5U08

Crystal structure of an aminoglycoside acetyltransferase meta-AAC0020 from an uncultured soil metagenomic sample in complex with sisomicin

Summary for 5U08

• Entry DOI: 10.2210/pdb5u08/pdb
• Related: 5F46 5F47 5F48 5F49 5HMN
• Descriptor: aminoglycoside acetyltransferase meta-AAC0020, (1S,2S,3R,4S,6R)-4,6-diamino-3-{[(2S,3R)-3-amino-6-(aminomethyl)-3,4-dihydro-2H-pyran-2-yl]oxy}-2-hydroxycyclohexyl 3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranoside, MAGNESIUM ION, ... (6 entities in total)
• Functional Keywords: gnat fold, gcn5-n-acetyltransferase fold, acetyltransferase, aminoglycoside, sisomicin, antibiotic resistance, metagenome, soil, coenzyme a, structural genomics, center for structural genomics of infectious diseases, csgid, transferase-antibiotic complex, transferase/antibiotic
• Biological source: uncultured bacterium
• Total number of polymer chains: 4
• Total formula weight: 76039.15

Authors

Xu, Z.,Skarina, T.,Wawrzak, Z.,Stogios, P.J.,Yim, V.,Savchenko, A.,Anderson, W.F.,Center for Structural Genomics of Infectious Diseases (CSGID) (deposition date: 2016-11-23, release date: 2017-02-08, Last modification date: 2023-10-04)

• Primary citation: Xu, Z.,Stogios, P.J.,Quaile, A.T.,Forsberg, K.J.,Patel, S.,Skarina, T.,Houliston, S.,Arrowsmith, C.,Dantas, G.,Savchenko, A.; Structural and Functional Survey of Environmental Aminoglycoside Acetyltransferases Reveals Functionality of Resistance Enzymes.; ACS Infect Dis, 3:653-665, 2017; PubMed: 28756664; DOI: 10.1021/acsinfecdis.7b00068

Experimental method

X-RAY DIFFRACTION (1.52 Å)