EMDB-11846: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.

Basic information

• Entry: Database: EMDB / ID: EMD-11846
• Title: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.

Sample

• Complex: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.
  • Complex: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.
    • Protein or peptide: x 53 types
    • RNA: x 1 types
  • Complex: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.
    • Protein or peptide: x 2 types
• Ligand: x 4 types

Function / homology

Function:

3-hydroxyisobutyryl-CoA hydrolase activity / TRAPP complex / Golgi vesicle transport / superoxide dismutase / superoxide dismutase activity / membrane => GO:0016020 / rRNA binding / ribosome / structural constituent of ribosome / translation / Golgi apparatus / endoplasmic reticulum / mitochondrion / metal ion binding

Domain/homology:

Ribosomal protein S12 / Ribosomal protein S12 / Nucleoid-associated protein YbaB-like domain superfamily / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / Probable Zinc-ribbon domain / Probable Zinc-ribbon domain / TRAPP I complex, subunit 5 / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / IGR protein motif / IGR / Cyclic phosphodiesterase / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / LysM domain superfamily / LysM domain / Rhodanese domain profile. / Rhodanese-like domain superfamily / LysM domain / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Rhodanese-like domain / Manganese/iron superoxide dismutase, N-terminal domain superfamily / ClpP/crotonase-like domain superfamily / Ribosomal protein S16 domain superfamily / Ribosomal protein S6 superfamily / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 superfamily / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S5, C-terminal / Ribosomal protein S8 / Ribosomal protein S5, C-terminal domain / Ribosomal protein S9 / Ribosomal protein S17/S11 / Ribosomal protein S9/S16 / Ribosomal protein S17 / Ribosomal protein S11 superfamily / Tetratricopeptide-like helical domain superfamily / S15/NS1, RNA-binding / Ribosomal protein S5 domain 2-type fold, subgroup / Ubiquitin-like domain superfamily / Ribosomal protein S5 domain 2-type fold / Nucleic acid-binding, OB-fold

Component:

Ribosome protein S12 / 30S Ribosomal protein S17-like protein / Superoxide dismutase, putative / Sod_Fe_C domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / AKAP7_NLS domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Rhodanese domain-containing protein / Uncharacterized protein / Ribosomal_S5_C domain-containing protein / Uncharacterized protein / Protein FYV4, mitochondrial / Uncharacterized protein / Uncharacterized protein / Trafficking protein particle complex subunit / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein

• Biological species: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Trypanosoma cruzi (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 3.5 Å
• Authors: Soufari H / Waltz F / Parrot C / Hashem Y

Funding support

France, 1 items

Organization	Grant number	Country
European Research Council (ERC)		France

• Citation: Journal: Proc Natl Acad Sci U S A / Year: 2020; Title: Structure of the mature kinetoplastids mitoribosome and insights into its large subunit biogenesis.; Authors: Heddy Soufari / Florent Waltz / Camila Parrot / Stéphanie Durrieu-Gaillard / Anthony Bochler / Lauriane Kuhn / Marie Sissler / Yaser Hashem / ; Abstract: Kinetoplastids are unicellular eukaryotic parasites responsible for such human pathologies as Chagas disease, sleeping sickness, and leishmaniasis. They have a single large mitochondrion, essential for the parasite survival. In kinetoplastid mitochondria, most of the molecular machineries and gene expression processes have significantly diverged and specialized, with an extreme example being their mitochondrial ribosomes. These large complexes are in charge of translating the few essential mRNAs encoded by mitochondrial genomes. Structural studies performed in already highlighted the numerous peculiarities of these mitoribosomes and the maturation of their small subunit. However, several important aspects mainly related to the large subunit (LSU) remain elusive, such as the structure and maturation of its ribosomal RNA. Here we present a cryo-electron microscopy study of the protozoans and mitoribosomes. For both species, we obtained the structure of their mature mitoribosomes, complete rRNA of the LSU, as well as previously unidentified ribosomal proteins. In addition, we introduce the structure of an LSU assembly intermediate in the presence of 16 identified maturation factors. These maturation factors act on both the intersubunit and the solvent sides of the LSU, where they refold and chemically modify the rRNA and prevent early translation before full maturation of the LSU.

History

Deposition	Oct 15, 2020	-
Header (metadata) release	Apr 28, 2021	-
Map release	Apr 28, 2021	-
Update	Jun 23, 2021	-
Current status	Jun 23, 2021	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_11846.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.19 Å

Density

Contour Level	By AUTHOR: 0.04 / Movie #1: 0.04
Minimum - Maximum	-0.1758366 - 0.30608553
Average (Standard dev.)	-0.000117868476 (±0.011968447)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 428.40002 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.19	1.19	1.19
M x/y/z	360	360	360
origin x/y/z	0.000	0.000	0.000
length x/y/z	428.400	428.400	428.400
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	72	72	65
NX/NY/NZ	157	157	169
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	360	360	360
D min/max/mean	-0.176	0.306	-0.000

Supplemental data

Sample components

Entire : mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.

• Entire: Name: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.

Components

• Complex: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.
  • Complex: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.
    • Protein or peptide: mS35
    • Protein or peptide: mS33
    • Protein or peptide: mS29
    • Protein or peptide: uS19
    • Protein or peptide: uS14m
    • Protein or peptide: uS10m
    • Protein or peptide: mS72
    • Protein or peptide: mS71
    • Protein or peptide: mS70
    • Protein or peptide: mS69
    • Protein or peptide: Rhodanese domain-containing protein
    • Protein or peptide: mS58
    • Protein or peptide: mS57
    • Protein or peptide: mS55
    • Protein or peptide: mS54
    • Protein or peptide: mS53
    • Protein or peptide: mS52
    • Protein or peptide: mS50
    • Protein or peptide: mS49
    • Protein or peptide: mS51
    • Protein or peptide: bS18m
    • Protein or peptide: uS15m
    • Protein or peptide: uS11m
    • Protein or peptide: uS8m
    • Protein or peptide: uS9m
    • Protein or peptide: mS73
    • Protein or peptide: mS68
    • Protein or peptide: mS66
    • Protein or peptide: AKAP7_NLS domain-containing protein
    • Protein or peptide: mS63
    • Protein or peptide: mS62
    • Protein or peptide: mS61
    • Protein or peptide: mS56
    • Protein or peptide: mS60
    • Protein or peptide: mS47
    • Protein or peptide: Sod_Fe_C domain-containing protein
    • Protein or peptide: Superoxide dismutase, putative
    • Protein or peptide: Protein FYV4, mitochondrial
    • Protein or peptide: mS37
    • Protein or peptide: mS38
    • Protein or peptide: Trafficking protein particle complex subunit
    • Protein or peptide: mS59
    • Protein or peptide: mS26
    • Protein or peptide: mS23
    • Protein or peptide: mS22
    • Protein or peptide: bS21m
    • Protein or peptide: bS16m
    • Protein or peptide: bS6m
    • Protein or peptide: MURF5
    • Protein or peptide: Ribosomal_S5_C domain-containing protein
    • Protein or peptide: mt-iF3
    • Protein or peptide: mS48
    • Protein or peptide: mS65
    • RNA: RNA (478-MER)
  • Complex: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.
    • Protein or peptide: 30S Ribosomal protein S17-like proteinRibosome
    • Protein or peptide: Ribosome protein S12
• Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
• Ligand: MAGNESIUM ION
• Ligand: URIDINE 5'-TRIPHOSPHATE
• Ligand: ZINC ION

Supramolecule #1: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.

• Supramolecule: Name: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#56

Supramolecule #2: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.

• Supramolecule: Name: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.; type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#52, #55-#56
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote)

Supramolecule #3: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.

• Supramolecule: Name: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.; type: complex / ID: 3 / Parent: 1 / Macromolecule list: #53-#54
• Source (natural): Organism: Trypanosoma cruzi (eukaryote)

Macromolecule #1: mS35

• Macromolecule: Name: mS35 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 99.238445 KDa

Sequence

String:

MLRARRPLPL LPGSRAWLLR FYSSTRELCQ LQSRERLILG THGAGVVQHA SVSQPLSVNF QSSVTVAAPA ADLFRTKVHE GTGTSGKDP YLRTLPNQES REPESSVSQA HITVAPTVDE CSTLDRRWES MQYWFNDQHP RLVIYLRQLQ VQDVPPISPA A ESLLSKFE EVAIPKLALD DTDRQRLTKL WGNLTEEAKA LRLHYVFDRF AFESKLSQLC KEALEQMHAM SLSGTEGSLA VE ALRRLTI LKRNDYIQRH LIDVTSNGAY LGFGDAVWRV FFSAVEAHKA VLFGKGTPDT IRFAWESILQ EDVVRVPDVT APV ALFLTL VCIHEGNRLA SVEWRESSSS LDEGICSSDN TQQRPLLALL NPVVKRRFVT KMVEFLLRSH SSNEFSKLLR KHGL HDLSR DVALCEAMNG SQGILEDDVA ELVSRFESTG EVKTLLSSLI GGKDAAVRET VAKIMGIPLA TTVDWDAMMQ SVDWT NNWR RLATKLLCDQ TLLVSIHKLV KNAIGAKGIS RHLFSEEYAD QLQSIIAIRE ERELNRKLKI DRIVRELSSY QRVDQS CEM LRQLGVDMKE LDQAALSIRQ DGLVKRPSVD ENVISRALEA VGNRHPNWVR AGVIAPGAIK DSIGALKAML FIFIRLS YV PQTGLAAMAQ RFRRRIGPIG VESFQFNIPT EVGFVEHYNN LEYKRYDWQG WYQRMVDVHN RNISLRCRVS DLKRLDPN G VPFVDMQTER RLRILAEGRV GMGVLMLDSD KYEDQKDNMT FGSIKLSELL SDARKAQLGE EYWPSVEMKV RKPSGQSKA HYSLIDYDRI EKKSRELYEK YRDAKKKSLF VTPMDLWLEV KGMQVRKASD GADADGYTVD ILQDALSSED NEKH

