8OZS

Populus tremula stable protein 1 with N-terminal binding peptide extension with hemin

Summary for 8OZS

• Entry DOI: 10.2210/pdb8ozs/pdb
• Descriptor: Stable protein 1 (2 entities in total)
• Functional Keywords: hemine, protein engineering, biohybrid, catalysis, plant protein
• Biological source: Populus tremula
• Total number of polymer chains: 24
• Total formula weight: 328352.78

Authors

Sklyar, J.,Zeibaq, Y.,Bachar, O.,Yehezkeli, O.,Adir, N. (deposition date: 2023-05-09, release date: 2024-03-27, Last modification date: 2025-04-23)

Primary citation

Zeibaq, Y.,Bachar, O.,Sklyar, J.,Adir, N.,Yehezkeli, O.
A Bioengineered Stable Protein 1-Hemin Complex with Enhanced Peroxidase-Like Catalytic Properties.
Small Sci, 4:2400025-2400025, 2024

PubMed Abstract: Enzymes have gained their unique efficiency and catalytic activity through billions of years of evolution, perfecting their active site to a desired reaction. Inspired by nature, a novel enzyme-mimicking platform is designed based on stable protein 1 (SP1) to create a nano-compartment that mimics peroxidase activity. The biohybrid reveals enhanced activity over the hemin cofactor alone and improved stability in organic solvents in comparison to native peroxidase. Furthermore, the utilization of the obtained biohybrid in an optical glucose biosensing platform is shown. The biohybrid crystallographic structure is solved, indicating that the SP1 structure is not affected by the hemin coordination. This work opens the path for developing new cofactor binding centers in engineered protein scaffolds for various artificial catalytic processes.

PubMed: 40212758
DOI: 10.1002/smsc.202400025

Experimental method

X-RAY DIFFRACTION (2.4 Å)