5Y5L

Time-resolved SFX structure of cytochrome P450nor: dark-2 data in the absence of NADH (resting state)

Summary for 5Y5L

• Entry DOI: 10.2210/pdb5y5l/pdb
• Descriptor: NADP nitrous oxide-forming nitric oxide reductase, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• Functional Keywords: metal-binding, oxidoreductase
• Biological source: Fusarium oxysporum (Fusarium vascular wilt)
• Total number of polymer chains: 2
• Total formula weight: 90074.36

Authors

Tosha, T.,Nomura, T.,Nishida, T.,Saeki, N.,Okubayashi, K.,Yamagiwa, R.,Sugahara, M.,Nakane, T.,Yamashita, K.,Hirata, K.,Ueno, G.,Kimura, T.,Hisano, T.,Muramoto, K.,Sawai, H.,Takeda, H.,Mizohata, E.,Yamashita, A.,Kanematsu, Y.,Takano, Y.,Nango, E.,Tanaka, R.,Nureki, O.,Ikemoto, Y.,Murakami, H.,Owada, S.,Tono, K.,Yabashi, M.,Yamamoto, M.,Ago, H.,Iwata, S.,Sugimoto, H.,Shiro, Y.,Kubo, M. (deposition date: 2017-08-09, release date: 2017-12-06, Last modification date: 2023-11-22)

• Primary citation: Tosha, T.,Nomura, T.,Nishida, T.,Saeki, N.,Okubayashi, K.,Yamagiwa, R.,Sugahara, M.,Nakane, T.,Yamashita, K.,Hirata, K.,Ueno, G.,Kimura, T.,Hisano, T.,Muramoto, K.,Sawai, H.,Takeda, H.,Mizohata, E.,Yamashita, A.,Kanematsu, Y.,Takano, Y.,Nango, E.,Tanaka, R.,Nureki, O.,Shoji, O.,Ikemoto, Y.,Murakami, H.,Owada, S.,Tono, K.,Yabashi, M.,Yamamoto, M.,Ago, H.,Iwata, S.,Sugimoto, H.,Shiro, Y.,Kubo, M.; Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate.; Nat Commun, 8:1585-1585, 2017; PubMed: 29147002; DOI: 10.1038/s41467-017-01702-1

Experimental method

X-RAY DIFFRACTION (2.1 Å)