6N61

Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA and Capistruin

Summary for 6N61

DescriptorDNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (13 entities in total)
Functional Keywordscapistruin, complex, inhibitor, lasso peptide, rna polymerase, transferase-dna complex, transferase/dna
Biological sourceEscherichia coli
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Total number of polymer chains9
Total molecular weight458573.02
Authors
Braffman, N.,Hauver, J.,Campbell, E.A.,Darst, S.A. (deposition date: 2018-11-24, release date: 2019-01-09)
Primary citation
Braffman, N.,Piscotta, F.J.,Hauver, J.,Campbell, E.A.,Link, A.J.,Darst, S.A.
Structural mechanism of transcription inhibition by Lasso Peptides Microcin J25 and Capistruin
Proc.Natl.Acad.Sci.Usa, 2019
DOI: 10.1073/pnas.1817352116
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.253 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.31823 0.4% 10.3% 3.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6n61
no rotation
Molmil generated image of 6n61
rotated about x axis by 90°
Molmil generated image of 6n61
rotated about y axis by 90°
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