7JZJ

Crystal structure demonstrating CTD-CTD interactions of Zaire Ebola virus VP40 dimer

Summary for 7JZJ

• Entry DOI: 10.2210/pdb7jzj/pdb
• Descriptor: Matrix protein VP40, PENTAETHYLENE GLYCOL (3 entities in total)
• Functional Keywords: vp40, matrix protein, ebola virus, viral protein
• Biological source: Zaire ebolavirus (strain Mayinga-76) (ZEBOV)
• Total number of polymer chains: 4
• Total formula weight: 129631.37

Authors

Norris, M.J.,Bornholdt, Z.A.,Saphire, E.O. (deposition date: 2020-09-02, release date: 2020-10-28, Last modification date: 2024-10-23)

Primary citation

Wan, W.,Clarke, M.,Norris, M.J.,Kolesnikova, L.,Koehler, A.,Bornholdt, Z.A.,Becker, S.,Ollman-Saphire, E.,Briggs, J.A.
Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers.
Elife, 9:-, 2020

PubMed Abstract: Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting that localization to the plasma membrane, oligomerization into a matrix layer, and generation of membrane curvature are intrinsic properties of VP40. There has been no direct information on the structure of VP40 matrix layers within viruses or virus-like particles. We present structures of Ebola and Marburg VP40 matrix layers in intact virus-like particles, and within intact Marburg viruses. VP40 dimers assemble extended chains via C-terminal domain interactions. These chains stack to form 2D matrix lattices below the membrane surface. These lattices form a patchwork assembly across the membrane and suggesting that assembly may begin at multiple points. Our observations define the structure and arrangement of the matrix protein layer that mediates formation of filovirus particles.

PubMed: 33016878
DOI: 10.7554/eLife.59225

Experimental method

X-RAY DIFFRACTION (2.46 Å)