5MG1

Structure of the photosensory module of Deinococcus phytochrome by serial femtosecond X-ray crystallography

Summary for 5MG1

• Entry DOI: 10.2210/pdb5mg1/pdb
• Related: 4Q0J
• Descriptor: Bacteriophytochrome, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid (3 entities in total)
• Functional Keywords: photosensory module, phytochrome, biliverdin, photoreceptor, transferase
• Biological source: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
• Total number of polymer chains: 1
• Total formula weight: 57279.86

Authors

Burgie, E.S.,Fuller, F.D.,Gul, S.,Young, I.D.,Brewster, A.S.,Clinger, J.,Andi, B.,Stan, C.,Allaire, M.,Nelsen, S.,Alonso-Mori, R.,Phillips Jr., G.N.,Sauter, N.K.,Kern, J.,Yachandra, V.K.,Yano, J.,Vierstra, R.D.,Orville, A.M. (deposition date: 2016-11-20, release date: 2017-02-22, Last modification date: 2024-01-17)

• Primary citation: Fuller, F.D.,Gul, S.,Chatterjee, R.,Burgie, E.S.,Young, I.D.,Lebrette, H.,Srinivas, V.,Brewster, A.S.,Michels-Clark, T.,Clinger, J.A.,Andi, B.,Ibrahim, M.,Pastor, E.,de Lichtenberg, C.,Hussein, R.,Pollock, C.J.,Zhang, M.,Stan, C.A.,Kroll, T.,Fransson, T.,Weninger, C.,Kubin, M.,Aller, P.,Lassalle, L.,Brauer, P.,Miller, M.D.,Amin, M.,Koroidov, S.,Roessler, C.G.,Allaire, M.,Sierra, R.G.,Docker, P.T.,Glownia, J.M.,Nelson, S.,Koglin, J.E.,Zhu, D.,Chollet, M.,Song, S.,Lemke, H.,Liang, M.,Sokaras, D.,Alonso-Mori, R.,Zouni, A.,Messinger, J.,Bergmann, U.,Boal, A.K.,Bollinger, J.M.,Krebs, C.,Hogbom, M.,Phillips, G.N.,Vierstra, R.D.,Sauter, N.K.,Orville, A.M.,Kern, J.,Yachandra, V.K.,Yano, J.; Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers.; Nat. Methods, 14:443-449, 2017; PubMed: 28250468; DOI: 10.1038/nmeth.4195

Experimental method

X-RAY DIFFRACTION (3.3 Å)