7KGO

Crystal Structure of HLA-A*0201in complex with SARS-CoV-2 N351-359

7KGO の概要

• エントリーDOI: 10.2210/pdb7kgo/pdb
• 分子名称: MHC class I antigen, Beta-2-microglobulin, Nucleoprotein, ... (7 entities in total)
• 機能のキーワード: hla-a*0201, t cell, sars-cov-2, covid-19, viral peptide, tcr, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 45306.72

構造登録者

Szeto, C.,Chatzileontiadou, D.S.M.,Riboldi-Tunnicliffe, A.,Gras, S. (登録日: 2020-10-18, 公開日: 2021-01-20, 最終更新日: 2024-11-20)

主引用文献

Szeto, C.,Chatzileontiadou, D.S.M.,Nguyen, A.T.,Sloane, H.,Lobos, C.A.,Jayasinghe, D.,Halim, H.,Smith, C.,Riboldi-Tunnicliffe, A.,Grant, E.J.,Gras, S.
The presentation of SARS-CoV-2 peptides by the common HLA-A * 02:01 molecule.
Iscience, 24:102096-102096, 2021

PubMed Abstract: CD8+ T cells are crucial for anti-viral immunity; however, understanding T cell responses requires the identification of epitopes presented by human leukocyte antigens (HLA). To date, few SARS-CoV-2-specific CD8+ T cell epitopes have been described. Internal viral proteins are typically more conserved than surface proteins and are often the target of CD8+ T cells. Therefore, we have characterized eight peptides derived from the internal SARS-CoV-2 nucleocapsid protein predicted to bind HLA-A02:01, the most common HLA molecule in the global population. We determined not all peptides could form a complex with HLA-A02:01, and the six crystal structures determined revealed that some peptides adopted a mobile conformation. We therefore provide a molecular understanding of SARS-CoV-2 CD8+ T cell epitopes. Furthermore, we show that there is limited pre-existing CD8+ T cell response toward these epitopes in unexposed individuals. Together, these data show that SARS-CoV-2 nucleocapsid might not contain potent epitopes restricted to HLA-A02:01.

PubMed: 33521593
DOI: 10.1016/j.isci.2021.102096

実験手法

X-RAY DIFFRACTION (2.15 Å)