Macromolecule #2: mS33

• Macromolecule: Name: mS33 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 20.964881 KDa

Sequence

String:

MALRGVPIRL GVHRVGYTHP STLPVPCAQR WDLRLARARI FQEYIEEKAP GAWQLEDERS MSPEFKTFTG YPMREMRPGY GQNLPDFIM KKRLPNNTHY ELFARRDIPN EDNAMYGKYL YDMTVHGTSL PSTYRMHKDI NKAQRNDRKL SGNRFRVLCS S GAKKPPSG WEPIPDATEE EE

Macromolecule #3: mS29

• Macromolecule: Name: mS29 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 57.865008 KDa

Sequence

String:

MRSRLVGKSL ASLYVRPPVT CYTDACEAPV AMWNGAIPLK EVRVMQKGVP VRYVTKLYSH PLEASPTRLS FNDINSMYCV GNDELMQFF PEGLGGKVMQ LMPPGHPRGF LYRKEAHLLN LFIDKIQHWQ AKRNVLSSLT NNRPGFIIDG PKGCGKSALM C QVVHYARS RNLLTLYVPN AKEWTHGEWC WPSTILPGFF DAPDAARFFL RYFAKANRST LLSWRLKCTP NDLPVEQGER QP QNLYELC EWGHQVVAPA SIDRQSVCVK FLMDELSAEK KLPIVIVVDG WNLFSHDTHF RYPHPDFLRT LASLNDDSTD IDL YPQELP RIPASRLGFV RGLNKMILSK DEPNKFFFTC TTRDFKPFDG ISGFPDVETD RFTNSLDEYA PYDAEKDSLF HPIQ LGNFD EYEFRAFTRF LVNSGELAGL GWGPLWHFSS DFERKLYKIG FLSNRNPQGV IDHYHQELVW RYEYQRTRQK QYLLH RNME LVVSKRKNRH AEPKGG

Macromolecule #4: uS19

• Macromolecule: Name: uS19 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 19.86625 KDa

Sequence

String:

MLRRCCPATL HVAPSTAMVA GVFVNNQKRF LKMAKSAFGF YLARRGQRKF PFLRRPHIKN THAMNLSAPY FWSFMTAKSQ TYFLPEENY ITGDWTGKFF VSKLQVYTLQ HATSGSTVRV KSFPSVFELS SPSRWNIGKE LNTLTKPRMD LIDEQMLTKK Q RLDYVKAG LLPK

Macromolecule #5: uS14m

• Macromolecule: Name: uS14m / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 19.80592 KDa

Sequence

String:

MLRLSVGFLM RHIGQDVPKR HTHFVLESRL MYEKSFRDSW LHSVCRAVSQ IDEPLSKTIS GTRQKMLQRK VTCFQYNQYG LFKVPYYRL ANVDRYHAVQ GVPGTREWVP YANVSYWTMN KMVRSGNLLV HRVHYTGWGT DPHLKKGGWE HRWNKVMQRN A LQYSRI

Macromolecule #6: uS10m

• Macromolecule: Name: uS10m / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 94.283742 KDa

Sequence

String:

MRRGIVNHYY WSRYRMPTQM PKFDGPAPIA APQNMNSTKT NEFIDPIDDK FPLSIRGPLV RPDVPEDQYV DSWYVCTSMT HHLGDYRPW SASAPPNAYR FRPYNEFDAK GREYVEYMRQ FARYDPRKSQ GKGQKGFPFR DAYLTKMNEA NRTTPPPTLE T IMDRAVRE KHQHARVLSP MQVQRDVGRS ETPLPCAGNI PVDRSQFPFC WKTEDWYEYE VAKVRNKRFV FENTEEDGIH GS EVTYKIV LEGFWDHHVM KLAEDVCMFL RDVGRQVTEE KLVAVRRVME GLTGGAFDPE LIDFFNAARA GPFGRPDEYD AEE VANFVR ADLKRLEEQC LNVINRCNVP VPGATNIYDP QVSWPYVEKL EPWVRMAEFW TSSSDTSFTE LEMSTAHYEF RKYF RVIIC KLPFQSTEFE KRMYDIRHWL HRQTTCEFHT IYRKNVIHDG SVFPTEHDPA TPTTHDHHRM FSFALDWQSA PVNRL SVAR VLEGDDWERV AQRLGCSVDD LKEANPAIEE LEAGAVLNVP GGASRRLTSF GAAPRVLPLQ NPNNGKRIRT WEDAAT VLD CTVEELQQAN GHAALTYKQT GENGEGEFGP SVTELNVPLS CWVSTAETEF SPVELVFAGD TFATIAQRLR CSEEALK KA NDGQSDLSHA RFVRVPAEAK APRRLLEPQL RSQAATDVLM TRTIAEEAAY GIKNIPDLPS NASKFPHEYH TPTSRFPT T PKEKESESDW MAYTARYLDK QFTHPTEPTP IYNVNKLWPM QQVPGKIDQT PFEEDQTWLL NPIPVQQLEQ HHPEKDLQD LPFVNHEQFP RSLEWTAP

Macromolecule #7: mS72

• Macromolecule: Name: mS72 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 19.403363 KDa

Sequence

String:

MFGTTRVWRN TFLTKSVATP PISVIRTGPK WWADPERMVR QKLMYFTLGV DQLPLRRTAV IQKDLHRFHM CKPPPRIGDT TGYKRSRAA QLTTWYRRIQ YQEYHLQHLF TRHVWGLVRA YPGNTTKIQG KADDGYVGYD SVPYHRYNRT PLPFPAREIY G RRE

Macromolecule #8: mS71

• Macromolecule: Name: mS71 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 25.866699 KDa

Sequence

String:

MYCYYLHTSP ASNENDVCHS ASAKSNFIFP SFSSLVGFIV FFLWKRQMPL FHRLFVSGAD LRGCHTALSS TFTQRRYWAK PKKRPKVGQ GFHEKAQKWR DEFLLDRHRI LADSLRAYVE FSASKRTEPW DTRFRPFDRV EKDGVYVLMR HLMEDKFQLC N YHHRPVKR LFCNVGLLGP QVTTKARWKP YRYATNPANT SKAERIFQKD KTLYTHGHND

Macromolecule #9: mS70

• Macromolecule: Name: mS70 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 20.679938 KDa

Sequence

String:

MRRTIAALTA TPERFSILGT THPKPKRTGF GRNNKMRSKP SDNVAWYDKG PVEWLPRPVR LTYDHLDQLR DWMMRETLDG KTEEFNRIR DMHREWSQHP LMPVLGDVEP KFPLNLFKQN HRAKRRFLVR WHKANTPANW LWMPRGPTVV TPLHHTNPSQ Y PESWRQMV RKKK

Macromolecule #10: mS69

• Macromolecule: Name: mS69 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 21.672887 KDa

Sequence

String:

MQRAGCGIVR PGRGCHTTPL YCSLATISTG VFDHLPFQHR RQHAFNTLPL HDANHFGGRT AYLREIGPVN IKKSGRRFKK DLRTVQFNV DIWCAQQTLR KRWKQRDWEV IEVPFRLAPA EQQRVIPEMY TDVPPMTDPE RHDFSNIRNK VYDREELQGV L FGASGPLP YPPLQRIDRQ AMTLDKFL

Macromolecule #11: Rhodanese domain-containing protein

• Macromolecule: Name: Rhodanese domain-containing protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 30.180223 KDa

Sequence

String:

MLVSCIRRQA DRRTRHHMML DGQINPREWM NWHPKEYQPW YFDRHKFLYE RQQYAHFHHL LCHQVFLKDM LHLVQHPEPY THIVDCRTS PLKMHRWVPH SWWLPRDEVE YALQLTPDEF ADMYGFHKPR KSDDVILVSH NGLYSEQAGW EWKKQFYQHV Y NYRGGTNE LFGEQYNDVA LKDSLPPWKG PFPQSGIFVD KWSKRKVLTR TGPFDRQYEM QDFALPDLEL ERPRHPEEGP RQ NMPYGLQ

Macromolecule #12: mS58

• Macromolecule: Name: mS58 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 35.017238 KDa

Sequence

String:

MSFRYTNGLV GALKHRMMLE SSHRELVRRR FTGHCRGVEV VCSGYGTVLA VRLVDKTVWE PFYRKGHPSP SGADMDSAPP SSHAEGTPV GGQSAAPLDF ERIAESIKAA LWDATRKIRS AKEAALNRSL SHNQQLRAQA HLEHWYDEDA NTLQPLAFEA L KHEAATPW MQFVQFGKYK HAAAVMHSES GGKTSEAFTG ETKRAGADNE EGPCVTALDE KDVDPTSIPI GSVHPLFLPA LI QFESRVD NSLNDDAIRQ EQRREMSRDE QLFWERVELI RKGQVATIKG GHKRDYADEA AVASDNAVDK VQLRFTQ

Macromolecule #13: mS57

• Macromolecule: Name: mS57 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 46.272082 KDa

Sequence

String:

MLRRTSWRAV GYTPVNPDTS PMLAYSQYHW HYNLPQGMER PHGVNRTMTA PYQSAHSLVN KYRGVWIELD MHPAFRVALE PQLRKLPQG RTIPKTSVDE VISDYINTAH LIQDEMTRDL WLAKVLQHCA FQRSNEGMAL WEKYCHSRFI ADGATATPPL P LVKAILFY CSKIDYQGWS SIFQKCLKND WNYTPLFDTA QWNFLLKSVG RMGDEKGVRL ILEEMLDVQA DLDRVEARSI VI ALNAVTD NDIYEYIKKY LFNFGERKVK FLRIIYSDLR GHGAGKLRIP LKENDKMFYH VCWHSSIRAP RQFSPRQLYF DYT PSTLGA SVHNPNAKID DIVKDKIEKW KTEGLLPEDY VHEDRVYDRG TAFKNVARQE KWKKMPRIVK SKKMGYTGDP

