8UB6

Crystal Structure of a reconstructed Kaede-type Red Fluorescent Protein, LEA H62X, containing 3-methylhistidine at position 62

Summary for 8UB6

• Entry DOI: 10.2210/pdb8ub6/pdb
• Related: 8THS
• Descriptor: LEAST EVOLVED ANCESTOR (LEA) GFP-LIKE PROTEINS (2 entities in total)
• Functional Keywords: beta barrel, luminescent protein
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 26525.22

Authors

Henderson, J.N.,Mills, J.H. (deposition date: 2023-09-22, release date: 2023-11-29, Last modification date: 2024-01-10)

Primary citation

Krueger, T.D.,Henderson, J.N.,Breen, I.L.,Zhu, L.,Wachter, R.M.,Mills, J.H.,Fang, C.
Capturing excited-state structural snapshots of evolutionary green-to-red photochromic fluorescent proteins.
Front Chem, 11:1328081-1328081, 2023

PubMed Abstract: Photochromic fluorescent proteins (FPs) have proved to be indispensable luminous probes for sophisticated and advanced bioimaging techniques. Among them, an interplay between photoswitching and photoconversion has only been observed in a limited subset of Kaede-like FPs that show potential for discovering the key mechanistic steps during green-to-red photoconversion. Various spectroscopic techniques including femtosecond stimulated Raman spectroscopy (FSRS), X-ray crystallography, and femtosecond transient absorption were employed on a set of five related FPs with varying photoconversion and photoswitching efficiencies. A 3-methyl-histidine chromophore derivative, incorporated through amber suppression using orthogonal aminoacyl tRNA synthetase/tRNA pairs, displays more dynamic photoswitching but greatly reduced photoconversion the least-evolved ancestor (LEA). Excitation-dependent measurements of the green anionic chromophore reveal that the varying photoswitching efficiencies arise from both the initial transient dynamics of the bright state and the final -like photoswitched state, with an exocyclic bridge H-rocking motion playing an active role during the excited-state energy dissipation. This investigation establishes a close-knit feedback loop between spectroscopic characterization and protein engineering, which may be especially beneficial to develop more versatile FPs with targeted mutations and enhanced functionalities, such as photoconvertible FPs that also feature photoswitching properties.

PubMed: 38144887
DOI: 10.3389/fchem.2023.1328081

Experimental method

X-RAY DIFFRACTION (1.7 Å)