4ZT0

Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution

Summary for 4ZT0

Entry DOI	10.2210/pdb4zt0/pdb
Related	4ZT9
Descriptor	CRISPR-associated endonuclease Cas9, single-guide RNA (2 entities in total)
Functional Keywords	crispr-cas9, bacteria adaptive immunity, dna endonuclease, hydrolase-rna complex, genome editing and regulation, hydrolase/rna
Biological source	Streptococcus pyogenes More
Total number of polymer chains	4
Total formula weight	374612.00
Authors	Jiang, F.,Doudna, J.A. (deposition date: 2015-05-14, release date: 2015-07-08)
Primary citation	Jiang, F.,Zhou, K.,Ma, L.,Gressel, S.,Doudna, J.A. A Cas9-guide RNA complex preorganized for target DNA recognition. Science, 348:1477-1481, 2015 Cited by PubMed: 26113724 DOI: 10.1126/science.aab1452 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.9 Å)

Structure validation