6ZFF
Pyrococcus furiosus Rad50 coiled coils in rod configuration
Summary for 6ZFF
| Entry DOI | 10.2210/pdb6zff/pdb |
| Descriptor | DNA double-strand break repair Rad50 ATPase, ZINC ION (3 entities in total) |
| Functional Keywords | double strand break repair, dna damge response, scaffold, closed coiled coil arms, dna |
| Biological source | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
| Total number of polymer chains | 2 |
| Total formula weight | 32995.38 |
| Authors | Soh, Y.M.,Basquin, J.,Gruber, S. (deposition date: 2020-06-17, release date: 2020-07-08, Last modification date: 2024-05-15) |
| Primary citation | Soh, Y.M.,Basquin, J.,Gruber, S. A rod conformation of the Pyrococcus furiosus Rad50 coiled coil. Proteins, 89:251-255, 2021 Cited by PubMed Abstract: The Rad50-Mre11 nuclease complex plays a vital role in DNA repair in all domains of life. It recognizes and processes DNA double-strand breaks. Rad50 proteins fold into an extended structure with a 20 to 60 nm long coiled coil connecting a globular ABC ATPase domain with a zinc hook dimerization domain. A published structure of an archaeal Rad50 zinc hook shows coiled coils pointing away from each other. Here we present the crystal structure of an alternate conformation displaying co-aligned coiled coils. Archaeal Rad50 may thus switch between rod-shaped and ring-like conformations as recently proposed for a bacterial homolog. PubMed: 32875643DOI: 10.1002/prot.26005 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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