5W6Q

Structural basis for recognition of artificial DNA by an evolved KlenTaq variant

Summary for 5W6Q

• Entry DOI: 10.2210/pdb5w6q/pdb
• Descriptor: DNA polymerase I, thermostable, DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(1W5))-3'), DNA (5'-D(P*GP*(1WA)P*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3'), ... (4 entities in total)
• Functional Keywords: protein-dna, aegis, unnatural base pair, host-guest system, transferase-dna complex, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Thermus aquaticus; More
• Total number of polymer chains: 9
• Total formula weight: 205700.68

Authors

Singh, I.,Georgiadis, M.M. (deposition date: 2017-06-16, release date: 2018-07-18, Last modification date: 2023-10-04)

• Primary citation: Singh, I.,Laos, R.,Hoshika, S.,Benner, S.A.,Georgiadis, M.M.; Snapshots of an evolved DNA polymerase pre- and post-incorporation of an unnatural nucleotide.; Nucleic Acids Res., 46:7977-7988, 2018; PubMed: 29986111; DOI: 10.1093/nar/gky552

Experimental method

X-RAY DIFFRACTION (2.66 Å)