQS PTGTDDSLLG GLQAANQTSQ LIIQLSSVPM LNVCFNKLFS MLQVHHVQLE SLLQLWLTLS LNSSSTGNKE NGADIFLYNA NRIPVISLNQ ASITSFLTVL AWYPNTLLRT WCLVLHSLTL MTNMQLNSGS SSAIGTQEST AHLLVSDPNL IHVLVKFLSG TSPHGTNQHS PQVGPTATQA MQEFLTRLQV HLSSTCPQIF SEFLLKLIHI LSTERGAFQT GQGPLDAQVK LLEFTLEQNF EVVSVSTISA VIESVTFLVH HYITCSDKVM SRSGSDSSVG ARACFGGLFA NLIRPGDAKA VCGEMTRDQL MFDLLKLVNI LVQLPLSGNR EYSARVSVTT NTTDSVSDEE KVSGGKDGNG SSTSVQGSPA YVADLVLANQ QIMSQILSAL GLCNSSAMAM IIGASGLHLT KHENFHGGLD AISVGDGLFT ILTTLSKKAS TVHMMLQPIL TYMACGYMGR QGSLATCQLS EPLLWFILRV LDTSDALKAF HDMGGVQLIC NNMVTSTRAI VNTARSMVST IMKFLDSGPN KAVDSTLKTR ILASEPDNAE GIHNFAPLGT ITSSSPTAQP AEVLLQATPP HRRARSAAWS YIFLPEEAWC DLTIHLPAAV LLKEIHIQPH LASLATCPSS VSVEVSADGV NMLPLSTPVV TSGLTYIKIQ LVKAEVASAV CLRLHRPRDA STLGLSQIKL LGLTAFGTTS SATVNNPFLP SEDQVSKTSI GWLRLLHHCL THISDLEGMM ASAAAPTANL LQTCAALLMS PYCGMHSPNI EVVLVKIGLQ STRIGLKLID ILLRNCAASG SDPTDLNSPL LFGRLNGLSS DSTIDILYQL GTTQDPGTKD RIQALLKWVS DSARVAAMKR SGRMNYMCPN SSTVEYGLLM PSPSHLHCVA AILWHSYELL VEYDLPALLD QELFELLFNW SMSLPCNMVL KKAVDSLLCS MCHVHPNYFS LLMGWMGITP PPVQCHHRLS MTDDSKKQDL SSSLTDDSKN AQAPLALTES HLATLASSSQ SPEAIKQLLD SGLPSLLVRS LASFCFSHIS SSESIAQSID ISQDKLRRHH VPQQCNKMPI TADLVAPILR FLTEVGNSHI MKDWLGGSEV NPLWTALLFL LCHSGSTSGS HNLGAQQTSA RSASLSSAAT TGLTTQQRTA IENATVAFFL QCISCHPNNQ KLMAQVLCEL FQTSPQRGNL PTSGNISGFI RRLFLQLMLE DEKVTMFLQS PCPLYKGRIN ATSHVIQHPM YGAGHKFRTL HLPVSTTLSD VLDRVSDTPS ITAKLISEQK DDKEKKNHEE KEKVKAENGF QDNYSVVVAS GLKSQSKRAV SATPPRPPSR RGRTIPDKIG STSGAEAANK IITVPVFHLF HKLLAGQPLP AEMTLAQLLT LLYDRKLPQG YRSIDLTVKL GSRVITDPSL SKTDSYKRLH PEKDHGDLLA SCPEDEALTP GDECMDGILD ESLLETCPIQ SPLQVFAGMG GLALIAERLP MLYPEVIQQV SAPVVTSTTQ EKPKDSDQFE WVTIEQSGEL VYEAPETVAA EPPPIKSAVQ TMSPIPAHSL AAFGLFLRLP GYAEVLLKER KHAQCLLRLV LGVTDDGEGS HILQSPSANV LPTLPFHVLR SLFSTTPLTT DDGVLLRRMA LEIGALHLIL VCLSALSHHS PRVPNSSVNQ TEPQVSSSHN PTSTEEQQLY WAKGTGFGTG STASGWDVEQ ALTKQRLEEE HVTCLLQVLA SYINPVSSAV NGEAQSSHET RGQNSNALPS VLLELLSQSC LIPAMSSYLR NDSVLDMARH VPLYRALLEL LRAIASCAAM VPLLLPLSTE NGEEEEEQSE CQTSVGTLLA KMKTCVDTYT NRLRSKRENV KTGVKPDASD QEPEGLTLLV PDIQKTAEIV YAATTSLRQA NQEKKLGEYS KKAAMKPKPL SVLKSLEEKY VAVMKKLQFD TFEMVSEDED GKLGFKVNYH YMSQVKNAND ANSAARARRL AQEAVTLSTS LPLSSSSSVF VRCDEERLDI MKVLITGPAD TPYANGCFEF DVYFPQDYPS SPPLVNLETT GGHSVRFNPN LYNDGKVCLS ILNTWHGRPE EKWNPQTSSF LQVLVSVQSL ILVAEPYFNE PGYERSRGTP SGTQSSREYD GNIRQATVKW AMLEQIRNPS PCFKEVIHKH FYLKRVEIMA QCEEWIADIQ QYSSDKRVGR TMSHHAAALK RHTAQLREEL LKLPCPEGLD PDTDDAPEVC RATTGAEETL MHDQVKPSSS KELPSDFQL

UniProtKB: Baculoviral IAP repeat-containing protein 6

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration4 mg/mL
BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK III

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Calibrated defocus max: 2.5 µm / Calibrated defocus min: 1.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 105000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average exposure time: 3.5 sec. / Average electron dose: 49.9 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 548509
Startup modelType of model: NONE
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 127710

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: OTHER

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