Macromolecule #14: mS55

• Macromolecule: Name: mS55 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 67.008211 KDa

Sequence

String:

MLSQNVAKTT VPSYYMIRTN LPQRKPQNQW EGVYYFGGIT KRQRHLILLQ RKREREARMR AFSASCSNLL RLLEGDTQEQ QQAKTQTIQ LSSPHGPFDL AIRLAQHGLY QQASRIVDEL HQQRALRMSH YGLLIDALSA PCLGQRILYG SAQCDPALTY K LLGDENGE ERAQEAHRWF DMAFALLTTE CRMSGSEHRL PQATAAATHL VNALMRALLT CGYTHVSAVP DAVYDRMGLM GI SPTISTY ELVMLALSLQ GNMKEAESVF SFLRRHHNEH VTIGSFNALL LGHRECRQFD RCDAIWQELV DRRWPRASTL TAE LYLRSI VDHSYTPTSG PLQRFGNINV VEKKKIPLVL AQMDDLGIPR AHLSRPLMDE VEDALRKFHI YKSRYYEWGR AVKQ FNFIE FRRRNGWMYD LHLMKNTTKQ VGPLRDFNQP DATQAPVATV EIPAFFNERP AWEQPPLEET LYVTESRERY DDVRS GDIY EDRTRSLHDR SPTWMNEVPE TRYDHLYGVN HPDIAKIGIR RHLNAEYVNR KEVVERDAAL MKKNLSTGRR LRRKVE SSR THRNAGSMSG AAPASASR

Macromolecule #15: mS54

• Macromolecule: Name: mS54 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 77.912539 KDa

Sequence

String:

MLRRAPCLLA TNIPRSAWDP AHFNTNWSDS YSTEIAARRH WPAKKWSIGL EPRTPRDWLQ FSYRNLAYAY NGALRACQSF PEMLVCYKE MKQRGVKVDV DTMNVLLTRA ARYERIQVDD VFLLFDELTA LGARPDIAAV ETLHTVLDHS AAMPYEWREA R RRQLVELY NCLAMEEIER LAPHRVDRLL KEQIKRYRDN LRALKASLSP SVYRRYLHTM HSASMLLEEV HNFLWELVES DH PAMEIPA LQLRIPFVGS VMRRPETDTN EKLVKYTDFE DTDVCSVFLA AAERAVDADL HDTRAVSERR IFLSLLTMIS YSG VLYTSD LMAQLMEMVK YSTHASSRDS DAQRLLRYAV RGSSAAQDDL YRSLWLKVEM VADSRVLGRY IGAREPWSPI RVCF DERGL FKSYPPLVHA SAKKTSAIEA DATSGSGSTT MEEEQRQQSE RQRMSTEITS HTSKGGEEMV EGVRGAVVAE TSEAS GVKD VALQEASHGE QRTVEALDLR WGDIHRLIER TRALTSPPTE RHPQQEKMEI FTGIAVYLRT IATGRRYCSG EEEAGG ESP AESRERALFA VGYELDVWAR LFELVQEVRH DMEKFITDNA AHHVEPEFEC WEALLITLRC ILDFCVVRTQ EKGKEER AA VEELFEKTMK LRNELVEESR TRFGGRMRIL WLQEA

Macromolecule #16: mS53

• Macromolecule: Name: mS53 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 76.246633 KDa

Sequence

String:

MSVTGVFSKG RGIGHAAVTS ILRYIPRARV PWQPSRFGRE NLSASDLAVL WSRGRYRDGP GNYNSGYHTE KTHVLEDNTV TMIPKHELE KYMPDISIGP KALVTPVSLM SARNGHRVTH DLLHSYDPHI GRLDKPAVVD HDNITVEDPN RVGLNAATLD C RGRIYRWL RRGPFFQEDH YFRRSLRLNR DGTVPTAAHE APLMRKIVRL AQRGHLKAAC EEYRRVTTVP PVEVYRALTA CC IPGGLIA DAVAIFEDGN SKLFYVARDG EVLHNVMRCA IKAKNRVRVM WVYNVMRGRY YENVIVRAEI DPIWRYRIAL LAL EYFLDH NCAEEAGTVY SYLVEEDLLQ CDVHLRVGLH MREALSKGKS VGLSDEVLRA TSLVTDVATV APEVARELYQ RHVE ALREN EKSNDCDMNT KNDGATGCAW SAHGLLTALD FTQKDDALPW MQQNFGDVDV ASVLRWARFY HSKDLMAKDR PRYLA RAVA WIELLSKRSH MMEEAPLTYM RKSKPLSLNT NSNLRVAWQT PVARPDGPPR LLAREEGYTF HHNEHSRFVT ETYRHP GET LQSRFLAMQP IHTEVSAKED FQEIYAQQQE QRALPSGVVS PTARILHHSV TSEIHGGGGG QHHRSMSRAE SSTGGAV KK EKLNLVSPHV AAEKRGGSGT GNIGGTAAGG GVTPEF

Macromolecule #17: mS52

• Macromolecule: Name: mS52 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 81.423836 KDa

Sequence

String:

MRRRTVISFG YVRVAASAVG VIGMGANHRH HYQQQQRPIT TRGQFNPVHD FTYAMERGVR ARDEKTFEKL ITNPGPLRIA YSPDYLDWL YRCYKAKGKY MDARAAAEKK FNGNIISGGA VTTSSGEMIH PLPGAPPPGM FLRPPNSFRR LSGEMKRKHA Q ETLDEVSK AQGMLDLFER QPQFPAIHID RCTRFHLVEL FKEMVLERSL EAVAIWDKAL LYRAILSERK ASYPASFRYI FK AVEDTVF AHSSVNCPSL EAYYYFLYLV KKYYIDNAVE AHVVLRCHRE PNATDLLFSN PPPKDEVDVR NAIEALQSAE ATS HAASSA TSGTAKHDDP AATDEKHTRG DNDQKDSANA NAKHPCQFAP PSSYPPIEAL WRCEENVPLL EILLFGEFNL IVSE NPFVK FPTAHAFLTR PYSTESSKGP IDGASLANVI AEKRGHLLPS FPMNVASAID GRAQELRRLQ QKHHRDDTVS FQTLL RSTH VDDNPSTFSS YSDWSYFNPR AVRAEERDRL TRKGIDALKE YDSATEDIYR RSFEDAQASN FQRVTEAWNT FPPYLP TLP HFVSIIKKDS HISFLLHVGL PERCSSAEAA AKHKEFERRI YQLARALYHT ALEFHKETVR RVNRQKVNVA ASLLDNF FE QEWVAMLRES ESLENSLEQG AWPDKKTDMA RRLGRYIPFA RRSLDENGFP TDARADDYAR WMEAPARMKG AA

Macromolecule #18: mS50

• Macromolecule: Name: mS50 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 129.011102 KDa

Sequence

String:

MMRARRVVVA LSPLAQLCVH VQWRLYTPIW QPDPAVDHVA PLRESDENRT LWASSAPIAN VSDAIAAWIR FGNDPVLHTA LPVIHAGQN ERTRTDGSSA SLSLSSLPSP SSTSPFATVE DYMGTNMVFG SPEHVKDSAA VWASYFERRY LSQLRHSRRT A ANHVGLVN APDVFTDEAD RPETKWSQDT RFRERAYMAE KFLKEKVANL QQLEQALKQA KPAEYIAFHD ALQQQTLTLI PL PSPSVWH YGGARRTQWA ERFLPLSHEA QQFFTTVLAE DLKRAGDAPE KVLQKVAAVF AEVGKILLQR HRRCLGGREW SAL APHEKD EFCMKEVERW KQQVEVGEFD PPLDGDDDPT STEWQSEHDA IMQLMTATID GLSFSALEFW THTIRCEEME TEHI HTEKR VRAISAAARR AMYDTTSYEA VLQGIVDAVA KGQLDMKAAG FKPHMNDIWC QLNYAKFGAS TVTQHTTTAR RQLNY FHAG LLKEVAATAA LYYATKPLSS SLDYASPYKF RRSLVGLFST YGVEMVYAVQ RPLLFSAANL AKAEDLIRGV VKNVAR PFG ERRRAKLKQL RANHRRLATP VQGVVVSAVV SDLLESGADV SEAKKAEKMQ ESVTFWPLGA RRVVSYDWPT PHFDALK RR VAAAGSAVTA QSTKEIQEIK RNAFVEVSLW RRVTAEETKQ RRDAVEEETR RVADVVRTIP PLAQVQQYAT SLYQRIED A APFPAATDNN AKSEQEDDES SWEFVVMLDD RVVLNANQAA ELYLPYTDAS GVPIPQGECR VRVRGFDVDV NPTLNPAFC SEAFSTPFQV FDAIPQLVQQ FFGTAKPSVA EVSDIPSSKF IQFCAFLREA GLDVPVQCEF EAGQVLNAEG DVFMEYFLNL LRSDRFHRS CAQAGLTEMQ RVIESSCRAH WEVHHPGANE AEWAEARRRV LDRAMEKERE WWFPNEMLDV TNMSPGSNHG L RLPMYPAT VRYGRELCTL LAAEGQFDNN SGLSATCAVN GTGAAESITF STGDHISSTF SMEEALAVAK GALRNAHDRQ NT LAAFRLG PLSKHSQVLL FCGINATEFG GKYARTYTYA FEKAKKELAE TFVSGRVVPG VDEDELLRVS DKEGVDRFAS STH PEQRKT QFVPRVGPGG APIEDPTADQ KTQWGR

Macromolecule #19: mS49

• Macromolecule: Name: mS49 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 136.204141 KDa

Sequence

String:

