Crystal structure of the Thermus thermophilus 70S ribosome in complex with Klebsazolicin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution

This is a large structure.

Summary for 5W4K

Related PRD IDPRD_002277
Descriptor23S Ribosomal RNA, 5S Ribosomal RNA, 50S ribosomal protein L2, ... (63 entities in total)
Functional Keywordsklebsazolicin, antibiotic, ripps, ribosome inhibitor, 70s ribosome, inhibition of translation, peptidyl transferase center, nascent peptide exit tunnel, ribosome-antibiotic complex, ribosome/antibiotic
Biological sourceEscherichia coli
Total number of polymer chains114
Total molecular weight4576714.26
Primary citation
Metelev, M.,Osterman, I.A.,Ghilarov, D.,Khabibullina, N.F.,Yakimov, A.,Shabalin, K.,Utkina, I.,Travin, D.Y.,Komarova, E.S.,Serebryakova, M.,Artamonova, T.,Khodorkovskii, M.,Konevega, A.L.,Sergiev, P.V.,Severinov, K.,Polikanov, Y.S.
Klebsazolicin inhibits 70S ribosome by obstructing the peptide exit tunnel.
Nat. Chem. Biol., 13:1129-1136, 2017
PubMed: 28846667 (PDB entries with the same primary citation)
DOI: 10.1038/nchembio.2462
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.25281.1%5.7%5.7%0.55MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution