6NEE

Crystal structure of a reconstructed ancestor of Triosephosphate isomerase from eukaryotes

Summary for 6NEE

DescriptorTRIOSEPHOSPHATE ISOMERASE, PHOSPHOGLYCOLOHYDROXAMIC ACID (3 entities in total)
Functional Keywordsisomerase, glycolisis, tim barrel, ancestral sequence reconstruction
Biological sourcesynthetic construct
Total number of polymer chains2
Total molecular weight55419.46
Authors
Rodriguez-Romero, A.,Schulte-Sasse, M.,Fernandez-Velasco, D.A. (deposition date: 2018-12-17, release date: 2019-01-09)
Primary citation
Schulte-Sasse, M.,Pardo-Avila, F.,Pulido-Mayoral, N.O.,Vazquez-Lobo, A.,Costas, M.,Garcia-Hernandez, E.,Rodriguez-Romero, A.,Fernandez-Velasco, D.A.
Structural, thermodynamic and catalytic characterization of an ancestral triosephosphate isomerase reveal early evolutionary coupling between monomer association and function.
FEBS J., 2018
PubMed: 30589511 (PDB entries with the same primary citation)
DOI: 10.1111/febs.14741
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.9 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2013 0.2% 0.8% 1.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6nee
no rotation
Molmil generated image of 6nee
rotated about x axis by 90°
Molmil generated image of 6nee
rotated about y axis by 90°
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