MIRRRLCFVS RPTKAASISV TLFSVQRQKG GLHTFIRDAR SSSFTTPRQA SHAEGEHTSS SLNSTDWATQ MQRELFGETD PLGGQAHKD YYRDPARGYS PQYAPRNFAE GGAISYHHAQ SPMEYAEATH RRSWLDHDVA RMEAAFQEQR ALLRGMESAT E RDELARRY AAEHHVADIV VENQSLLPST QVHHSTSTSG SALRQQAVVD RFQIADQQSP LATSDGMGRE ELAHTYRMRS ET VHNDWIE ENLRIVHGLR EKEKYDFTVL QRATRIPFQG YDMDRFLAQQ KGTPYGAQSL PPNTASSTME EAQRTLRDPT ATV PSFEAI SQKAFARNTV RDHPTTGEEL TQEVVDTIRT SREASEWQRE QERAQRFGLG RQGALVQDGG PDKRTLKKHV NDER IMDAM FFRSDAYRKT QTDEHWNPYM RQDTTHGVAH LLNNKFDIAR REDRLSKGEQ DLTERSVMHF GVPIQQTIDE FVFRH RNAR GERPLDYFKP FPGFRDFRLN RMYRDVEGFS LMKQRPEFLE WELFTRYRAH HQQRRRIALL HGLEPVANET AQERDA RRE KLDEICERTP FDERELHTND DEMQVSGETL RSWFGVYMLP SPTVVEAVVG ASASVNLHLF PLADEMGTAD TRENVLS SR YFNRLLLMEG FQNRISRAFM GNVSGKAPEP VVQYMQPPEV LRHFTAEERA MYEQYVKEQT SKQLGEWATA MRRRRWIP D RQQYGHVVAQ GYGVSVVDLE HADTAAVLTV SAKAFERELA AAKGNTSHII MVEGQAYKLR PDSERFVVPL SVRLESGEV LDMTDEAFGR YELELLPRNV NHALNYGIGD YAYNRGNYIE TQDVIWEEQT ASGEEGWSPA THADGLRAGL PVRARRHVGM NANGSRIVS SPQRAVIVAY DRQPFFNPEP RLVRVAFQSD GSVEEVPLAN IMIWQRRYHG PERTVGDESR RFSPASLRRY I DVSDPFNE KKSKGEHFLD KYEAARTSEV AAGKYRTTKQ ITEIDQWTRF DVSRADNFRP LSISHRRDYI RLGYMHRYTP WE WIAVQEA DQPLIAEQIR QDNIGTSYFF SLNRYWRYKA RPHGYIRHFD NEVRDLFQFV DGVTPWKQAQ KIRTYWEVRA HHP MPQFNR PEVAMHRNTV GLLPAHMWET DKKTGKVKAV KDSVRDYQTK TPLPKWVQL

Macromolecule #20: mS51

• Macromolecule: Name: mS51 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 95.139117 KDa

Sequence

String:

MFLRTHVERH RSGKARAFVF RDPTLKMMRA GSGYQQLRRM GMPIQVSKGW RKVDHFHANN QYQHAWPLLS HDDLGNSDQS NNTRNIMYS MYLPKRNKGT APWFRGADTY SVKYCEQGRY EYQRYLMINR FPSEYRKHFM NFLSNIRSSS GPATIPQEAL H WLLRMIVD NFNPQHVHYI AAMKTLQNAG ELDMARDVWK IMERQQTWPC TSTICAYLDV CVEAGEKTWA MEAWNRYCTE LK FLQPGEV DPKPVSRVPF SLTREELLYL PKWKKHFDHD PNLDVVDLNR FNRTREVYLR MAQVMLAGGE RDSFQHFYTK LEE AMLSTP TPVPEPPNPH LVRRPQWSPY EHCKSVHHSP WRVGNNGRAM ALGPSLTTED EMQSRFFSND QFLVHMLKEI LRIV LQEHR RRHPEACSRG EGEAFFDQVV DARETLNFCN ELIERLFAVL GQKMHGLNTS SLLSVILELY RVMGKETGMA LLRRA NQFL ERKAALEDGA KESLTAPNYL QVLMGFADES AYVYDSKRKG LCRYRSGFDP RTTMQQLAAT VQEIAGNPHV TWAADM HLQ VVCTMVGCGT MKANDYFVRN VLRQFCWDSR FLEALYMEYR RHDDVDMWAE LTKRALVWTA RYNVNASERL KRLIEDD YD TIQVHTRTFR ELAVFQFRDV EEKRHSRDVV NELPNPWTDY VSHALPFPDR DAGYPDEYGD IGQWRAPGGP GSPVKGPG Y YAPPMEGEHQ RGYTAEWRDL KNPMRPPEFP TPWERKYKQY ARGQHPSYDM VYAGPMPEIF PNRYDFRKPT RWDFHDIEK QGKYKTSGPY

Macromolecule #21: bS18m

• Macromolecule: Name: bS18m / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 37.204203 KDa

Sequence

String:

MNRMGGSVYA NAMAQFAICR QPWNEYINLL TKQDSTPYHV EPQEKPAYRG RKRGREGWLF GQQVQLHYHR FPDEQLLTNL TRWRTGETV GDIALQQFRN AQPFDIEDKD PQGMQRPSPE VYMKLNYKNP ATISRFLTRT GHMYPADILP LNPEAVAKLR V AKAQAVRI GLYPRFGNPF WFRSQKFRPK AYQENYDPTT YSTKHTMEHF AYNWVQTDRI RRYFKELEEL QKNASNGARG GS ATTAEQK QQNQFYAPEN QPISMHRNNI SYMAEVERSM KNPTVPGLMS TKGMKKKFHN LYSSTSTKRM GFSNPTLGIK KV

Macromolecule #22: uS15m

• Macromolecule: Name: uS15m / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 50.080918 KDa

Sequence

String:

MFGRTAFLLR KYRQKSKAPD LKTSFSKTRN FKKAEKIAIE TVDGEVVKGR GKLRRRSHSA LKKAIGEMEH PSIWLWYPWR MNPEPPTPG MPQRRALKNL HGAVFTDLTP VQKKRQEQML YGINIPETRQ MKFEQEHPLL ASALRQLDGQ PKGFPFWYKK Y PTRRHAYG NRFSIPDEML EGYGEEMKKA LSKEMMSIQE KQFAQEAMYM ERYAEHDFDT TSPAVLAVKR ALKCRVLRNH LL TNPHNNI IKAVLANTEK KLSHALRKLR KVDFKKYWEI IRDHDVQDVL QPSNLVTYRQ GAYWKYDWNA GLAISTNLAD VLD PRGLNG CVETGRSRSE VARDLGLSYT RPLQENEKKQ LSHQALYYER LAKFKMEQPE AARALERERF VRKFSGMFAK MDIK SGAPD FPSTYRKLLG TKVVRWASKR HGPA

Macromolecule #23: uS11m

• Macromolecule: Name: uS11m / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 37.723637 KDa

Sequence

String:

MRQSLVLLRR GKPRPRAGMF PDKYRRVPAL LKPQQGGQQF FNQFLIRFTN DRLMRRDVED GEDKKESKIA AQLPQMDWEH MSARSSSDA IREEMHRLVE GDAVQHQRVF NERIWYEEEE RRRLQTGADA AAPTEDAGGA KEHDIPPRVL GNDYFQSRFG Y SLVKQSEM PQGVTDYNQL DMWGEMPKYT RDMVFLYLIS RRRNTYAVAY TYEGKRILST YTAGNRGLKG GDRGFRSDGS TD NGHQVTS MYLNDLLPKV RELRANEGRP IGRGEKIELV VRVMGFYNGR QGAVRAVQDR ANEFHVRYFE DITPFPLNGP KMP RGVFK

Macromolecule #24: uS8m

• Macromolecule: Name: uS8m / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 33.219047 KDa

Sequence

String:

MLRRTIVPLA KVYGAAPPNG TGQMQPARHR LLHGKRERQG SLFAIANDVK YDEKRLRQQL NAMLEEERLP PRTRLERNKA NGGEALPQR RLVDLPGVER RRDLPADPIT RLFFQHKGDH ALYYGTYDNP SLQDEDRIQI EKREPRYWTY NVFTPVYDFC H RIREATEQ RKRFVIVPST VETRGCARVM LGHGLVAGFR DFHNDRAFAV ELKYFQGDST INVIEPCAYD GKTEFEWSPK MM RRLMNTH GIHNRLVVYI CRTADNRVID HIQAVKENVG GRGLIMVH

Macromolecule #25: uS9m

• Macromolecule: Name: uS9m / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 50.33525 KDa

Sequence

String:

MYQLLSPRTA RHARLFRLAN NLASSPSGTA GVPKTDGERL LWVNSHVKRN KDIEMSIEEE SLRERQLPLK LGENAFTSSA QATHGSLFH FREYPMYPGE YVPAGHNTLS SLRHELRLEL TAQSLKEAWM RISGGIYFQS ADDYYASVDG LDAEQLGEVL A ALFPYLST YEAQALVQCT LDSISKPMNT ASRQLSRTIT AEAVGLDNAP GHYTNFLDWM GRLTETRGFK TEHALFQFSR RK FNRDDVR VMFENYKLMS RATLIADSAD SYSHFYTVLK DFARKVAGED SRHQIGVRID EPEVDAETGI AVGRGCADGE KYQ FTALLR ENRDHNGAIT IMGKPMALVL DNKAWLMEML LMPFDEANLD YRDFDVHIVL EGHAMPSIAN EIAAFALRMS IANA LVKLL PLTRIPLKKS GLLSVDRRRE RGQFPGYLDG KKVKRKFAKR

Macromolecule #26: mS73

• Macromolecule: Name: mS73 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 24.784449 KDa

Sequence

String:

MITELRTKGA TRTPAIRYSY PAPPPSKNAA PQQPRGTPRP RTAKTNVRKP KRSDARRRAR AGSGFGVVSP VRARRRRVVG PPFADFFGA LFPATRGIAF LSDLPTHIFS SFLLSFLAGR GVAMLRMCRR LAMKYADLEL TTRGEFPHGM KEPGFVKKLD Q NIPWYFST YRSMYHWPIT GDNWSDLNEA EKHHDLHMFY TLAWWKLGEG IFDANDEDN

