6AL1

The NZ-1 Fab complexed with the PDZ tandem fragment of A. aeolicus S2P homolog with the PA12 tag inserted between the residues 181 and 184

Summary for 6AL1

DescriptorPDZ tandem fragment with PA tag insertion, Heavy chain of antigen binding fragment, Fab of NZ-1, Light chain of antigen binding fragment, Fab of NZ-1 (3 entities in total)
Functional Keywordsprotease, signaling protein
Biological sourceAquifex aeolicus VF5
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Total number of polymer chains3
Total molecular weight67706.61
Authors
Tamura, R.,Oi, R.,Kaneko, M.K.,Kato, Y.,Nogi, T. (deposition date: 2018-09-05, release date: 2019-02-13)
Primary citation
Tamura, R.,Oi, R.,Akashi, S.,Kaneko, M.K.,Kato, Y.,Nogi, T.
Application of the NZ-1 Fab as a crystallization chaperone for PA tag-inserted target proteins.
Protein Sci., 2019
PubMed: 30666745 (PDB entries with the same primary citation)
DOI: 10.1002/pro.3580
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.2 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.3193 0.6% 1.1% 2.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6al1
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Molmil generated image of 6al1
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Molmil generated image of 6al1
rotated about y axis by 90°