6GAM

Structure of E14Q variant of E. coli hydrogenase-2 (as-isolated enzyme)

Summary for 6GAM

Related6FPI 6FPW 6G7R
DescriptorHydrogenase-2 small chain, Hydrogenase-2 large chain, IRON/SULFUR CLUSTER, ... (8 entities in total)
Functional Keywordsnife hydrogenase, iron sulphur cluster, periplasm, hydrogen, oxidoreductase
Biological sourceEscherichia coli (strain K12)
Total number of polymer chains4
Total molecular weight193039.54
Authors
Carr, S.B.,Armstrong, F.A.,Evans, R.M. (deposition date: 2018-04-11, release date: 2019-02-20)
Primary citation
Evans, R.M.,Ash, P.A.,Beaton, S.E.,Brooke, E.J.,Vincent, K.A.,Carr, S.B.,Armstrong, F.A.
Mechanistic Exploitation of a Self-Repairing, Blocked Proton Transfer Pathway in an O2-Tolerant [NiFe]-Hydrogenase.
J. Am. Chem. Soc., 140:10208-10220, 2018
PubMed: 30070475 (PDB entries with the same primary citation)
DOI: 10.1021/jacs.8b04798
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.4 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.1711 0.1% 0.4% 0.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6gam
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Molmil generated image of 6gam
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Molmil generated image of 6gam
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