Structure of Clostridium butyricum Argonaute bound to a guide DNA (5' deoxycytidine) and a 19-mer target DNA

Summary for 6QZK

DescriptorClostridium butyricum Argonaute, siDNA guide (5'-D(P*CP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*TP*AP*GP*T)-3'), DNA target (5'-D(T*AP*TP*AP*CP*AP*AP*CP*CP*TP*AP*CP*TP*AP*CP*CP*TP*CP*T)-3'), ... (6 entities in total)
Functional Keywordsargonaute, pago, ago, cbago, hydrolase
Biological sourceClostridium butyricum
Total number of polymer chains3
Total molecular weight98338.4
Swarts, D.C.,Jinek, M.,Hegge, J.W.,Van der Oost, J. (deposition date: 2019-03-11, release date: 2019-04-24, Last modification date: 2019-05-22)
Primary citation
Hegge, J.W.,Swarts, D.C.,Chandradoss, S.D.,Cui, T.J.,Kneppers, J.,Jinek, M.,Joo, C.,van der Oost, J.
DNA-guided DNA cleavage at moderate temperatures by Clostridium butyricum Argonaute.
Nucleic Acids Res., 2019
PubMed: 31069393 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gkz306
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.277170 1.8% 5.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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