9I53

Crystal structure of the SARS-CoV-2 helicase NSP13 in complex with ATP

Summary for 9I53

• Entry DOI: 10.2210/pdb9i53/pdb
• Related: 9I51
• Descriptor: SARS-CoV-2 helicase NSP13, ZINC ION, ADENOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• Functional Keywords: nsp13, helicase, sars-cov-2, hydrolase
• Biological source: Severe acute respiratory syndrome coronavirus 2
• Total number of polymer chains: 2
• Total formula weight: 136915.05

Authors

Kloskowski, P.,Neumann, P.,Ficner, R. (deposition date: 2025-01-27, release date: 2025-07-23, Last modification date: 2025-08-13)

Primary citation

Kloskowski, P.,Neumann, P.,Berndt, A.,Ficner, R.
Nucleotide-bound crystal structures of the SARS-CoV-2 helicase NSP13.
Acta Crystallogr.,Sect.F, 81:338-347, 2025

PubMed Abstract: Nucleotide-bound crystal structures of SARS-CoV-2 NSP13 in ADP- and ATP-bound states were resolved to 1.8 and 1.9 Å, respectively. The ADP-bound model captures a state immediately following ATP hydrolysis, with both ADP and orthophosphate still present in the active site. Further comparative analysis revealed that crystal packing influences NSP13 by stabilizing the nucleotide-binding site, underscoring the importance of accounting for these effects in structure-based drug design targeting NSP13.

PubMed: 40638074
DOI: 10.1107/S2053230X25005266

Experimental method

X-RAY DIFFRACTION (1.92 Å)