4Q3C

PylD cocrystallized with L-Lysine-Ne-L-lysine and NAD+

4Q3C の概要

• エントリーDOI: 10.2210/pdb4q3c/pdb
• 関連するPDBエントリー: 3T7V 4FFP 4Q39 4Q3A 4Q3B 4Q3D 4Q3E
• 分子名称: PylD, pyrrolysine synthase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, SODIUM ION, ... (9 entities in total)
• 機能のキーワード: rossmann fold, dehydrogenase, pyrrolysine, oxidoreductase
• 由来する生物種: Methanosarcina barkeri
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 116456.31

構造登録者

Quitterer, F.,Beck, P.,Bacher, A.,Groll, M. (登録日: 2014-04-11, 公開日: 2014-04-23, 最終更新日: 2024-10-30)

主引用文献

Quitterer, F.,Beck, P.,Bacher, A.,Groll, M.
The Formation of Pyrroline and Tetrahydropyridine Rings in Amino Acids Catalyzed by Pyrrolysine Synthase (PylD).
Angew.Chem.Int.Ed.Engl., 53:8150-8153, 2014

PubMed Abstract: The dehydrogenase PylD catalyzes the ultimate step of the pyrrolysine pathway by converting the isopeptide L-lysine-Nε-3R-methyl-D-ornithine to the 22nd proteinogenic amino acid. In this study, we demonstrate how PylD can be harnessed to oxidize various isopeptides to novel amino acids by combining chemical synthesis with enzyme kinetics and X-ray crystallography. The data enable a detailed description of the PylD reaction trajectory for the biosynthesis of pyrroline and tetrahydropyridine rings as constituents of pyrrolysine analogues.

PubMed: 24916332
DOI: 10.1002/anie.201402595

実験手法

X-RAY DIFFRACTION (2.1 Å)