6GTD
Transient state structure of CRISPR-Cpf1 (Cas12a) I2 conformation
Summary for 6GTD
Entry DOI | 10.2210/pdb6gtd/pdb |
EMDB information | 0062 |
Descriptor | CRISPR-associated endonuclease Cas12a, RNA (28-MER), DNA (5'-D(P*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP*CP*G)-3'), ... (4 entities in total) |
Functional Keywords | crispr genome editing ribonucleoprotein complex, hydrolase |
Biological source | Francisella tularensis subsp. novicida (strain U112) More |
Total number of polymer chains | 4 |
Total formula weight | 203280.74 |
Authors | Montoya, G.,Mesa, P. (deposition date: 2018-06-18, release date: 2018-12-19, Last modification date: 2019-12-18) |
Primary citation | Stella, S.,Mesa, P.,Thomsen, J.,Paul, B.,Alcon, P.,Jensen, S.B.,Saligram, B.,Moses, M.E.,Hatzakis, N.S.,Montoya, G. Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity. Cell, 175:1856-1871.e21, 2018 Cited by PubMed: 30503205DOI: 10.1016/j.cell.2018.10.045 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (4.24 Å) |
Structure validation
Download full validation report