Macromolecule #27: mS68

• Macromolecule: Name: mS68 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 27.692355 KDa

Sequence

String:

MFRISPSLAW RRTAAFYLRA GKLGQYEREA FEARRRLEES KNYPGPIRSA TPGDTRFYAG SLESILQDND RHYWRAVIDD PQVQYVIPL RIRFKLFTWV TTGWEQRLHI VQTMAPRDIT IARLIELVTI ENQSPYLCSS TFTLAVDGKE LDPDKSLSDY G ITEHSRID AIEKLDHLLH KDSERPLDWT VDEMTTECLK RSPYKEMGMQ PQPNLAPRYE ARPKGYFGRN NYSGMKQES

Macromolecule #28: mS66

• Macromolecule: Name: mS66 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 29.834234 KDa

Sequence

String:

MMDRTRLFLA AEFKQKSRWS SVWPNMHYGA MYLSYSIGRK LPMKGVNWVT RESNRLTNFS NRYQAVINDI DVKKTEEELG ITLQDIRWN DHRRIYWKCS FCGSSYRKSV SVRTKFHAGC NFCKGRYPSE VLREQHQSLS LAASAPELIK QLKETDKKDN L GSLALTSK FRAEWKCQSC GGSYRASVRS RTGMVENGQC PLHPNIVDWS AYCPSCSWRP NMEAIAEEVQ RTGQFLGLEA ES RKIASAP PARIPRRKKL VS

Macromolecule #29: AKAP7_NLS domain-containing protein

• Macromolecule: Name: AKAP7_NLS domain-containing protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 30.783395 KDa

Sequence

String:

MLRRTFAVVQ KGERGLYFPI NHRIVDRRIA SGVTVEEADV QSRYRRELRT SFATGETRQT IPPAWSACER PTHFLSLRLP VRNVLRTRV NEMHNQILFS HQQHAPLLVP LEKLHITLGV MAISEREETE RLASIYDCVS EVFSVIHPLQ LRFRGLGTFG F GRVLFIRV VPEADFGILE TAVSKIRRRV GGELKVDMKG NPHDSYVPHV TIAKIRSKQQ TQFGSKIPIS MWVEYQHHDF GD VTFSQVD ICSMRGSKDG YYHTEGSVHL L

Macromolecule #30: mS63

• Macromolecule: Name: mS63 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 34.337473 KDa

Sequence

String:

MLHGSLPSLA SLRYRRPYWM LFLKDVDNWK IYTVIQQPDH QRTEMLYQAW LGGLDRPYTR PKCMANQPLW LSKKRHILRK DRLDGPETP LEKYVLEWHK RFHSFQGTER PTVDDLHTAL DLVERPLDLS YAFQLLNQCR NVNNIRFAKD TFLVFLEACL R VDRKDCAL YATENAEALG FWHIEEDYRR YLRGEQSWYR LSPLDNMYYP LEENVKLNAG RSPPSSAAVA EGDAEGTAET TG FEAAAGA AAWSDDEGSM KAGSSRESMT VDDEIARLEA ELAALEEEGT DGDNDVKGPK GH

Macromolecule #31: mS62

• Macromolecule: Name: mS62 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 31.623609 KDa

Sequence

String:

MRRFCFPVAT FAQAALRHRG IRWNTTMADN ESHTGAKSSA SSSTPSEANE ISAMERASEA RREIHDLWMS TEKMLDLENR VRSVASLIE KYKLDPSTPR ENDVSRGLGD AFDRLLLLCV PLGKDSSKGT DDLERLMNLA GRNGREISVR TIQHLFARTD S FSEALAVF YAMRRCHVAM NMEAYYAMLY SLQRLEEEGW AQRFREECEE KGGVSEQAMD FVVKGINNAL LPENKPWLGR VM FGDRDAP AQRREARDYD ELSAMWTERY RDGSAFPTSP

Macromolecule #32: mS61

• Macromolecule: Name: mS61 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 32.920785 KDa

Sequence

String:

MLRSTRPWRF RMKGGEMFVE YKIMSRDHRR SIRVEDAIVD PSVARTVVPL SWLEQLRSPS LRLHTGYHME EAVYVPPAYA AVDEKEGRR LSEKSTMTPN AILAGPVVLS ITGQSVPVVL NPYFVPDDTW GIRRNRDEWD LRLGMDAIEQ CTLFSELRPG G LLYNKLPS SQNVTRHEPV RATLQRYGMK CGLAESPLVP RPWTRMRYMF IDELQRGPKL TEFVGHNPRN GTQWRFSQHS KY FRIGVWR ETIRRNDMNE GLHGHSSWQK SPQQAVPEVR LMAPYP

Macromolecule #33: mS56

• Macromolecule: Name: mS56 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 47.860852 KDa

Sequence

String:

MRSIRGVCCF SSLYTTQFRV HATYDVAPLS HKELFSIYQN WDKTRDELDL LEEVEERISK WKLNKWEMRI PPLLTAREKE LMRQQQELL KSIFFDWGKC RDALNKDLEL ISSITGLPKG TVREKNRAWL QEEAAKLRWV GEVSKATRLR DAFLRLEVYG S RDHRLLER LCCIYGLGLQ GSFESAFSNY IVEDPITKKI YVDEKNSFRD LLAYIIHTYP QIDIIYDFLG FNFIGGYRSS LR RYLECMV SRSTEGEKIP GRLVFGRGKP AEILFDFGNS NESLVSGECT QGFPDFVFVK GSDMTLIIIA SENSWLRNRQ LPH RKQMEG IARRASFVLG IPFSEVRVRN LLLPPTYLDK DSIVRINEAV LGLSKEEQRN LAPWLEMYQK ELDSKDVDFC SLMK STNEE EWLTL

Macromolecule #34: mS60

• Macromolecule: Name: mS60 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 34.410688 KDa

Sequence

String:

MEVYRRITER VRGALWAHHG RLMSERQFHR ILAYVYNNKY EHQIRFDRME VVGRAWEGRV EVVTTPAGYL KRVRVNPCLE ELSSYRQQQ LILAAYADAC AQGRRLMEKA EINIYKQFLK DLKPIVMGIR DNPEFYTVPE DSVETVGGTL HMGQGPTPTT Y RTIPAAKA HIPVDEIRAR QEWEKKWLNS PQGQSWALTL RGKRYFALHG PQYRPRGAPG AKKVTMPLDL PAPYTSMDER RL LKKNWMA YLDNKHVAEV MWTRAKIADR EKLQRRLQET GQAWHRPINK EAVSRW

Macromolecule #35: mS47

• Macromolecule: Name: mS47 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 134.734562 KDa

Sequence

String:

MLRRRVLPES HRHASWPLIY ETSQHGIKTL DVREYRPLAT PIEFRFYQRY ANHPNRQSGI QFLTHYNTHQ RFRVNKDYID YMHWGKEQG QARLPHRHQR VAFDFDDQLH PTRTLDNEGD SYAWCAEQDP TLGPHPDLDA SFDPNRRVFS HPEHWNKMFS K RRPGEGRI DLRVLPSQSL LGPLMEQSDT QGAAYFRYDN RGHSNGRVPG LNTPFFGEFD RKMMQAMSRP LNADRTITGN DG RFSKTIM INEPKTHQAL SGKTASELSV EIDKATNAVH SKLTVLEAAQ SGLTNYYCGG LNFEMLGFDL HMAEMLREKA RAI LNGVAS VSSTSVMVTT TSVPTKAQER EVGQLLRDAL RYEDRVDDAI RQHASLIWRV YTAPRPLMAL TNGKCRGTGC GVSL YSKYC ALKDASEFIF DGPNLGITPY GGLTRLLARP ETSLKYPGLA EFIMLTGTSL FAGDALRLGW TDLFTTLPDM SYHIK DWFD TTEHMHNDAV AWQLGHLLET CFKMKEAHSS AMERVAITPV RARWIEDSFA DQPSVNHIIN TLSEIERLPI TAKQNT CDQ TRCTPYTLTS VEAGISKLEN HRLRYTHSPW DITPPEDEVS LQHASEIFNA YVLERRGTFN VVVHRDTEKL AAWNRQR QE EYHAYRSLRA APHPRHVYAR LEGCEGKLVS FDFVFSLQGK QKDQRSGNKE GEELQQTACL DALKRQVLTS FGMPDGRD I ELGWYLPTLD TCPIHNDVEI MQLLHADPGI EDPKAQLKYP PIYFIVKRNC LYFSEWAYAV KHQLLLQSPF ALRAAYEML LEVRGDGSAE RVMPLAESLA TEFKYISRLL RRPDFYRVGV HTDKSAEAWE EIREERQRNL HKTHQPTRPL PDFEDVFERN VEIDGHRFL LRPRWSPRTL QEVLDADVMR LHTSLAYQDE GIAPLHVPTQ CAKANRISDM VEDAGGLEVV PGLGELDAKG T PVVPPLQS NAHVPQNVSF YEMARHPWED AASSWRRDGF TEGSLANYEA QYRAAERAVY DEEGRGGHNY WPSREASEGV TS EEKDAAL LRERLFKPLE EALSGVEPWA RNLRRSASDG KLGYKTEIAT PEEKIYDDEY YRWFIQPGHH PNPTGLTNVH KGS STLQGS DAAEFERIWR HVVSGVDGGN ASDEAETAAE DGEEEGDTQM NEKNPVVPND EEEGV

Macromolecule #36: Sod_Fe_C domain-containing protein

• Macromolecule: Name: Sod_Fe_C domain-containing protein / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 49.030516 KDa

Sequence

String:

