5TRX

Room temperature structure of an extradiol ring-cleaving dioxygenase from B.fuscum determined using serial femtosecond crystallography

Summary for 5TRX

• Entry DOI: 10.2210/pdb5trx/pdb
• Descriptor: Homoprotocatechuate 2,3-dioxygenase, FE (II) ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: dioxygenase, 2-his-1-carboxylate facial triad, oxygen activation, double-flow focusing injector, oxidoreductase
• Biological source: Brevibacterium fuscum
• Total number of polymer chains: 4
• Total formula weight: 167426.55

Authors

Kovaleva, E.G.,Oberthuer, D.,Tolstikova, A.,Mariani, V. (deposition date: 2016-10-27, release date: 2017-03-22, Last modification date: 2023-10-04)

• Primary citation: Oberthuer, D.,Knoska, J.,Wiedorn, M.O.,Beyerlein, K.R.,Bushnell, D.A.,Kovaleva, E.G.,Heymann, M.,Gumprecht, L.,Kirian, R.A.,Barty, A.,Mariani, V.,Tolstikova, A.,Adriano, L.,Awel, S.,Barthelmess, M.,Dorner, K.,Xavier, P.L.,Yefanov, O.,James, D.R.,Nelson, G.,Wang, D.,Calvey, G.,Chen, Y.,Schmidt, A.,Szczepek, M.,Frielingsdorf, S.,Lenz, O.,Snell, E.,Robinson, P.J.,Sarler, B.,Belsak, G.,Macek, M.,Wilde, F.,Aquila, A.,Boutet, S.,Liang, M.,Hunter, M.S.,Scheerer, P.,Lipscomb, J.D.,Weierstall, U.,Kornberg, R.D.,Spence, J.C.H.,Pollack, L.,Chapman, H.N.,Bajt, S.; Double-flow focused liquid injector for efficient serial femtosecond crystallography; Sci. Rep., 7:44628-, 2017; DOI: 10.1038/srep44628

Experimental method

X-RAY DIFFRACTION (2.38 Å)