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6JD2

Crystal structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) in complex with Mg2+ at pH8.5

Summary for 6JD2
Entry DOI10.2210/pdb6jd2/pdb
DescriptorPutative ketol-acid reductoisomerase 2, BETA-MERCAPTOETHANOL, MAGNESIUM ION, ... (4 entities in total)
Functional Keywordsbi-specific, thermostable, reductoisomerase, magnesium-dependent, dodecamer, knotted protein, isomerase
Biological sourceSaccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
Total number of polymer chains12
Total formula weight448279.18
Authors
Chen, C.Y.,Chang, Y.C.,Lin, K.F.,Huang, C.H.,Lin, B.L.,Ko, T.P.,Hsieh, D.L.,Tsai, M.D. (deposition date: 2019-01-30, release date: 2019-08-14, Last modification date: 2023-11-22)
Primary citationChen, C.Y.,Chang, Y.C.,Lin, B.L.,Lin, K.F.,Huang, C.H.,Hsieh, D.L.,Ko, T.P.,Tsai, M.D.
Use of Cryo-EM To Uncover Structural Bases of pH Effect and Cofactor Bispecificity of Ketol-Acid Reductoisomerase.
J.Am.Chem.Soc., 141:6136-6140, 2019
Cited by
PubMed: 30921515
DOI: 10.1021/jacs.9b01354
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.53 Å)
Structure validation

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