MLGITSALLC VTAGRAALVA SELQKLVPVL LPLLDPPIAK GVNVSAAQLI VASGHTSRRN DVHLPDCPVM AHALPLTALA EHSAFLRHP CRACLKRRVH FLSSEHFCMH LEEKCSQGGG GMPAEWESIA AGQFGLFTHF TDEFVMYATS RREPGWTWLV Y DKERPEGH RLVVVNFPAN RVPLVLGLWP LAVINMTESR LLSAIGARTK TSAAESSPAP WSRAARNPLV AEASTATVAA MA AAVTDTV DGLDGSLSSI RAQVAQEAVL KMNWGFVLQQ WKQAESYYES AERERKVQAQ RTKMEREAAL AEMSKLRDSG ATI LTSDTV EITVAGSDAV PEKASRDLKA STQTTKEPRK EEEETAGVGT ANQDPHCTQL EDGTWQYAYK NGDLTLLRPD GTRV FKKSD LTTTVFADGS TLYEYPNKTS ILDRVDGVRV TTFADGTTKE GRYK

Macromolecule #37: Superoxide dismutase, putative

• Macromolecule: Name: Superoxide dismutase, putative / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO / EC number: superoxide dismutase
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 38.998422 KDa

Sequence

String:

MAVEGKKFDG REAKKGLFFS PHAWGWITHG MFLLLLLPMP LFFCSLWTHL RECCCCPSGP LRLSSGHVER HAKGIVQQMR RTLRHGYHA YVPKGALLKT DMLASSPQLV TAVFRETEAA AERADRANDY AGFFSRPRLN YPVASGIPAF ISRRQFDVQY N IFHHDAVA TLNRHTLGTS LEGHSLETVI RRASFDATQA AVHTAAAEHF NYCFFYKSLR PWGTAVPKQL REAFQLQYGR DG SVDVVEE VKRLFTVVVL SHQERCGWVY LVWTGKQFDV VEFPHGACPI GSDLIPLLAI NVHEGAYSLD YGPSGLEQYA QNY FRACNW FLAERYYLQA TGRGSSCDA

Macromolecule #38: Protein FYV4, mitochondrial

• Macromolecule: Name: Protein FYV4, mitochondrial / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 22.392725 KDa

Sequence

String:

MRSTCRFFDQ TCGPHKSYKY TYMPDPRKLA PIETTTRSEI LPLVIRPPTS YVPNHETFLE KVDIHRLKPT SDFKAAFKDW NDLMSCDKR QLRVRGIPRM TRIAIRNAVH AFQNGNPPEY FDTKEEWLYY KQFKTIDFSY RVIPELPEKY RPHQNGIDQA P LPDYREIN KMPEWARKEE ERLKEKKI

Macromolecule #39: mS37

• Macromolecule: Name: mS37 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 24.565621 KDa

Sequence

String:

MKSSDIFHAY RYTPVFLKAR QHDSGVNQYG LKPVNAYDFI NPTNLVNFGR GTSFDNLGVR RAGRGEIDSS PSLGGSPVFT QAKLVGLSG EEQLTMCQSE TMALRVCMAR GGQDTCERES RALDACLSRV GHLRRAMSEA CGEFNDWFIQ NVSDNHTKPF Q HRPHDWRH FYAQEKLVRE RQQNGHAYGR RPKQFSFGAR YVKTEGYGKR PRLPYNK

Macromolecule #40: mS38

• Macromolecule: Name: mS38 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 26.499914 KDa

Sequence

String:

MRGCGVLCAG HKRAAVIATT TCSLGPPTLS PSLTLRPVAC AATPVTIPIF PPPMRGSFID RNPVWASFNE KHTAKSFRHR IVSSADVSL RPPQFYLSNE KVSAGEAAVV QKRAEATDSY GEQLDEISAR WAAKFYGRVT FGPRNYPYPS SRWLARRFKM K KHRIIKRF RFRRYKLAAV ANLPFAKMIR VGLLPELKSS KTKKGDDVEA DLSGRLVSAV RSSGGKKKGN RGRPKSKYQI

Macromolecule #41: Trafficking protein particle complex subunit

• Macromolecule: Name: Trafficking protein particle complex subunit / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 28.87048 KDa

Sequence

String:

MLRRAVVALR RDALNGGSSM TLGSKGMKLS PDPHRRRMPW TAAKEYVPGV VLNAKEKMVL DGVKLVDVEC VDRASQVDPL EALRATVAT YEYNTSTGKN IFQLASQVEF DGRGQRFYRK EWQEGTYDKY VTLSAIDFNR DGNKGTAYGY VTFHGETTTR P VQIDFADV PGWHMEFRVE RAVPFNAIVP PPPSIGTDVP VDPRSYRLRA YPFYDAPNPP EFVERLLKDR GVIPDSPVET TE LPNESEG SDDTTRRKN

Macromolecule #42: mS59

• Macromolecule: Name: mS59 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 35.605117 KDa

Sequence

String:

MMRCCCVLQD KSMFAAKRRV IVPIHPTPNY PAHFIKASFT TDPLKEKQKA RFSSGGEAMR EVQMIPKNLE GERSRRELMS RGDTEFEAL VEFIQGASYD QLISGRRFKK VYDKLSENDD TFVWLCHTAM SVLNPGDVRS RLVYNHLRTL AEAVANGEMT L RTAFRFYE SAVRSPAYRE IAKRQMEGGA ATRLAGISAA ADVMRRMGLT RRPMASYFEL YQRIVERSEA MTPWGFPPLF QF EERLSLE PRLKFFSRAS QQALERRRRG HIMSAYTTLQ GRRIFWIPPT WNRAGRFLGP HVTLYPGMTP D

Macromolecule #43: mS26

• Macromolecule: Name: mS26 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 51.718258 KDa

Sequence

String:

MMRCSRGCRR QLRPYYNLPS KSDHGRKMTG FLTPYRHWMW KQNELWRNVH EAQFEHLRKV YKRQWLESFR VNADEYIYKY NITKAAQLA QWEYEMQAQE KKRLEAKQMT EGRQALKKKH LDLLREFHER QFFFWYERAS ERLQSMNLIQ YIPQSRVQEH I ERELDKYV AGKSEAYPLN FVGQMPLVED REGNIVEVPE GLLTNHVSEH PESTVKPHQP HESTSVSVEE QLLRTMASAR EE SLEEWID DSRALSETID DISREEEQRD EDTRVARSME ETDNEREISR RMYIDRGKTG SKAIFRRPTL SETDGGSASI PVG GVAASP ADTSAPMRRR KKGKLDKAHA LQEQQDAMIA RMSAKSLKDG ESSISIVKRG EIATSRGRIR DKAAIPTQEV LMQK PELAA GSVPNARISF KDKVDQLYHR GKYKQKKEDD NNPNEDL

Macromolecule #44: mS23

• Macromolecule: Name: mS23 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 34.86916 KDa

Sequence

String:

MRSTALRLYK MPKNMGVAPR FDVWNESYEP WQHMKRMGRL VGTGFYIPPE WYNHFRMFPP INHNFQQEKT LNPRNESEPT QDDTGSLSQ ERAALRDELA RKSRLLASEG MRYYNIFWVR KPLDRMEKEY YELKRKGIAH SEAIKKVLEG FYKELAVKKR V AAVQAEEA KLSGRFITMR EATVVLNVLA QLHREQLTPH QVTLLAREQH QATEKASGLT ATVSRVSAPA AGIDADSTAS KE SGSDEAL SADSLANMLE DDHASGAGTQ YQVEVKHSAR DSVRQLHEKS TDDTGSPDWY TGASPVYNGA A

Macromolecule #45: mS22

• Macromolecule: Name: mS22 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 73.123039 KDa

Sequence

String:

MFRRGLVHRR YPFNKRGPRE RKSWKHHVLT DPPKPIQWRD PKVWTKDLTT MKSFDAPQWD LWQSRARSED IDEALQPFMD MPQSLKDRR YDIPWWANPF GAWYLQNILS VELLKLPSRT NAEKVAIYRN QKHSLSSKKK GEAAQDDEIL ANIIKERWRT L EFGDRDAG YPCTFSDYIQ FLNEWFKSLD EEGMQRLREH FDRRIRPLLA VMSPVDILWL EALTQNSPHN KEQLQRRIAF QT SLGTPEF FDMSKRLRYE INEDYKVRDE LGPELFALWS KAPERWPPER LSKMYGLDFT LVRKILVWHH FKACYDACVE PDW TLPKRL FALEWIRDVR ARKHGLFYGK MRFAEQKITF YSDRFLFRDL VNRREASYAN VWEMDDPYRF LQTEQDYEDY WGDN YDVYR RMFPEMIGKS GEPVQQYGQM PVWTGPHRQH ANKSQHNWMF AEIGVNVGHE ALKKLELDPT NEKRRRFVIR QPDGT LRSA KMSEMRAWYW KEEWADFRFW APNMEWGIEN TPSQEQYQEH VPDTPDADFR KQRRIQSRPV KWFYESHYTR TGNFAG FQP LRFMQRRTER EVRWPDVINA AVQIQKRKPD AYIFKAIPEI

Macromolecule #46: bS21m

• Macromolecule: Name: bS21m / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 23.030959 KDa

Sequence

String:

MLRITSACRG GYMMYHRKSM GTMKYSKWKG AHGGVSHFYG RTPMVEEVKR NEPITLIDRR IMHYVHRSRL RHFQLFRSYQ QKSNATECK LREGEMLRRR WHRKLQKSFI AFMQFKTMKV LEDQAKLVNT YGQAAVNAAL GDSWDAVSDK EKDRKYVTIR R QVKALPVV SVVPKHVATM KQIHNDRFNY RWRVN

Macromolecule #47: bS16m

• Macromolecule: Name: bS16m / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 22.422164 KDa

Sequence

String:

MLHFTSLFRA RAIIKRRTPQ LWGAPGAPII RMRGHHVVWK FQSYDLFVEH THKRRNSDAR LLHYLGKHCP HPQKSLWSPD TPVAQDRHL FMLTTVDVDA FKYWFGVKRC RLSMRPWALL AKAGLLPPSL RQNSKIMPKP IFDKEQLMRY YLANRKEEAT I EREDYLNY KNSLVKSEEE RAAERPVAPY L

