6NL3

Solution structure of human Coa6

Summary for 6NL3

• Entry DOI: 10.2210/pdb6nl3/pdb
• NMR Information: BMRB: 30556
• Descriptor: Cytochrome c oxidase assembly factor 6 homolog (1 entity in total)
• Functional Keywords: mitochondrial proteins, oxidoreductase
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 9586.77

Authors

Naik, M.T.,Soma, S.,Gohil, V. (deposition date: 2019-01-07, release date: 2019-11-20, Last modification date: 2024-10-16)

Primary citation

Soma, S.,Morgada, M.N.,Naik, M.T.,Boulet, A.,Roesler, A.A.,Dziuba, N.,Ghosh, A.,Yu, Q.,Lindahl, P.A.,Ames, J.B.,Leary, S.C.,Vila, A.J.,Gohil, V.M.
COA6 Is Structurally Tuned to Function as a Thiol-Disulfide Oxidoreductase in Copper Delivery to Mitochondrial Cytochrome c Oxidase.
Cell Rep, 29:4114-4126.e5, 2019

PubMed Abstract: In eukaryotes, cellular respiration is driven by mitochondrial cytochrome c oxidase (CcO), an enzyme complex that requires copper cofactors for its catalytic activity. Insertion of copper into its catalytically active subunits, including COX2, is a complex process that requires metallochaperones and redox proteins including SCO1, SCO2, and COA6, a recently discovered protein whose molecular function is unknown. To uncover the molecular mechanism by which COA6 and SCO proteins mediate copper delivery to COX2, we have solved the solution structure of COA6, which reveals a coiled-coil-helix-coiled-coil-helix domain typical of redox-active proteins found in the mitochondrial inter-membrane space. Accordingly, we demonstrate that COA6 can reduce the copper-coordinating disulfides of its client proteins, SCO1 and COX2, allowing for copper binding. Finally, our determination of the interaction surfaces and reduction potentials of COA6 and its client proteins provides a mechanism of how metallochaperone and disulfide reductase activities are coordinated to deliver copper to CcO.

PubMed: 31851937
DOI: 10.1016/j.celrep.2019.11.054

Experimental method

SOLUTION NMR