6MWQ

Single particle cryoEM structure of a DARPin-aldolase platform in complex with GFP

Summary for 6MWQ

EMDB information9277
DescriptorDARPin, Muscle-type aldolase chimeric fusion, Green fluorescent protein (2 entities in total)
Functional Keywordssynthetic construct, platform, single particle cryoem, small protein display, lyase-fluorescent protein complex, lyase/fluorescent protein
Biological sourcesynthetic construct
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Total number of polymer chains8
Total molecular weight314348.52
Authors
Weaver, S.J.,Yao, Q. (deposition date: 2018-10-30, release date: 2018-11-21)
Primary citation
Yao, Q.,Weaver, S.J.,Mock, J.Y.,Jensen, G.J.
Fusion of DARPin to aldolase enables visualization of small protein by cryoEM
Biorxiv, 2018
DOI: 10.1101/455063
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
?

Structure validation

ClashscoreRamachandran outliersSidechain outliers4 1.5% 0.1%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

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Molmil generated image of 6mwq
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