Macromolecule #48: bS6m

• Macromolecule: Name: bS6m / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 19.189016 KDa

Sequence

String:

MVFYAFVLVM KPRQRRFTSQ ALREIAVAIY NNGGLIRKLS NEGVMRPYSR FRDADNTPLT YARYVTLQVD MGEEQMRKVE KIMKEHQDV FITLNLNNLE RPVGIRRGRK DLQSAYFPLD TFTRLEEEIN WPPQTSADIY KQLEMNWKEF SRTRWSNFLR N

Macromolecule #49: MURF5

• Macromolecule: Name: MURF5 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 10.806988 KDa

Sequence

String:

MTKKGATKIL FIYKLSKLNV YNNESYKIKL LFNHLYCIDN YNSIYFNLNG ILIWLNVLHI NIILIKYAFL ILLNNLEYLI IFKYNIISI K

Macromolecule #50: Ribosomal_S5_C domain-containing protein

• Macromolecule: Name: Ribosomal_S5_C domain-containing protein / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 49.82309 KDa

Sequence

String:

MFHFTVGSLK NLNSWYAKGT MRGGVPRIYY AWMRPGSFTR RRFEKMRNPF VDLETGTSLY FRDTRDSAEA VAHAADSKGL KGMDNAIDL YNEYRIVPDL YPEGFQWKHK LNTEYNQWRS NTWLTPDLIP QEHRGRFLCN FQLNIVAYDM RVVKFSPKDH R QWIYCVLY VGSGKGIAGW GRAVAPSTQE AKKEAIREAF SNIIAVDLEQ EGPMYPVRVN ADGVRVLLYP AKRIVANFRV AD ILCAFGF QHAGCRINLK ATNNPKSPTH TVEGVFEAVK ALRSVSEIAA SRGKVPHSLI YNIYPYLEEI RRRKGMMAMH PPG KDGLLM PDRVVDNRLP DHLKKGYYDD VYWKDFFAGS REHLNEPKMG LRGDEMRQRL ESAQSRPISS STGSGRRTLE DVLK RLGKT TKDLGSIPIV NPRLDIKLPT HIKRNYSLH

Macromolecule #51: mt-iF3

• Macromolecule: Name: mt-iF3 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 42.914078 KDa

Sequence

String:

MKKMVFCKMC RPLLVFCATS CWRRSARLPL PFFFSPLNLQ VAPFTSWNLQ ARYFSTTAGG GREGTNSSED DYVFDPTLSV QKDAAIHVA KKSLDAIVRD LLPENAPDAA TQKVRAYLQQ HPMDTLITQP TVHITHVEDP ESGRETKMSL SPCDLSEALE Q AQEREMNL VQMGTRGDVA YCRIRREIPR ILGLVGPELE ALREEEKQEG SSHRGGSDQA GGKIRELVDH SFRDVVDAHF VG WKSKKIV EDIKKRHPVK ITIKEFQSPE AAIGKIREMC QAMQRYAEEK LIYHHFTSIV ANDREVSVSF VPSLPSEKGN SWK HIKYPG EKEWAHANKR MEEACRKSGR YGTYVKNNML KPRSLGQTFF RVDKYGRKID

Macromolecule #52: mS48

• Macromolecule: Name: mS48 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 206.534984 KDa

Sequence

String:

MYAHVVSWTL FLHVFFFFLS LSRTLFFFFF FFVCLFFPFL CVYLEEEMLR RLVSSHGCSN GGGTNGGRCV RPVKEEPPVF SSLVLQRRF SFKYATKLQH DEMRQPFYIH EKRHGIFSNE KNIRKSRRGL PFITPLYTRH MNLWETDTDA SKNRFFRGYV F GQRELHQL LGRPHGFEAN NTDGSNDISA YEMTTDQRYK GIPRPAITNL HYEPEWNYTL YRAGTHGSQL SNPRSPLTAE VL GDELMKI RDIKSFDHCK AWFDRLQYLI KLHYDAVGDI GEFKSRHTQH VHEFFVAFHD ALSSFDFGDS YLFEQFHAAR PSE LTDLFG IFLEMEANYV HEDYCPRCSL PYSTTRYCGE GDANTPFRKH RGRWAPHQRW GREWYAVVAR RAEALWYRAT EDPY FGTPQ HTQRQAEALL RVYVQTKQRG KAIDFMNALR GSKEFLLGSI CITPEMQESY DRLLDTTPHP HLLTNGFTLE SNAAK YTGE VQKVPFSPLQ FRIDMEMNKY RRQQKEEGAV RVPPAMWRID TSAIVPYKVD PKTKRVINWR EVKEGIEKSF LSTGLP KEA YTGSEWREML HLKSIIAGRA AKAAELERRL HTDKVKLLEV SSKSKIASET NTSTGSSNSS SIIFPDKDGY HVFDTSV AS LRPFGVSQSG AVFQSVTHTY PSPHAVLYND PVHGKQFILD TTNESCHLFG GFEHGDRLLI RARKIDKNDN NPNSVLAV K GNEFEVIVVG VNKEGSDSEW QLCAMHVDPA LQREYGLVFL GTDCVDIHER WASVRYAAAA PHVKGRVTLL EERRTAREE SLGQIVGVRD GVLFVQWRLL RGGGSEMDRS VAEPIGTVEQ VRNAYQITEQ GVEELMHPPS WRTPFRNDFA EERLEELRQA PFKRENWVS LIQGRYTPKL KRFGYTQHTT MDDFETKEYK DRLLSKQFFH NPQAFEVIPD RRDRAVTFGG KWEYQRTHGL P TVDRNELE NGWSEVEAVT DAEMHVIEQA LRDISGRRPG NFIKSPTKKN TLQLNESWWE PLEFGWEQHN KEQKALVDPT EQ RLIDSAS LPFGGKIPPF GTTIGIGERI REIAEDYAKG FGLGPHGHSP SHDTCQYNTL NAEEDRVREL GYKDALVRLF DEK MADKDV HQWAVEQCAD GEADVRQLLL SLHEWRERGR PPSLMLLQVL SKYLEQEIAA FNEGVPSSVP KLSLQTVDGT LSPS GNSGE RSGTIWADVE PTAYALQYAS QANHSSLDEP FILQLLKSAQ LGGRNAQFTD PFYNAYLENS VVSEFQLGLA ALAGK GVSP SLLAQKISQL HRGSVRLSGN VIPFVKSREL AHLLERMGLS SENIAVVTRG LANCPEQESV GDDFAVPVSV ILSWGG PGS GSSTNAADRK GNATQSRNEL QRKGSAALSS AIRQLGQKRS SSKNWQNEDK MMVHVIEELA LRDDGLVMDI QYIVREN RR NPVLRHEFFA ALLPVFAGKH EKVAQLYDEY CEGKYVPNIT LAIEAFIAFL CNVTKHADVY PGSSYFDVDT TNGPNAGQ Y ISLKLLDPLD GPFIFDNIKA EHIETVERFK QHGIQVGPVR APATGFIAAN SKSLSYFTRR PEEVVYVSTD ADQGLRRSL ERSAHYKTIA ASPAMQFLLH TQNGAGLVAT FNRFFYRTMP MLSFYQRILK HYSDNVQPLR QKAQNSVRGL ARVLENERSA AMEEFRRNS ERYWRNVLEG RSVEQAMGGS GGSGGGGGGT TPPVSSSPSQ SSQEAMAADV ARAIGSGRTG DQKGGAARQQ Q QQQQRTAG DDGGVRTTFA SRKGGSRSMT DLLSKLNKPK GSNTSGTAKG PTKRGNPKSH TDGGRGAKP

Macromolecule #53: 30S Ribosomal protein S17-like protein

• Macromolecule: Name: 30S Ribosomal protein S17-like protein / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (eukaryote)
• Molecular weight: Theoretical: 35.84091 KDa

Sequence

String:

MLRRSLVTFP WWNFQTEHRQ RCVMMYGGSR AKNTHNANHR VFIKKYKRNA FPNRTRHHWA VSMTGVLGQR PRRMPWPYDL TSLIFNQPR QGSDKIGYVV GTSMLKTAVV AANHMVYYPK FNQRVSRTSR FFAHDEDLAC VEGDLVHIKQ CRKISKYKHY Y VFSILEPN IEGRERLKLG LKAVPPPLFG YPVSRRIVKL NLTNAEGTKE KLAAVIQEHV QDAYRFAGPT PDQPRNRLTD SV TFDDANN MIAPNAPATP ESLEGGESPS LLGDKEEYTE VEQDTRNKKG DEYWMNLQPK EKYDFKGFKK SP

Macromolecule #54: Ribosome protein S12

• Macromolecule: Name: Ribosome protein S12 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (eukaryote)
• Molecular weight: Theoretical: 9.806853 KDa

Sequence

String:

MWFLYGCCLR FVLFVLCYYM SPRLPSSGNR RVLYAVFYLY IFVWCVRCFF CGIFILSFAL FIIEGGGFID LPGIKYYTRI CY

Macromolecule #55: mS65

• Macromolecule: Name: mS65 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 29.683416 KDa

Sequence

String:

MFGRSALCLA KRFRYNTKYP SLVSYNKLPW EILNHETPEF HMHVAPHYEQ IMTLAASTHV PHIVGKKHLE MPPEHRLRLL PGMFYMLDG DSIPEGFTAN RVLDPTALQY YGRLESLVAP VQAVRMLISD DLRIVCNSVT LQGPLQLPVA SYASLASLEV V TNKASASF TLFHFVRPNR PPSELQLEKY YIHAPRAMAL AEFNSTSNTS WEPKLQAPKR SKRVTPLPAY RPPQSYLMGL AE RLAVVPG SSFGRRSLMW GHWF

Macromolecule #56: RNA (478-MER)

• Macromolecule: Name: RNA (478-MER) / type: rna / ID: 56 / Number of copies: 2
• Source (natural): Organism: Trypanosoma cruzi (strain CL Brener) (eukaryote) / Strain: CL Brener
• Molecular weight: Theoretical: 2.61641425 MDa

Sequence

String:

UUUAAUGGGU AAUUUUAAAG CAAGUAAUUA UGAAUUAGGA UAAGAACAGA AUUCCUCAAG UCCCUAAUUG CGAUUAUUUG UUAAGAUCU UUUUGAGGAU AGAUCUAAAA UUACCAAGUC CAAUUUUUGU AUAUGGGCGG GCUAUGAAAA UAUAAAAUUA U AUAUUUUC UAGUUUGAUC GAAAAUGCUU UUCGAUUUGA AAAUUUAAAU UAAAUUUAAG UUUAAUUUUC AAUUUUCAAA AU UUGAAAC AAUUUUGGAA UUUUGGUAGG UAUUUUAUUG AUAGGUUUAA AUCACCGCUG UAUAAAUUUU GGUAGUAAAA CUU UUUGUA AUAAUGCGUU UUUAUUAUCA GUUAUUUAUG GGUGUUUGUG AUUUAAAUGU AAUCAGUUUA GUACAAAUCA UUUU UCUAA AUUAUUUUGA GUUUUGGGAU UUGGAGGUUU GAACUUGAAU UUAAAUUUAG UUUCAAGUCA AGUCGUAUAA AAAAC AUGG CAUUUUUUGU UGCUAUAAGU UUUUUAUAUA ACUCUUUGAU UCGAAAUUAA AUUUAAAUUU AGGUUUUAGC UAUUUU AAA UUCCAACUUG AAAUUUGUUU UGGGUUUUUA UAAUUGAGUU UUAAAUUUUA AAUCCAAAUU UAAAUAGGAU CUUCUUU AC UAAUGAAAAU AUUUUACAAA UCUUUUGCAA AAAUAUUUUA AUUUAGUAAG GAUGGUUGGU AUUUUAAAUU UCGGUUUA A UUUUUAAAAU UUUUUUAUUG ACCAAACAUU UUCAAGGUUA GUGGGAAUAG CUAUGACUUU GGUUUAGAUU UAGUUUUAU CAUUGAAUUG UUAUGUAAAG GAUUUGUGGU UAUACAAUAU GUUUAUGUAU GUGUUUAUUA UAUGUACUCG AUUAGAGAAG CUAAACUUA AAUUCAAACC UCCAAUUUCC AAAACUUGAA ACAAUUUUUA GGUGAUUUAU UAAGAAUUGA UUUAAAAUUA U GAAUGUAU AAAUUUUGGU AGUAGGUUUU UUUUGUAAUA AUGUGUUUAU AAAUUGUAAC UAAUCUGGUU UAAACUAUUU UU CUAAAUU AUUUUAGGUU UUUUUUGGGA CAUGAGAGUU UAAAUUUGAA UUUACUUUUA AGUUAUCAAU AAAAAACAUG UUU UUUGUG CUAUUAAAAU UUAUAUAAUC UUUUUGACGU CAAAUUUAAA UUUAGGUUUA UUCUAAUUCG AAACUUUUUG GUUU UUUAA UAAAUAACUC CAAUAAAUCU AAAUUUUUUU AUAGAUCAAA CAUUUUUAAG GUUGGUAGGC AUAGUUAUGA CUUUC UAGU UUAAUUUAGU UUUAUUUAUU GAAUUGUUAU GUAAAGGAUU UGUGGUUGGG AAUGUUUAUG UUUAUGUUUA UUAUGU GUA UUUUAUUUAA UUAGAAAAGC UUUUAAAAAU UUAAAAUUUG UAAUCCAAAU UUUACCAAUU AAGAAGAAUA UUAUAAU AA UGGGUGUCUU AUAUUUUAAA UAAAUAUUUA AAUUCCGUGU AGUAAAUUUA UUAUUUGUAU UAUUUAUAUA AUAGGUGU A UUAUAUUUAA AUUUUAAAUU UGUUGUUUUA UAUUUAGAUA CAUAUUUAUA GAUUAAUAUA UUUAAAUAAU AUUUUAAAA UUUAUUGAAC UGUAAUUAUU AGUUUAAUAU UUUUAGUUUG AUGUUGAAAU AUUUAAUUAA AGAUGUUACA GUUGUUCUAU AUGUACCAA AUAAAUAUAG UAAGAUUAUU UUAGUUGAAU UAAUAAAUAA AUAUUUAUUU UUCUUUGUAA AUAUUAUGAA C AAUUUAAA AAUUAAUCUG UUUAACUAAA AUGUUAUAUA UAAUAAUCUA AGUUAAUUUG AAUAUUAAAA GUACAAGUAU AA UUUGUAA UUCUAAAGUA UUUUAAUGGU AUAUUUUUAG UAGGUAAAUG AAAAGUAUAA AUGGAUAUAA CUUAAUAUUU AAU AUUUGU UUAAUGAAAA GUAUUUUAUU AUUAUAUUGU AUAGUAUUAU UAUAGUGUAU AGUUUUUUAA AAAUAUAAAA AUAU UGUUA AUAAAAUUAU CGUAUUUUAA GUGCGUUUAU UAAAUGCGUU UGUCUAAGAU AAUUAUUUAA GAUUAUUCUU GUAAA UAUA UUUAAAUAUU AAUAAUUCUU AAAAUAAAAA AAUAUCCUCA AUUGCAAUAU UAUUGUAGCA UAGUAAUUUG UUAACU AAA UAUUAAAGUG UUCCAUAGAA AAUUUUUAAA UUACAACAAA UAAAAUAAAG UAUGAAUUAA UAUCAAAAUU UUAAUAA AA AUUAAAAAAU UAAAAUAGGG CAAGUCCUAC UCUCCUUUAC AAAGAGAACA UUAUGAUAUG UAAUUGUAUG UUUGAUUG G GGCAAUACUA UAUUUAUUUA UAUAGCAUAA GAACUAUAUU CUUUGAAAUU AUAAAAGGUU CGAGCAGGUU AACAAGCAU UAAAAAUAAA UGUGUUUCAU CGUCUACUUA UUACCAUGAU UGAUUGUUCA UCAAAAUAGU AAUUCGUUAG UUGGGUUAAA AUCGUUGUA AAGCAGAUUU GUUUAUAUAU UUAAUUUUUA UAAUUAAUAA UAAUUAAUAU AAGUACGCAA GGAUUGAUUA U UGAAAAAA GAAAGAAGAA UAUAAUUUAU AUAAAUUAUG GUCAAUUGUU AGUAUUCAUA UUAAUUUUUU UAAAUGUUUU AU CAUUUUA UAAAGGUUUA UUUUUGAAAG AUUUUUUGUA UAAAAUUUUA GGAAUAGUUA 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AUAAAAAAA UAGUAUAUAA UAAUAAGUAA UACUAAACUU AUACUAUAAA UUAAGUGAAA AUUUAAAUAU AAAUAAAAGA UAUAAUUUUU UGUUGAAAU AAAUAUUAGG AAUAAAAAGC AAAAAUUAUU CACACUUAAC ACAAAUAGUA AACUAACGAU AGCAAAGCUG U UUAAUCCA AUUAAAACGC AUGUACAAGA UUGAAAUAAU AGAAGUUUGA UGAAUAAAAU AUAAAAAUAA AUGAAGCUAA UU AGUAGAA UUAUUAAUAU AAAACAAAAC AAAAUAUAAA AAGUUAACAU AUAAAUAAAA AUAAAGACAC CAAGUCUAAU AUA AAGUUG CUCCAUAAAC AAAAUUAAAA AGGCGAUGUA UAAUUUGAAU AAAAUUAAUA AUGUGUAAAA UAGGCAUAAA AUUC CAAGU CAUUCUUCAU CAAAAACUAA AAAACAAAAA UCACAUAGGA AAAAACAGUA GUUUAAUAUC AUAAAAUAUA AUAAU AUAA AUAAUAAUAU AAAAUUUAUU AAGUUUAACA UGUAGUAAUA UCAUAGAACU AAAAUUUUAU AUCCAAAUCU ACUGGA CAU UAAUAAUAAA AAGAGCAAUA AGCUAAAUAU UUCAAAGAGG AUUGAUAUAA UAAUAAUAUG AUUAAUAAAU AUAAAUA AG AAUAUAAUAA UGUAUUGAAU AAUAAUAAUA AUGAAUAAAA AUCUGGUAUC GAAUGAUAGA AAGCAAAAAA AUAAUGUA A AGCAAAAUAA GAAUAAGAGU AUAAAGAUGA AACAAAUAUA AGAAUCUAAU AAUGUUAUUC AAAAUAGGUU AAUAAUUAA UAAUCAGAGU AAAUCAAAGC UUAGUAAUGU UAGUGUAGUA UAAUCACAUA AGAUAAUAAA GCUGUAGAUA AUAAGAAAUA UAAAUAUGU GUAUGAUAUA UAAAAACAAG GAUUUUUUGG GGGUUUAGGG

Macromolecule #57: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 57 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Macromolecule #58: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 58 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #59: URIDINE 5'-TRIPHOSPHATE

• Macromolecule: Name: URIDINE 5'-TRIPHOSPHATE / type: ligand / ID: 59 / Number of copies: 1 / Formula: UTP
• Molecular weight: Theoretical: 484.141 Da

Chemical component information

ChemComp-UTP:
URIDINE 5'-TRIPHOSPHATE / UTP*YM / Uridine triphosphate

Macromolecule #60: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 60 / Number of copies: 4 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TALOS ARCTICA
• Electron beam: Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 60.0 e/Ų
• Experimental equipment: Model: Talos Arctica / Image courtesy: FEI Company

Image processing

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 148180